Open Access

Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation

  • Authors:
    • Jiaying Lin
    • Zhenzhong Deng
    • Chizu Tanikawa
    • Taro Shuin
    • Tsuneharu Miki
    • Koichi Matsuda
    • Yusuke Nakamura
  • View Affiliations

  • Published online on: February 27, 2014     https://doi.org/10.3892/ijo.2014.2314
  • Pages: 1490-1498
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In order to identify genes involved in renal carcinogenesis, we analyzed the expression profile of renal cell carcinomas (RCCs) using microarrays consisting of 27,648 cDNA or ESTs, and found a small heat shock protein, HSPB7, to be significantly and commonly downregulated in RCC. Subsequent quantitative PCR (qPCR) and immunohistochemical (IHC) analyses confirmed the downregulation of HSPB7 in RCC tissues and cancer cell lines in both transcriptional and protein levels. Bisulfite sequencing of a genomic region of HSPB7 detected DNA hypermethylation of some segments of HSPB7 in RCC cells and concordantly 5-aza-2'-deoxycytidine (5-Aza-dC) treatment of cancer cells restored HSPB7 expression significantly. Ectopic introduction of HSPB7 in five RCC cell lines remarkably suppressed cancer cell growth. Interestingly, we found that HSPB7 expression could be induced by p53 in a dose-dependent manner, indicating that this gene functions in the p53 pathway. Our results imply that HSBP7 is likely to be a tumor suppressor gene regulated by p53 and its downregulation by hypermethylation may play a critical role in renal carcinogenesis.
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May-2014
Volume 44 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lin J, Deng Z, Tanikawa C, Shuin T, Miki T, Matsuda K and Nakamura Y: Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation. Int J Oncol 44: 1490-1498, 2014
APA
Lin, J., Deng, Z., Tanikawa, C., Shuin, T., Miki, T., Matsuda, K., & Nakamura, Y. (2014). Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation. International Journal of Oncology, 44, 1490-1498. https://doi.org/10.3892/ijo.2014.2314
MLA
Lin, J., Deng, Z., Tanikawa, C., Shuin, T., Miki, T., Matsuda, K., Nakamura, Y."Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation". International Journal of Oncology 44.5 (2014): 1490-1498.
Chicago
Lin, J., Deng, Z., Tanikawa, C., Shuin, T., Miki, T., Matsuda, K., Nakamura, Y."Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation". International Journal of Oncology 44, no. 5 (2014): 1490-1498. https://doi.org/10.3892/ijo.2014.2314