MicroRNA-21 regulates the migration and invasion of a stem-like population in hepatocellular carcinoma

Zhou,Liang; Yang,Zhao-Xu; Song,Wen-Jie; Li,Qing-Jun; Yang,Fan; Wang,De-Sheng; Zhang,Ning; Dou,Ke-Feng

doi:10.3892/ijo.2013.1965

August 2013 Volume 43 Issue 2

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August 2013 Volume 43 Issue 2

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Article

MicroRNA-21 regulates the migration and invasion of a stem-like population in hepatocellular carcinoma

Authors:
- Liang Zhou
- Zhao-Xu Yang
- Wen-Jie Song
- Qing-Jun Li
- Fan Yang
- De-Sheng Wang
- Ning Zhang
- Ke-Feng Dou
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Affiliations: Department of General Surgery, The 155 Central Hospital of PLA, Kaifeng, He'nan 471000, P.R. China, Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shannxi 710032, P.R. China
Pages: 661-669
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Published online on: May 27, 2013

https://doi.org/10.3892/ijo.2013.1965
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Abstract

Due to invasion and intrahepatic metastasis, the prognosis for patients with hepatocellular carcinoma (HCC) is poor. However, the mechanisms underlying these processes of HCC remain unclear. Cancer stem cells may be involved in early systemic dissemination and metastasis formation and side population (SP) cells isolated from diverse cancer cells possess stem cell-like properties. However, the mechanisms involved in migration and invasion of cancer stem cells are not well understood. In this study, we identified and isolated populations of SP cells from HCC cell lines using flow cytometry. SP cells showed higher levels of migration and invasion capability. Higher expression of miR-21 was observed in SP cells. Silencing of miR-21 led to a reduction in the migration and invasion of these cells and overexpression of miR-21 can increase in cell migration and invasion. Overexpression of miR-21 did not cause degradation of PTEN or RECK or PDCD4 mRNA but drastically inhibited its protein expression. Consistent with these results, silencing miR-21 increased the levels of PTEN, RECK and PDCD4 protein, respectively. The role of silencing miR-21 was partially attenuated by silencing of PTEN or RECK or PDCD4 mRNA. The results of this study revealed the aberrant expression of miR-21 in SP cells and showed that miR-21 regulates the expression of multiple target proteins that are associated with tumor dissemination. MiR-21 is a pro-metastatic miRNA in SP cells and raises the possibility that therapy of HCC may be improved by pharmaceutical strategies directed towards miR-21.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA-21 regulates the migration and invasion of a stem-like population in hepatocellular carcinoma

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