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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector

  • Authors:
    • Danqi Chen
    • Ruixiu Zhang
    • Wei Shen
    • Hao Fu
    • Shanshan Liu
    • Kailai Sun
    • Xiuju Sun
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics, China Medical University, Shenyang 110001, P.R. China
  • Pages: 1763-1769
    |
    Published online on: March 28, 2013
       https://doi.org/10.3892/ijo.2013.1872
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Abstract

Our previous study using suppression subtractive hybridization (SSH), cDNA microarray and semi-quantitative RT-PCR showed that RPS12 was overexpressed in gastric cancer and it was closely related to metastasis. However, the role of RPS12 in gastric cancer is not clear, which led us to conduct the current study to further investigate the effects of RPS12 on the proliferation and migration of gastric cancer cells, and also to explore the underlying molecular mechanisms. RNA interference was used to inhibit the expression of RPS12. The expression of RPS12 and S100A4 in gastric cancer cells was determined using semi-quantitative RT-PCR and western blot analysis. Cell proliferation and migration were detected by MTT and transwell assay, respectively. In addition, the promoter activity of S100A4 was measured by a Dual-Luciferase Reporter Assay System. We found that RNAi‑mediated RPS12 downregulation led to reduced proliferation and migration of BGC823 and SGC7901 gastric cancer cells. Further results showed that RPS12 inhibition led to reduced S100A4 expression and decreased promoter activity of S100A4 in BGC823 cells. We demonstrated that ectopic expression of S100A4 reversed the reduced proliferation and migration ability after RPS12 inhibition in BGC823 cells. Our findings provide the first demonstration that RPS12 plays important roles in regulating the proliferation and migration of gastric cancer cells. S100A4 can mediate the effects of RPS12 as a downstream effector.
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1. 

Sun XJ, Sun KL, Zheng ZH, Hao DM, Fu WN, Xu HM, Chen JQ and Li XM: Analysis of gene expression profiles for distinct stages of intestinal-type gastric cancer using suppression subtractive hybridization and cDNA microarray. Scand J Gastroenterol. 40:1244–1245. 2005. View Article : Google Scholar : PubMed/NCBI

2. 

Sun XJ, Hao DM, Zheng ZH, Fu H, Xu HM, Wang MX and Sun KL: Screening and analysis of associated genes in the carcinogenesis and progression of gastric cancer. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 22:31–34. 2005.(In Chinese).

3. 

Yan Y, Fu H, Hua J, Zhang RX, Chen DQ, Sun KL and Sun XJ: The effects of silencing the RPS12 gene by RNAi on apoptosis of gastric cancer cells and the possible molecular mechanism. Chin J Clin Oncol. 35:1415–1418. 2008.(In Chinese).

4. 

Hua J, Chen DQ, Fu H, Zhang RX, Shen W, Liu SS, Sun KL and Sun XJ: Short haripin RNA-mediated inhibition of S100A4 promotes apoptosis and suppresses proliferation of BGC823 gastric cancer cells in vitro and in vivo. Cancer Lett. 292:41–47. 2010. View Article : Google Scholar

5. 

Zhang RX, Fu H, Chen DQ, Hua J, Hu YP, Sun KL and Sun XJ: Subcellular distribution of S100A4 and its transcriptional regulation under hypoxic conditions in gastric cancer cell line BGC823. Cancer Sci. 101:1141–1146. 2010. View Article : Google Scholar : PubMed/NCBI

6. 

Shen W, Chen DQ, Fu H, Liu SS, Sun KL and Sun XJ: S100A4 protects gastric cancer cells from anoikis through regulation of αv and α5 integrin. Cancer Sci. 102:1014–1018. 2011.PubMed/NCBI

7. 

Frank J: The ribosome - a macromolecular machine par excellence. Chem Biol. 7:R133–R141. 2000. View Article : Google Scholar : PubMed/NCBI

8. 

Lai MD and Xu J: Ribosomal proteins and colorectal cancer. Curr Genomics. 8:43–49. 2007. View Article : Google Scholar : PubMed/NCBI

9. 

Kobayashi T, Sasaki Y, Oshima Y, Yamamoto H, Mita H, Suzuki H, Toyota M, Tokino T, Itoh F, Imai K and Shinomura Y: Activation of the ribosomal protein L13 gene in human gastrointestinal cancer. Int J Mol Med. 18:161–170. 2006.PubMed/NCBI

10. 

Huang CJ, Chen CC, Yang SH, Chang CC, Sun HL, Cheng YC, Liu CC, Lin SC and Lin CM: Faecal ribosomal protein L19 is a genetic prognostic factor for survival in colorectal cancer. J Cell Mol Med. 12:1936–1943. 2008. View Article : Google Scholar : PubMed/NCBI

11. 

Cheng Q, Lau WM, Chew SH, Ho TH, Tay SK and Hui KM: Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix. Br J Cancer. 86:274–281. 2002. View Article : Google Scholar : PubMed/NCBI

12. 

Wang M, Hu Y, Amatangelo MD and Stearns ME: Role of ribosomal protein RPS2 in controlling let-7a expression in human prostate cancer. Mol Cancer Res. 9:36–50. 2011. View Article : Google Scholar : PubMed/NCBI

13. 

Kim JH, You KR, Kim IH, Cho BH, Kim CY and Kim DG: Over-expression of the ribosomal protein L36a gene is associated with cellular proliferation in hepatocellular carcinoma. Hepatology. 39:129–138. 2004. View Article : Google Scholar : PubMed/NCBI

14. 

Sridharan S and Basu A: S6 kinase 2 promotes breast cancer cell survival via AKT. Cancer Res. 71:2590–2599. 2011. View Article : Google Scholar : PubMed/NCBI

15. 

Kasai H, Nadano D, Hidaka E, Higuchi K, Kawakubo M, Sato TA and Nakayama J: Differential expression of ribosomal proteins in human normal and neoplastic colorectum. J Histochem Cytochem. 51:567–574. 2003. View Article : Google Scholar : PubMed/NCBI

16. 

Kondoh N, Shuda M, Tanaka K, Wakatsuki T, Hada A and Yamamoto M: Enhanced expression of S8, L12, L23a, L27 and L30 ribosomal protein mRNAs in human hepatocellular carcinoma. Anticancer Res. 21:2429–2433. 2003.PubMed/NCBI

17. 

Shriver SP, Shriver MD, Tirpak DL, Bloch LM, Hunt JD, Ferrell RE and Siegfried JM: Trinucleotide repeat length variation in the human ribosomal protein L14 gene (RPL14): localization to 3p21.3 and loss of heterozygosity in lung and oral cancers. Mutat Res. 406:9–23. 1998.PubMed/NCBI

18. 

Pogue-Geile K, Geiser JR, Shu M, Miller C, Wool IG, Meisler AI and Pipas JM: Ribosomal protein genes are over-expressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Mol Cell Biol. 11:3842–3849. 1998.PubMed/NCBI

19. 

O’Donohue MF, Choesmel V, Faubladier M, Fichant G and Gleizes PE: Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits. J Cell Biology. 190:853–866. 2010.PubMed/NCBI

20. 

Wang H, Zhao LN, Li KZ, Ling R, Li XJ and Wang L: Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer. BMC Cancer. 6:912006. View Article : Google Scholar : PubMed/NCBI

21. 

Khanna N, Sen S, Sharma H and Singh N: S29 ribosomal protein induces apoptosis in H520 cells and sensitizes them to chemotherapy. Biochem Biophys Res Commun. 304:26–35. 2003. View Article : Google Scholar : PubMed/NCBI

22. 

Wu Q, Gou Y, Wang Q, Jin H, Cui L, Zhang Y, He L, Wang J, Nie Y, Shi Y and Fan D: Downregulation of RPL6 by siRNA inhibits proliferation and cell cycle progression of human gastric cancer cell lines. PLoS One. 6:e264012011. View Article : Google Scholar : PubMed/NCBI

23. 

Forst B, Hansen MT, Klingelhöfer J, Møller HD, Nielsen GH, Grum-Schwensen B, Ambartsumian N, Lukanidin E and Grigorian M: Metastasis-inducing S100A4 and RANTES cooperate in promoting tumor progression in mice. PLoS One. 5:e103742010. View Article : Google Scholar : PubMed/NCBI

24. 

Kwon YW, Chang IH, Kim KD, Kim YS, Myung SC, Kim MK and Kim TH: Significance of S100A2 and S100A4 expression in the progression of prostate adenocarcinoma. Korean J Urol. 51:456–462. 2010. View Article : Google Scholar : PubMed/NCBI

25. 

Wang YY, Ye ZY, Zhao ZS, Tao HQ and Chu YQ: High-level expression of S100A4 correlates with lymph node metastasis and poor prognosis in patients with gastric cancer. Ann Surg Oncol. 17:89–97. 2010. View Article : Google Scholar : PubMed/NCBI

26. 

Dai MS, Sears R and Lu H: Feedback regulation of c-Myc by ribosomal protein L11. Cell Cycle. 6:2735–2741. 2007. View Article : Google Scholar : PubMed/NCBI

27. 

Chen D, Zhang Z, Li M, Wang W, Li Y, Rayburn ER, Hill DL, Wang H and Zhang R: Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene. 26:5029–5037. 2007. View Article : Google Scholar : PubMed/NCBI

28. 

Dai MS and Lu H: Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5. J Biol Chem. 279:44475–44482. 2004. View Article : Google Scholar : PubMed/NCBI

29. 

Dai MS, Zeng SX, Jin Y, Sun XX, David L and Lu H: Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition. Mol Cell Biol. 24:7654–7668. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen D, Zhang R, Shen W, Fu H, Liu S, Sun K and Sun X: RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector. Int J Oncol 42: 1763-1769, 2013.
APA
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., & Sun, X. (2013). RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector. International Journal of Oncology, 42, 1763-1769. https://doi.org/10.3892/ijo.2013.1872
MLA
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., Sun, X."RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector". International Journal of Oncology 42.5 (2013): 1763-1769.
Chicago
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., Sun, X."RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector". International Journal of Oncology 42, no. 5 (2013): 1763-1769. https://doi.org/10.3892/ijo.2013.1872
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Zhang R, Shen W, Fu H, Liu S, Sun K and Sun X: RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector. Int J Oncol 42: 1763-1769, 2013.
APA
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., & Sun, X. (2013). RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector. International Journal of Oncology, 42, 1763-1769. https://doi.org/10.3892/ijo.2013.1872
MLA
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., Sun, X."RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector". International Journal of Oncology 42.5 (2013): 1763-1769.
Chicago
Chen, D., Zhang, R., Shen, W., Fu, H., Liu, S., Sun, K., Sun, X."RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector". International Journal of Oncology 42, no. 5 (2013): 1763-1769. https://doi.org/10.3892/ijo.2013.1872
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