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Article

Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA

  • Authors:
    • Akiko Kawai
    • Toshiyuki Kobayashi
    • Okio Hino
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Oncology, Juntendo University, School of Medicine, Tokyo 113-8421, Japan
  • Pages: 1597-1604
    |
    Published online on: March 21, 2013
       https://doi.org/10.3892/ijo.2013.1862
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Abstract

Birt-Hogg-Dubé syndrome (BHDS) is an autosomal dominantly inherited disease characterized by spontaneous pneumothorax, hair folliculomas and renal tumors. The responsible gene, BHD, is a tumor suppressor and encodes folliculin. Folliculin is an evolutionarily conserved protein (~67 kDa) with no apparent functional motif and its role has not yet been fully elucidated. In this study, we found that knockdown of BHD increased the levels of cyclin D1 in HeLa cells. A reporter assay with the cyclin D1 gene (CCND1) promoter region indicated that this increase was not caused by activation of transcription through known cis-acting elements. We examined the possibility of post-transcriptional mechanism using reporter constructs containing fragments of the cyclin D1 3' untranslated region (3'UTR). Transfection of control cells with a construct carrying a medial 1.3 kb 3'UTR fragment resulted in a significant reduction in luciferase activity. This effect was largely prevented by knockdown of BHD. Our results suggest that the post-transcriptional regulation of the CCND1 expression by BHD may be associated with microRNA(s) or RNA binding protein(s) that bind to the 3'UTR.
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Copy and paste a formatted citation
Spandidos Publications style
Kawai A, Kobayashi T and Hino O: Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. Int J Oncol 42: 1597-1604, 2013.
APA
Kawai, A., Kobayashi, T., & Hino, O. (2013). Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. International Journal of Oncology, 42, 1597-1604. https://doi.org/10.3892/ijo.2013.1862
MLA
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42.5 (2013): 1597-1604.
Chicago
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42, no. 5 (2013): 1597-1604. https://doi.org/10.3892/ijo.2013.1862
Copy and paste a formatted citation
x
Spandidos Publications style
Kawai A, Kobayashi T and Hino O: Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. Int J Oncol 42: 1597-1604, 2013.
APA
Kawai, A., Kobayashi, T., & Hino, O. (2013). Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. International Journal of Oncology, 42, 1597-1604. https://doi.org/10.3892/ijo.2013.1862
MLA
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42.5 (2013): 1597-1604.
Chicago
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42, no. 5 (2013): 1597-1604. https://doi.org/10.3892/ijo.2013.1862
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