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Article

Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells

  • Authors:
    • Michelly C. Pereira
    • Simone A. de Bessa-Garcia
    • Ravshan Burikhanov
    • Ana Carolina Pavanelli
    • Lourival Antunes
    • Vivek M. Rangnekar
    • Maria A. Nagai
  • View Affiliations / Copyright

    Affiliations: Discipline of Oncology, Department of Radiology and Oncology, Faculty of Medicine, University of São Paulo, CEP 01246-903, São Paulo, Brazil, Markey Cancer Center, University of Kentucky, Lexington, KY, USA
  • Pages: 531-538
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    Published online on: June 12, 2013
       https://doi.org/10.3892/ijo.2013.1983
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Abstract

Experimental evidence indicates that prostate apoptosis response-4 (Par-4, also known as PAWR) is a key regulator of cancer cell survival and may be a target for cancer-selective targeted therapeutics. Par-4 was first identified in prostate cancer cells undergoing apoptosis. Both intracellular and extracellular Par-4 have been implicated in apoptosis. Relatively little is known about the role of Par-4 in breast cancer cell apoptosis. In this study, we sought to investigate the effects of Par-4 expression on cell proliferation, apoptosis and drug sensitivity in breast cancer cells. MCF-7 cells were stably transfected with expression vectors for Par-4, or transiently transfected with siRNA for Par-4 knockdown. Cell proliferation assays were performed using MTT and apoptosis was evaluated using acridine orange staining, fluorescence microscopy and flow cytometry. Par-4 overexpression reduced MCF-7 proliferation rates. Conversely, Par-4 knockdown led to increased MCF-7 proliferation. Par-4 downregulation also led to increased BCL-2 and reduced BID expression. Par-4 overexpression did not affect the cell cycle profile. However, MCF-7 cells with increased Par-4 expression showed reduced ERK phosphorylation, suggesting that the inhibition of cell proliferation promoted by Par-4 may be mediated by the MAPK/ERK1/2 pathway. MCF-7 cells with increased Par-4 expression showed a marginal increase in early apoptotic cells. Importantly, we found that Par-4 expression modulates apoptosis in response to docetaxel in MCF7 breast cancer cells. Par-4 exerts growth inhibitory effects on breast cancer cells and chemosensitizes them to docetaxel.
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Copy and paste a formatted citation
Spandidos Publications style
Pereira MC, de Bessa-Garcia SA, Burikhanov R, Pavanelli AC, Antunes L, Rangnekar VM and Nagai MA: Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells. Int J Oncol 43: 531-538, 2013.
APA
Pereira, M.C., de Bessa-Garcia, S.A., Burikhanov, R., Pavanelli, A.C., Antunes, L., Rangnekar, V.M., & Nagai, M.A. (2013). Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells. International Journal of Oncology, 43, 531-538. https://doi.org/10.3892/ijo.2013.1983
MLA
Pereira, M. C., de Bessa-Garcia, S. A., Burikhanov, R., Pavanelli, A. C., Antunes, L., Rangnekar, V. M., Nagai, M. A."Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells". International Journal of Oncology 43.2 (2013): 531-538.
Chicago
Pereira, M. C., de Bessa-Garcia, S. A., Burikhanov, R., Pavanelli, A. C., Antunes, L., Rangnekar, V. M., Nagai, M. A."Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells". International Journal of Oncology 43, no. 2 (2013): 531-538. https://doi.org/10.3892/ijo.2013.1983
Copy and paste a formatted citation
x
Spandidos Publications style
Pereira MC, de Bessa-Garcia SA, Burikhanov R, Pavanelli AC, Antunes L, Rangnekar VM and Nagai MA: Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells. Int J Oncol 43: 531-538, 2013.
APA
Pereira, M.C., de Bessa-Garcia, S.A., Burikhanov, R., Pavanelli, A.C., Antunes, L., Rangnekar, V.M., & Nagai, M.A. (2013). Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells. International Journal of Oncology, 43, 531-538. https://doi.org/10.3892/ijo.2013.1983
MLA
Pereira, M. C., de Bessa-Garcia, S. A., Burikhanov, R., Pavanelli, A. C., Antunes, L., Rangnekar, V. M., Nagai, M. A."Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells". International Journal of Oncology 43.2 (2013): 531-538.
Chicago
Pereira, M. C., de Bessa-Garcia, S. A., Burikhanov, R., Pavanelli, A. C., Antunes, L., Rangnekar, V. M., Nagai, M. A."Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells". International Journal of Oncology 43, no. 2 (2013): 531-538. https://doi.org/10.3892/ijo.2013.1983
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