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SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2

Retraction in: /10.3892/ijo.2023.5508
  • Authors:
    • Hongqi Li
    • Zhiqiang Feng
    • Weizhang Wu
    • Jing Li
    • Jinqian Zhang
    • Tingyi Xia
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Air Force General Hospital, Beijing 100142, P.R. China, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1420-1430
    |
    Published online on: September 16, 2013
       https://doi.org/10.3892/ijo.2013.2103
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Abstract

Lung cancer is the leading cause of death worldwide and associated with dismal prognoses. As a major mitochondrial deacetylase, SIRT3 regulates the activity of enzymes to coordinate global shifts in cellular metabolism and has important implications for tumor growth. Its role as a tumor suppressor or an oncogene in lung cancer is unclear, especially in non-small cell lung carcinoma (NSCLC). To identify the mechanism of SIRT3-interacting proteins, we performed a yeast two-hybrid screen using a human lung cDNA library. One of the positive clones encoded the full-length cDNA of the nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) gene and the interaction between SIRT3 and NMNAT2 was identified. The interaction on growth, proliferation, apoptosis of NSCLC cell lines, and energy metabolism related to SIRT3 were investigated. Screening from the library resulted in NMNAT2 gene. We found that NMNAT2 interacts with SIRT3 both in vitro and in vivo; SIRT3 binds to NMNAT2 deacetylating it. Downregulation of SIRT3 inhibited acetylation of NMNAT2 and NAD+ synthesis activity of the enzyme. Low expression of SIRT3 significantly inhibited mitotic entry, growth and proliferation of NSCLC cell lines and promoted apoptosis, which was related to energy metabolism involving in the interaction between SIRT3 and NMNAT2. Taken together, our results strongly suggest that the binding of SIRT3 with NMNAT2 is a novel regulator of cell proliferation and apoptosis in NSCLC cell lines, implicating the interaction between SIRT3 and NMNAT2, energy metabolism associated with SIRT3.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Feng Z, Wu W, Li J, Zhang J and Xia T: SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508. Int J Oncol 43: 1420-1430, 2013.
APA
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., & Xia, T. (2013). SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508. International Journal of Oncology, 43, 1420-1430. https://doi.org/10.3892/ijo.2013.2103
MLA
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., Xia, T."SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508". International Journal of Oncology 43.5 (2013): 1420-1430.
Chicago
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., Xia, T."SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508". International Journal of Oncology 43, no. 5 (2013): 1420-1430. https://doi.org/10.3892/ijo.2013.2103
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Feng Z, Wu W, Li J, Zhang J and Xia T: SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508. Int J Oncol 43: 1420-1430, 2013.
APA
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., & Xia, T. (2013). SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508. International Journal of Oncology, 43, 1420-1430. https://doi.org/10.3892/ijo.2013.2103
MLA
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., Xia, T."SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508". International Journal of Oncology 43.5 (2013): 1420-1430.
Chicago
Li, H., Feng, Z., Wu, W., Li, J., Zhang, J., Xia, T."SIRT3 regulates cell proliferation and apoptosis related to energy metabolism in non-small cell lung cancer cells through deacetylation of NMNAT2 Retraction in /10.3892/ijo.2023.5508". International Journal of Oncology 43, no. 5 (2013): 1420-1430. https://doi.org/10.3892/ijo.2013.2103
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