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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2013 Volume 43 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

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Article

p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells

  • Authors:
    • Hirotsugu Notsuda
    • Akira Sakurada
    • Chiaki Endo
    • Yoshinori Okada
    • Akira Horii
    • Hiroshi Shima
    • Takashi Kondo
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan, Department of Molecular Pathology, Tohoku University School of Medicine, Sendai 980-8574, Japan, Division of Cancer Chemotherapy, Miyagi Cancer Center Research Institute, Natori 981-1293, Japan
  • Pages: 1569-1577
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/ijo.2013.2096
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Abstract

Overcoming acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKIs) is an emerging issue in lung cancer treatment. We report evidence that a GTPase-activating protein, p190-A RhoGAP (p190), is a potential molecular target for the treatment of lung adenocarcinoma. We documented inhibition of phosphorylation of p190 by EGFR-TKI treatment in lung adenocarcinoma cell lines. Small interfering RNA-mediated knockdown of p190 leads lung adenocarcinoma cells to growth suppression and to inhibition of invasion/migration through inducing cell cycle arrest but not apoptosis. These findings were observed not only in EGFR-TKI-sensitive cells but also in EGFR-TKI-resistant cells; even in cell lines harboring K-ras mutations. The mechanism of this inhibitory effect on growth and invasion/migration was Ras inactivation through disrupting the p190-A RhoGAP/p120RasGAP complex. In addition, a high level of p190 mRNA expression was observed in majority of surgically obtained tissue from lung adenocarcinoma patients. Overexpression of p190 mRNA associated with poor disease-free survival. The results suggest that overexpression of p190 mRNA may be involved in the carcinogenesis of lung adenocarcinoma. These findings indicate that p190 is a possible molecular target for treatment of lung adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Notsuda H, Sakurada A, Endo C, Okada Y, Horii A, Shima H and Kondo T: p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells . Int J Oncol 43: 1569-1577, 2013.
APA
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., & Kondo, T. (2013). p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells . International Journal of Oncology, 43, 1569-1577. https://doi.org/10.3892/ijo.2013.2096
MLA
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., Kondo, T."p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells ". International Journal of Oncology 43.5 (2013): 1569-1577.
Chicago
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., Kondo, T."p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells ". International Journal of Oncology 43, no. 5 (2013): 1569-1577. https://doi.org/10.3892/ijo.2013.2096
Copy and paste a formatted citation
x
Spandidos Publications style
Notsuda H, Sakurada A, Endo C, Okada Y, Horii A, Shima H and Kondo T: p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells . Int J Oncol 43: 1569-1577, 2013.
APA
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., & Kondo, T. (2013). p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells . International Journal of Oncology, 43, 1569-1577. https://doi.org/10.3892/ijo.2013.2096
MLA
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., Kondo, T."p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells ". International Journal of Oncology 43.5 (2013): 1569-1577.
Chicago
Notsuda, H., Sakurada, A., Endo, C., Okada, Y., Horii, A., Shima, H., Kondo, T."p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells ". International Journal of Oncology 43, no. 5 (2013): 1569-1577. https://doi.org/10.3892/ijo.2013.2096
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