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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells

  • Authors:
    • Bo Huang
    • Wenting Luo
    • Limei Sun
    • Qingfu Zhang
    • Lili Jiang
    • Jihong Chang
    • Xueshan Qiu
    • Enhua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China
  • Pages: 1734-1742
    |
    Published online on: March 21, 2013
       https://doi.org/10.3892/ijo.2013.1861
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Abstract

MicroRNAs (miRNAs) function as genetic modulators that regulate gene expression, and are, thus, involved in a wide range of biological roles, including tumor cell migration and invasion. MiR-125a-3p is a mature form of miR-125a, derived from the 3'-end of pre-miR-125a. Our group has previously reported that miR-125a-3p functions as a tumor suppressor gene that inhibits the migration and invasion of lung cancer cells. Here, we report the discovery of a new regulatory layer of the RhoA-actomyosin pathway through which miR-125a-3p controls tumor cell migration. Overexpression of miR-125a-3p by transfection of sense‑miR‑125a-3p resulted in decreased RhoA protein levels, while the levels of RhoA mRNA remained constant. The concentrations of both RhoA-GTP protein and actin filaments decreased after miR-125a-3p overexpression in the A549 lung cancer cell line. Conversely, knockdown of miR-125a-3p by transfection of antisense-miR-125a-3p resulted in increased RhoA protein levels while the levels of RhoA mRNA remained unchanged. However, the concentration of both RhoA-GTP protein and actin filaments increased. To further demonstrate that RhoA is a potential target of miR‑125a-3p, luciferase reporter constructs containing the RhoA 3'UTR demonstrated reduced reporter activity after ectopic expression of miR-125a-3p. Moreover, luciferase reporter constructs containing the RhoA 3'UTR mutant did not show significantly changed reporter activity. Furthermore, A549 cells demonstrated reduced migratory capacity after treatment with the Rho inhibitor CT04. Our results indicate that the loss of miR-125a‑3p-controlled regulation of the RhoA-actomyosin pathway can lead to increased migration of tumor cells because of the upregulation of RhoA expression. In particular, an increased intracellular concentration of RhoA-GTP protein in A549 cells leads to the accumulation of actin filaments. These results provide new insights into the role of the miR-125a family in lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Huang B, Luo W, Sun L, Zhang Q, Jiang L, Chang J, Qiu X and Wang E: MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells. Int J Oncol 42: 1734-1742, 2013.
APA
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J. ... Wang, E. (2013). MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells. International Journal of Oncology, 42, 1734-1742. https://doi.org/10.3892/ijo.2013.1861
MLA
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J., Qiu, X., Wang, E."MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells". International Journal of Oncology 42.5 (2013): 1734-1742.
Chicago
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J., Qiu, X., Wang, E."MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells". International Journal of Oncology 42, no. 5 (2013): 1734-1742. https://doi.org/10.3892/ijo.2013.1861
Copy and paste a formatted citation
x
Spandidos Publications style
Huang B, Luo W, Sun L, Zhang Q, Jiang L, Chang J, Qiu X and Wang E: MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells. Int J Oncol 42: 1734-1742, 2013.
APA
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J. ... Wang, E. (2013). MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells. International Journal of Oncology, 42, 1734-1742. https://doi.org/10.3892/ijo.2013.1861
MLA
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J., Qiu, X., Wang, E."MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells". International Journal of Oncology 42.5 (2013): 1734-1742.
Chicago
Huang, B., Luo, W., Sun, L., Zhang, Q., Jiang, L., Chang, J., Qiu, X., Wang, E."MiRNA-125a-3p is a negative regulator of the RhoA-actomyosin pathway in A549 cells". International Journal of Oncology 42, no. 5 (2013): 1734-1742. https://doi.org/10.3892/ijo.2013.1861
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