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Article

Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells

  • Authors:
    • Xin Xu
    • Bin Wang
    • Yanling Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Shanghai No. 6 People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200233, P.R. China
  • Pages: 1578-1586
    |
    Published online on: August 21, 2013
       https://doi.org/10.3892/ijo.2013.2067
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Abstract

Lysyl oxidase (LOX) is an extracellular matrix (ECM) remodeling enzyme, which is involved in the development and progression of many types of tumors. LOX dysfunction is observed in colorectal, breast and ovarian cancer. However, the precise effects and molecular mechanisms of LOX action in osteosarcoma progression are still unknown. We evaluated the role of LOX in human osteosarcoma cell lines and clinical tumor samples in order to determine the function of this molecule. In our study, we showed that the expression level of LOX mRNA and protein were decreased in human osteosarcoma tissues as compared with normal tissue samples. In addition, we employed adenovirus-mediated overexpression of LOX in U-2OS and HOS cells to investigate the role of LOX in osteosarcoma cell lines. Adenovirus-mediated overexpression of LOX could efficiently increase the expression levels of LOX in osteosarcoma cell lines at both mRNA and protein levels. Increased expression of LOX inhibited the proliferation and migration of human osteosarcoma cells and promoted its apoptosis. Moreover, the Ki-67 and PCNA expression was decreased and MMP-2 and MMP-9 expression was inhibited. These findings also indicated that the effects of LOX may be mediated via the PI3K/AKT signaling pathway since LOX-mediated functions could be blocked by β-aminopropionitrile (β-APN), a LOX inhibitor. Taken together, our data indicated that LOX may be a tumor suppressor and could be regarded as a therapeutic target in human osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Xu X, Wang B and Xu Y: Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells. Int J Oncol 43: 1578-1586, 2013.
APA
Xu, X., Wang, B., & Xu, Y. (2013). Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells. International Journal of Oncology, 43, 1578-1586. https://doi.org/10.3892/ijo.2013.2067
MLA
Xu, X., Wang, B., Xu, Y."Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells". International Journal of Oncology 43.5 (2013): 1578-1586.
Chicago
Xu, X., Wang, B., Xu, Y."Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells". International Journal of Oncology 43, no. 5 (2013): 1578-1586. https://doi.org/10.3892/ijo.2013.2067
Copy and paste a formatted citation
x
Spandidos Publications style
Xu X, Wang B and Xu Y: Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells. Int J Oncol 43: 1578-1586, 2013.
APA
Xu, X., Wang, B., & Xu, Y. (2013). Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells. International Journal of Oncology, 43, 1578-1586. https://doi.org/10.3892/ijo.2013.2067
MLA
Xu, X., Wang, B., Xu, Y."Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells". International Journal of Oncology 43.5 (2013): 1578-1586.
Chicago
Xu, X., Wang, B., Xu, Y."Expression of lysyl oxidase in human osteosarcoma and its clinical significance: A tumor suppressive role of LOX in human osteosarcoma cells". International Journal of Oncology 43, no. 5 (2013): 1578-1586. https://doi.org/10.3892/ijo.2013.2067
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