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International Journal of Oncology
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2014-April Volume 44 Issue 4

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Article

The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM

  • Authors:
    • Liang Zeng
    • Chunsheng Kang
    • Chunhui Di
    • Brian E. Fee
    • Miriam Rivas
    • James Lin
    • David Cory Adamson
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, P.R. China, Preston Robert Tisch Brain Tumor Center, Departments of Surgery and Neurobiology, Duke University Medical Center, Durham, NC, USA, Durham VA Medical Center, Durham, NC, USA
  • Pages: 1243-1251
    |
    Published online on: January 24, 2014
       https://doi.org/10.3892/ijo.2014.2277
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Abstract

Previous studies identified the frequent loss of adherens junction-associated protein 1 (AJAP1) expression in glioblastoma (GBM) and its correlation with worse survival. AJAP1 may suppress glioma cell migration, which plays an important role in tumor progression in malignant gliomas such as GBM. However, the role of AJAP1 in cell cycle arrest or apoptosis and resistance to chemotherapy remains unclear. Based on microarray screening results, quantitative PCR and luciferase plasmid reporter constructs were used to evaluate the possible regulatory role of AJAP1 on MAGEA2 expression and function. Cell death assays, TUNEL and other markers of apoptosis were utilized to detect cell apoptosis. Restoration of AJAP1 expression in glioma cells was analyzed after temozolomide exposure. AJAP1 suppressed the expression of MAGEA2 and inhibited the transcriptional activity of MAGEA2 in glioma cells. As AJAP1 expression decreased MAGEA2 protein expression apoptosis increased moderately. Consistent with increased cell death, the induced loss of MAGEA2 expression correlated with increased caspase 3/7 activity, BCL2/BAX ratio and TUNEL signal. AJAP1 expression enhanced cell death in the presence of temozolomide. This study suggests AJAP1 may also function as a pro‑apoptotic factor and potentiate cell death by temozolomide in glioma cells. This effect may be partially explained by AJAP1‑mediated gene regulation of MAGEA2.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng L, Kang C, Di C, Fee BE, Rivas M, Lin J and Adamson DC: The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM. Int J Oncol 44: 1243-1251, 2014.
APA
Zeng, L., Kang, C., Di, C., Fee, B.E., Rivas, M., Lin, J., & Adamson, D.C. (2014). The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM. International Journal of Oncology, 44, 1243-1251. https://doi.org/10.3892/ijo.2014.2277
MLA
Zeng, L., Kang, C., Di, C., Fee, B. E., Rivas, M., Lin, J., Adamson, D. C."The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM". International Journal of Oncology 44.4 (2014): 1243-1251.
Chicago
Zeng, L., Kang, C., Di, C., Fee, B. E., Rivas, M., Lin, J., Adamson, D. C."The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM". International Journal of Oncology 44, no. 4 (2014): 1243-1251. https://doi.org/10.3892/ijo.2014.2277
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng L, Kang C, Di C, Fee BE, Rivas M, Lin J and Adamson DC: The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM. Int J Oncol 44: 1243-1251, 2014.
APA
Zeng, L., Kang, C., Di, C., Fee, B.E., Rivas, M., Lin, J., & Adamson, D.C. (2014). The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM. International Journal of Oncology, 44, 1243-1251. https://doi.org/10.3892/ijo.2014.2277
MLA
Zeng, L., Kang, C., Di, C., Fee, B. E., Rivas, M., Lin, J., Adamson, D. C."The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM". International Journal of Oncology 44.4 (2014): 1243-1251.
Chicago
Zeng, L., Kang, C., Di, C., Fee, B. E., Rivas, M., Lin, J., Adamson, D. C."The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM". International Journal of Oncology 44, no. 4 (2014): 1243-1251. https://doi.org/10.3892/ijo.2014.2277
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