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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets

  • Authors:
    • Wei Zhang
    • Hui Chen
    • Dian-Lei Liu
    • Hong Li
    • Jiang Luo
    • Jian-Hong Zhang
    • Ye Li
    • Kang-Jie Chen
    • Hong-Fei Tong
    • Sheng-Zhang Lin
  • View Affiliations / Copyright

    Affiliations: The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310000, P.R. China, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, P.R. China, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, P.R. China, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310000, P.R. China
  • Pages: 1189-1196
    |
    Published online on: February 22, 2013
       https://doi.org/10.3892/ijo.2013.1839
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Abstract

The aim of this study was to evaluate whether emodin can overcome the chemoresistance of the gemcitabine-resistant cancer cell line (Bxpc-3/Gem) in vitro. The cell line Bxpc-3/Gem was derived from the human pancreatic cancer cell line Bxpc-3. We found that Bxpc-3/Gem cells were characterized by a series of morphological changes with a resistance index of 43.51 comparing with the parental cell line. Emodin reduced Bxpc-3/Gem cell proliferation in a dose-dependent manner. Emodin and gemcitabine combination treatments resulted in decreased cell proliferation and increased apoptosis in Bxpc-3/Gem cells. In addition, combination treatments resulted in downregulation of gene and protein expression of MDR-1 (P-gp), NF-κB, XIAP, survivin, as well as inhibition of NF-κB activity and P-gp function. These observations suggest that emodin may sensitize the pancreatic cancer gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine therapy via inhibition of survival signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Chen H, Liu D, Li H, Luo J, Zhang J, Li Y, Chen K, Tong H, Lin S, Lin S, et al: Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets. Int J Oncol 42: 1189-1196, 2013.
APA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J. ... Lin, S. (2013). Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets. International Journal of Oncology, 42, 1189-1196. https://doi.org/10.3892/ijo.2013.1839
MLA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42.4 (2013): 1189-1196.
Chicago
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42, no. 4 (2013): 1189-1196. https://doi.org/10.3892/ijo.2013.1839
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Chen H, Liu D, Li H, Luo J, Zhang J, Li Y, Chen K, Tong H, Lin S, Lin S, et al: Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets. Int J Oncol 42: 1189-1196, 2013.
APA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J. ... Lin, S. (2013). Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets. International Journal of Oncology, 42, 1189-1196. https://doi.org/10.3892/ijo.2013.1839
MLA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42.4 (2013): 1189-1196.
Chicago
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42, no. 4 (2013): 1189-1196. https://doi.org/10.3892/ijo.2013.1839
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