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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-April Volume 44 Issue 4

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Article

Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater

  • Authors:
    • Nobuyasu Takahashi
    • Fumiyo Aoyama
    • Masahide Hiyoshi
    • Hiroaki Kataoka
    • Akira Sawaguchi
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan, Department of Surgical Oncology and Regulation of Organ Function, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan, Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan
  • Pages: 1139-1145
    |
    Published online on: January 28, 2014
       https://doi.org/10.3892/ijo.2014.2282
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Abstract

Hepatoid adenocarcinoma is a rare gastrointestinal tumor and mostly reported in the stomach. Effective chemotherapy has yet to be developed to improve poor prognosis. The present study was undertaken to establish a useful cell line derived from a hepatoid adenocarcinoma, possibly leading to a new therapeutic strategy. The new human cell line VAT-39 was established from a metastatic lymph node of a 69-year-old Japanese male patient with hepatoid adenocarcinoma of the ampulla of Vater. The primary tumor and metastatic lymph node were composed of hepatoid adenocarcinoma cells exhibiting immunohistochemical reactivity for alpha-fetoprotein (AFP) and glypican-3 (GPC3). In the metastatic lymph node, Periodic acid-Schiff (PAS) staining clarified diffuse deposition of glycogen in the cytoplasm, indicating analogous characteristics to the primary hepatoid adenocarcinoma. Moreover, VAT-39 cells produced high levels of AFP in the cultured medium, and reverse-transcriptase polymerase chain reaction (RT-PCR) verified increased expression of GPC3 mRNA in this cell line. Further, we evaluated the sensitivity to major chemotherapeutic drugs against the bile duct cancer. Neither 5-fluorouracil nor gemcitabine showed particular sensitivity to this cell line. The tumorigenicity of the cultured cells was confirmed in athymic nude mice and the histological features of the explanted tumor were similar to the VAT-39 cell line. The present VAT-39 is the first hepatoid adenocarcinoma cell line that originates from the ampulla of Vater and it will be applicable for basic biological studies searching for new strategies of molecular targeted chemotherapy to this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Takahashi N, Aoyama F, Hiyoshi M, Kataoka H and Sawaguchi A: Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater. Int J Oncol 44: 1139-1145, 2014.
APA
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., & Sawaguchi, A. (2014). Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater. International Journal of Oncology, 44, 1139-1145. https://doi.org/10.3892/ijo.2014.2282
MLA
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., Sawaguchi, A."Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater". International Journal of Oncology 44.4 (2014): 1139-1145.
Chicago
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., Sawaguchi, A."Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater". International Journal of Oncology 44, no. 4 (2014): 1139-1145. https://doi.org/10.3892/ijo.2014.2282
Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi N, Aoyama F, Hiyoshi M, Kataoka H and Sawaguchi A: Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater. Int J Oncol 44: 1139-1145, 2014.
APA
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., & Sawaguchi, A. (2014). Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater. International Journal of Oncology, 44, 1139-1145. https://doi.org/10.3892/ijo.2014.2282
MLA
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., Sawaguchi, A."Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater". International Journal of Oncology 44.4 (2014): 1139-1145.
Chicago
Takahashi, N., Aoyama, F., Hiyoshi, M., Kataoka, H., Sawaguchi, A."Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater". International Journal of Oncology 44, no. 4 (2014): 1139-1145. https://doi.org/10.3892/ijo.2014.2282
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