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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Immune escape of AKT overexpressing ovarian cancer cells

  • Authors:
    • Jens C. Hahne
    • Susanne R. Meyer
    • Stepan Gambaryan
    • Ulrich Walter
    • Johannes Dietl
    • Jörg B. Engel
    • Arnd Honig
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, University Hospital of Würzburg, D-97080 Würzburg, Germany, Institute for Clinical Biochemistry and Pathobiochemistry, University of Wuerzburg, D-97080 Würzburg, Germany, Centre for Thrombosis and Haemostasis, University of Mainz, D-55131 Mainz, Germany, Department of Gynecology, University of Regensburg, D-93055 Regensburg, Germany
  • Pages: 1630-1635
    |
    Published online on: March 4, 2013
       https://doi.org/10.3892/ijo.2013.1846
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Abstract

Platinum-resistance is the most crucial problem for treatment of ovarian cancer. There is a clinical need for new treatment strategies which overcome platinum resistance. As survival is strongly influenced by immunological parameters, immunotherapeutic strategies appear promising. Therefore a better understanding of the interaction between ovarian tumour cells and cells of the immune system is a necessary prerequisite. In the present study we aimed to enlighten the interactions between platinum resistant and platinum sensitive ovarian cancer cells and natural-killer (NK)-cells. Modified FATAL assay was used for determining the killing efficiency of NK-cells for the parental A2780 cells and the cis-platinum resistant A2780cis human ovarian cancer cells. Expression of pro- and anti-apoptotic genes as well as ligands involved in NK-cell receptor recognition were analysed by RT-PCR and flow cytometric analysis. The efficiency of NK mediated cell lysis differs between A2780 cells and the cis-platinum-resistant A2780cis cells. A2780cis cells are less accessible for NK-cell mediated killing. Based on this observation we characterized the molecular basis for resistance mechanisms. Besides an increase in anti-apoptotic genes (especially CIAP-1 and -2) that probably render A2780cis cells more resistant against apoptosis an increased amount of soluble MICA/B seems to be responsible for the lower killing rate of platinum-resistant A2780cis cells compared to their parental A2780 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Hahne JC, Meyer SR, Gambaryan S, Walter U, Dietl J, Engel JB and Honig A: Immune escape of AKT overexpressing ovarian cancer cells. Int J Oncol 42: 1630-1635, 2013.
APA
Hahne, J.C., Meyer, S.R., Gambaryan, S., Walter, U., Dietl, J., Engel, J.B., & Honig, A. (2013). Immune escape of AKT overexpressing ovarian cancer cells. International Journal of Oncology, 42, 1630-1635. https://doi.org/10.3892/ijo.2013.1846
MLA
Hahne, J. C., Meyer, S. R., Gambaryan, S., Walter, U., Dietl, J., Engel, J. B., Honig, A."Immune escape of AKT overexpressing ovarian cancer cells". International Journal of Oncology 42.5 (2013): 1630-1635.
Chicago
Hahne, J. C., Meyer, S. R., Gambaryan, S., Walter, U., Dietl, J., Engel, J. B., Honig, A."Immune escape of AKT overexpressing ovarian cancer cells". International Journal of Oncology 42, no. 5 (2013): 1630-1635. https://doi.org/10.3892/ijo.2013.1846
Copy and paste a formatted citation
x
Spandidos Publications style
Hahne JC, Meyer SR, Gambaryan S, Walter U, Dietl J, Engel JB and Honig A: Immune escape of AKT overexpressing ovarian cancer cells. Int J Oncol 42: 1630-1635, 2013.
APA
Hahne, J.C., Meyer, S.R., Gambaryan, S., Walter, U., Dietl, J., Engel, J.B., & Honig, A. (2013). Immune escape of AKT overexpressing ovarian cancer cells. International Journal of Oncology, 42, 1630-1635. https://doi.org/10.3892/ijo.2013.1846
MLA
Hahne, J. C., Meyer, S. R., Gambaryan, S., Walter, U., Dietl, J., Engel, J. B., Honig, A."Immune escape of AKT overexpressing ovarian cancer cells". International Journal of Oncology 42.5 (2013): 1630-1635.
Chicago
Hahne, J. C., Meyer, S. R., Gambaryan, S., Walter, U., Dietl, J., Engel, J. B., Honig, A."Immune escape of AKT overexpressing ovarian cancer cells". International Journal of Oncology 42, no. 5 (2013): 1630-1635. https://doi.org/10.3892/ijo.2013.1846
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