Id1 and NF-κB promote the generation of CD133+ and BMI-1+ keratinocytes and the growth of xenograft tumors in mice

Lai,Jinhuo; Cai,Qian; Biel,Merrill A.; Wang,Chuan; Hu,Xiaohua; Wang,Shaoyuan; Lin,Jizhen

doi:10.3892/ijo.2014.2309

Id1 and NF-κB promote the generation of CD133+ and BMI-1+ keratinocytes and the growth of xenograft tumors in mice

Authors:
- Jinhuo Lai
- Qian Cai
- Merrill A. Biel
- Chuan Wang
- Xiaohua Hu
- Shaoyuan Wang
- Jizhen Lin
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Affiliations: Department of Oncology of Union Hospital, Institute of Immunotherapy, Fujian Medical University, Fuzhou, P.R. China, Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangdong, P.R. China, Ear, Nose and Throat Specialty Care of Minnesota, Minneapolis, MN, USA
Published online on: February 21, 2014 https://doi.org/10.3892/ijo.2014.2309
Pages: 1481-1489
Copyright: © Lai et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Id1 and NF-κB are highly expressed in oral squamous cell carcinoma (OSCC). Whether they have a synergistic role in the carcinogenesis of OSCC is unclear. The current study was designed to demonstrate the synergy of both Id1 and NF-κB in the underlying disease mechanisms of OSCC using in vitro and in vivo animal models. Id1 and NF-κB strengthened the expression of both CD133 and BMI-1 in OSCC cell cultures. CD133+ and BMI-1+ keratinocytes from OSCC tissues and cell cultures initiated the growth of xenograft tumors in SCID/Beige mice. Id1 and NF-κB regulate the expression of CD133 and BMI-1 in an additive or synergistic manner in OSCC, which is associated with the generation of naïve and self-renewable keratinocytes and initiate the growth of xenograft tumors in vivo.

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