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Article

PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms

  • Authors:
    • Arianna Nicolussi
    • Sonia D'Inzeo
    • Gabriella Mincione
    • Amelia Buffone
    • Maria Carmela Di Marcantonio
    • Roberto Cotellese
    • Annadomenica Cichella
    • Carlo Capalbo
    • Cira Di Gioia
    • Francesco Nardi
    • Giuseppe Giannini
    • Anna Coppa
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy, Department of Experimental and Clinical Sciences, ‘G. d'Annunzio’ University Foundation, Chieti-Pescara, Italy , Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy, Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome, Italy
  • Pages: 548-556
    |
    Published online on: December 5, 2013
       https://doi.org/10.3892/ijo.2013.2208
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Abstract

Many clinical studies highlight the dichotomous role of PRDXs in human cancers, where they can exhibit strong tumor-suppressive or tumor-promoting functions. Recent evidence suggests that lower expression of PRDXs correlates with cancer progression in colorectal cancer (CRC) or in esophageal squamous carcinoma. In the thyroid, increased levels of PRDX1 has been described in follicular adenomas and carcinomas, as well as in thyroiditis, while reduced levels of PRDX6 has been found in follicular adenomas. We studied the expression of PRDX1 and PRDX6, in a series of thyroid tissue samples, covering different thyroid diseases, including 13 papillary thyroid carcinomas (PTCs). Our results show that PRDX1 and PRDX6 are significantly reduced in all PTCs compared to normal tissues, to non-neoplastic tissue (MNG) or follicular neoplasms. This reduction is strongly evident in PTCs harboring BRAF V600E (31% of our cases). Using TPC-1 and BCPAP and FRTL-5 cell lines, we demonstrate for the first time that the presence of BRAF V600E is responsible of the hypoexpression of PRDX1 and PRDX6 both at mRNA and protein levels. Finally, independently of BRAF status, we observe an interesting correlation between the tumor size, the presence of lymph node metastasis and the lowest PRDX1 and PRDX6 levels. Therefore, these data indicate that PRDX1 and PRDX6 expression not only may play a key role in papillary thyroid carcinogenesis via a BRAF V600E-dependent mechanism, but their determination could be considered as potential tumor marker for indicating tumor progression in PTCs, independently of BRAF status.
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Spandidos Publications style
Nicolussi A, D'Inzeo S, Mincione G, Buffone A, Di Marcantonio MC, Cotellese R, Cichella A, Capalbo C, Di Gioia C, Nardi F, Nardi F, et al: PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. Int J Oncol 44: 548-556, 2014.
APA
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M.C., Cotellese, R. ... Coppa, A. (2014). PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. International Journal of Oncology, 44, 548-556. https://doi.org/10.3892/ijo.2013.2208
MLA
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M. C., Cotellese, R., Cichella, A., Capalbo, C., Di Gioia, C., Nardi, F., Giannini, G., Coppa, A."PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms". International Journal of Oncology 44.2 (2014): 548-556.
Chicago
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M. C., Cotellese, R., Cichella, A., Capalbo, C., Di Gioia, C., Nardi, F., Giannini, G., Coppa, A."PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms". International Journal of Oncology 44, no. 2 (2014): 548-556. https://doi.org/10.3892/ijo.2013.2208
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Spandidos Publications style
Nicolussi A, D'Inzeo S, Mincione G, Buffone A, Di Marcantonio MC, Cotellese R, Cichella A, Capalbo C, Di Gioia C, Nardi F, Nardi F, et al: PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. Int J Oncol 44: 548-556, 2014.
APA
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M.C., Cotellese, R. ... Coppa, A. (2014). PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. International Journal of Oncology, 44, 548-556. https://doi.org/10.3892/ijo.2013.2208
MLA
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M. C., Cotellese, R., Cichella, A., Capalbo, C., Di Gioia, C., Nardi, F., Giannini, G., Coppa, A."PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms". International Journal of Oncology 44.2 (2014): 548-556.
Chicago
Nicolussi, A., D'Inzeo, S., Mincione, G., Buffone, A., Di Marcantonio, M. C., Cotellese, R., Cichella, A., Capalbo, C., Di Gioia, C., Nardi, F., Giannini, G., Coppa, A."PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms". International Journal of Oncology 44, no. 2 (2014): 548-556. https://doi.org/10.3892/ijo.2013.2208
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