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Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth

  • Authors:
    • Carol O'Callaghan
    • Liam J. Fanning
    • Aileen Houston
    • Orla P. Barry
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Therapeutics, University College Cork, Ireland, Department of Medicine, University College Cork, Ireland
    Copyright: © O'Callaghan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 405-415
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    Published online on: May 29, 2013
       https://doi.org/10.3892/ijo.2013.1968
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Abstract

Oesophageal cancer is an aggressive tumour which responds poorly to both chemotherapy and radiation therapy and has a poor prognosis. Thus, a greater understanding of the biology of oesophageal cancer is needed in order to identify novel therapeutic targets. Among these targets p38 MAPK isoforms are becoming increasingly important for a variety of cellular functions. The physiological functions of p38α and -β are now well documented in contrast to -γ and -δ which are comparatively under-studied and ill-defined. A major obstacle to deciphering the role(s) of the latter two p38 isoforms is the lack of specific chemical activators and inhibitors. In this study, we analysed p38 MAPK isoform expression in oesophageal cancer cell lines as well as human normal and tumour tissue. We observed specifically differential p38δ expression. The role(s) of p38δ and active (phosphorylated) p38δ (p-p38δ) in oesophageal squamous cell carcinoma (OESCC) was delineated using wild-type p38δ as well as active p-p38δ, generated by fusing p38δ to its upstream activator MKK6b(E) via a decapeptide (Gly-Glu)5 linker. OESCC cell lines which are p38δ-negative (KE-3 and -8) grew more quickly than cell lines (KE-6 and -10) which express endogenous p38δ. Re-introduction of p38δ resulted in a time-dependent decrease in OESCC cell proliferation which was exacerbated with p-p38δ. In addition, we observed that p38δ and p-p38δ negatively regulated OESCC cell migration in vitro. Finally both p38δ and p-p38δ altered OESCC anchorage-independent growth. Our results suggest that p38δ and p-p38δ have a role in the suppression of OESCC. Our research may provide a new potential target for the treatment of oesophageal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
O'Callaghan C, Fanning LJ, Houston A and Barry OP: Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth. Int J Oncol 43: 405-415, 2013.
APA
O'Callaghan, C., Fanning, L.J., Houston, A., & Barry, O.P. (2013). Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth. International Journal of Oncology, 43, 405-415. https://doi.org/10.3892/ijo.2013.1968
MLA
O'Callaghan, C., Fanning, L. J., Houston, A., Barry, O. P."Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth". International Journal of Oncology 43.2 (2013): 405-415.
Chicago
O'Callaghan, C., Fanning, L. J., Houston, A., Barry, O. P."Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth". International Journal of Oncology 43, no. 2 (2013): 405-415. https://doi.org/10.3892/ijo.2013.1968
Copy and paste a formatted citation
x
Spandidos Publications style
O'Callaghan C, Fanning LJ, Houston A and Barry OP: Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth. Int J Oncol 43: 405-415, 2013.
APA
O'Callaghan, C., Fanning, L.J., Houston, A., & Barry, O.P. (2013). Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth. International Journal of Oncology, 43, 405-415. https://doi.org/10.3892/ijo.2013.1968
MLA
O'Callaghan, C., Fanning, L. J., Houston, A., Barry, O. P."Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth". International Journal of Oncology 43.2 (2013): 405-415.
Chicago
O'Callaghan, C., Fanning, L. J., Houston, A., Barry, O. P."Loss of p38δ mitogen-activated protein kinase expression promotes oesophageal squamous cell carcinoma proliferation, migration and anchorage-independent growth". International Journal of Oncology 43, no. 2 (2013): 405-415. https://doi.org/10.3892/ijo.2013.1968
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