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International Journal of Oncology
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Article

Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro

  • Authors:
    • Wei-Yi Gong
    • Jin-Feng Wu
    • Bao-Jun Liu
    • Hong-Ying Zhang
    • Yu-Xue Cao
    • Jing Sun
    • Yu-Bao Lv
    • Xiao Wu
    • Jing-Cheng Dong
  • View Affiliations / Copyright

    Affiliations: Institute of Integrative Medicine, Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200041, P.R. China
  • Pages: 1561-1570
    |
    Published online on: March 5, 2014
       https://doi.org/10.3892/ijo.2014.2320
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Abstract

The objective of the present study was to investigate the therapeutic efficacy of flavonoid components in Scutellaria baicalensis on proliferation, metastasis and lung cancer-associated inflammation during nicotine induction in the A549 and H1299 lung cancer cell lines. After experimental period, augmentation of proliferation was observed, accompanied by marked decrease in apoptotic cells in nicotine-induced lung cancer cells; additionally, nicotine-exposed cells exhibited increased invasive and migratory abilities based on invasion and wound-healing assay. Flavones in Scutellaria, baicalin, baicalein and wogonin significantly counteracted the above deleterious changes. Moreover, assessment of tumor apoptotic and metastatic factors on mRNA levels by quantitative PCR and protein levels by western blotting revealed that these phytochemical treatments effectively negated nicotine-induced upregulated expression of bcl-2, bcl-2/bax ratio, caspase-3, matrix metalloproteinase (MMP)-2 and MMP-9 as well as downregulated expression of bax. Further analysis of inflammatory markers such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 in cell culture supernatant and mRNA and protein expression of nuclear transcription factor-kappaB (NF-κB) and I kappa B-alpha (IκB-α) was carried out to substantiate the anti-inflammatory effect of flavones in Scutellaria in nicotine-exposed lung cancer cells. The therapeutic effects observed in the present study are attributed to the potent potential against proliferation, metastasis and inflammatory microenvironment by flavonoid components in Scutellaria in nicotine-induced lung cancer cells.
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View References

1. 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2. 

Chen W, Zhang S and Zou X: Estimation and projection of lung cancer incidence and mortality in China. Zhongguo Fei Ai Za Zhi. 13:488–493. 2010.(In Chinese).

3. 

Hecht SS: Cigarette smoking and lung cancer: chemical mechanisms and approaches to prevention. Lancet Oncol. 3:461–469. 2002. View Article : Google Scholar : PubMed/NCBI

4. 

Zhang J, Ou JX and Bai CX: Tobacco smoking in China: prevalence, disease burden, challenges and future strategies. Respirology. 16:1165–1172. 2011. View Article : Google Scholar : PubMed/NCBI

5. 

Egleton RD, Brown KC and Dasgupta P: Nicotinic acetylcholine receptors in cancer: multiple roles in proliferation and inhibition of apoptosis. Trends Pharmacol Sci. 29:151–158. 2008. View Article : Google Scholar : PubMed/NCBI

6. 

Thunnissen FB: Acetylcholine receptor pathway and lung cancer. J Thorac Oncol. 4:943–946. 2009. View Article : Google Scholar : PubMed/NCBI

7. 

Maneckjee R and Minna JD: Opioid and nicotine receptors affect growth regulation of human lung cancer cell lines. Proc Natl Acad Sci USA. 87:3294–3298. 1990. View Article : Google Scholar : PubMed/NCBI

8. 

Tournier JM and Birembaut P: Nicotinic acetylcholine receptors and predisposition to lung cancer. Curr Opin Oncol. 23:83–87. 2011. View Article : Google Scholar : PubMed/NCBI

9. 

Lam DC, Girard L, Ramirez R, et al: Expression of nicotinic acetylcholine receptor subunit genes in non-small-cell lung cancer reveals differences between smokers and nonsmokers. Cancer Res. 67:4638–4647. 2007. View Article : Google Scholar : PubMed/NCBI

10. 

Kelly PN and Strasser A: The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy. Cell Death Differ. 18:1414–1424. 2011. View Article : Google Scholar : PubMed/NCBI

11. 

Leber B, Geng F, Kale J and Andrews DW: Drugs targeting Bcl-2 family members as an emerging strategy in cancer. Expert Rev Mol Med. 12:e282010. View Article : Google Scholar : PubMed/NCBI

12. 

Xin M and Deng X: Nicotine inactivation of the proapoptotic function of Bax through phosphorylation. J Biol Chem. 280:10781–10789. 2005. View Article : Google Scholar : PubMed/NCBI

13. 

Dasgupta P, Rizwani W, Pillai S, et al: Nicotine induces cell proliferation, invasion and epithelial-mesenchymal transition in a variety of human cancer cell lines. Int J Cancer. 124:36–45. 2009. View Article : Google Scholar : PubMed/NCBI

14. 

Davis R, Rizwani W, Banerjee S, et al: Nicotine promotes tumor growth and metastasis in mouse models of lung cancer. PLoS One. 4:e75242009. View Article : Google Scholar : PubMed/NCBI

15. 

Nelson AR, Fingleton B, Rothenberg ML and Matrisian LM: Matrix metalloproteinases: biologic activity and clinical implications. J Clin Oncol. 18:1135–1149. 2000.PubMed/NCBI

16. 

Bayramoglu A, Gunes HV, Metintas M, Degirmenci I, Mutlu F and Alatas F: The association of MMP-9 enzyme activity, MMP-9 C1562T polymorphism, and MMP-2 and -9 and TIMP-1, -2, -3, and -4 gene expression in lung cancer. Genet Test Mol Biomarkers. 13:671–678. 2009. View Article : Google Scholar : PubMed/NCBI

17. 

Herbst RS, Yano S, Kuniyasu H, et al: Differential expression of E-cadherin and type IV collagenase genes predicts outcome in patients with stage I non-small cell lung carcinoma. Clin Cancer Res. 6:790–797. 2000.PubMed/NCBI

18. 

Klein G, Vellenga E, Fraaije MW, Kamps WA and de Bont ES: The possible role of matrix metalloproteinase (MMP)-2 and MMP-9 in cancer, e.g. acute leukemia. Crit Rev Oncol Hematol. 50:87–100. 2004. View Article : Google Scholar : PubMed/NCBI

19. 

Zong Y, Zhang ST and Zhu ST: Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide. World J Gastroenterol. 15:2500–2505. 2009. View Article : Google Scholar

20. 

Lee HZ, Leung HW, Lai MY and Wu CH: Baicalein induced cell cycle arrest and apoptosis in human lung squamous carcinoma CH27 cells. Anticancer Res. 25:959–964. 2005.PubMed/NCBI

21. 

Leung HW, Yang WH, Lai MY, Lin CJ and Lee HZ: Inhibition of 12-lipoxygenase during baicalein-induced human lung nonsmall carcinoma H460 cell apoptosis. Food Chem Toxicol. 45:403–411. 2007. View Article : Google Scholar : PubMed/NCBI

22. 

Du G, Han G, Zhang S, et al: Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1alpha inhibition. Eur J Pharmacol. 630:121–130. 2010. View Article : Google Scholar : PubMed/NCBI

23. 

Li-Weber M: New therapeutic aspects of flavones: the anti-cancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev. 35:57–68. 2009. View Article : Google Scholar : PubMed/NCBI

24. 

Lixuan Z, Jingcheng D, Wenqin Y, Jianhua H, Baojun L and Xiaotao F: Baicalin attenuates inflammation by inhibiting NF-kappaB activation in cigarette smoke induced inflammatory models. Pulm Pharmacol Ther. 23:411–419. 2010. View Article : Google Scholar : PubMed/NCBI

25. 

Li L, Bao H, Wu J, et al: Baicalin is anti-inflammatory in cigarette smoke-induced inflammatory models in vivo and in vitro: a possible role for HDAC2 activity. Int Immunopharmacol. 13:15–22. 2012. View Article : Google Scholar : PubMed/NCBI

26. 

Petty TL: Are COPD and lung cancer two manifestations of the same disease? Chest. 128:1895–1897. 2005. View Article : Google Scholar : PubMed/NCBI

27. 

Yang IA, Relan V, Wright CM, et al: Common pathogenic mechanisms and pathways in the development of COPD and lung cancer. Expert Opin Ther Targets. 15:439–456. 2011.PubMed/NCBI

28. 

Takahashi H, Ogata H, Nishigaki R, Broide DH and Karin M: Tobacco smoke promotes lung tumorigenesis by triggering IKKbeta- and JNK1-dependent inflammation. Cancer Cell. 17:89–97. 2010. View Article : Google Scholar

29. 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001.

30. 

Karunagaran D, Joseph J and Kumar TR: Cell growth regulation. Adv Exp Med Biol. 595:245–268. 2007. View Article : Google Scholar

31. 

Fennell DA: Caspase regulation in non-small cell lung cancer and its potential for therapeutic exploitation. Clin Cancer Res. 11:2097–2105. 2005. View Article : Google Scholar : PubMed/NCBI

32. 

Steeg PS: Tumor metastasis: mechanistic insights and clinical challenges. Nat Med. 12:895–904. 2006. View Article : Google Scholar : PubMed/NCBI

33. 

Chandrashekar N, Selvamani A, Subramanian R, Pandi A and Thiruvengadam D: Baicalein inhibits pulmonary carcinogenesis-associated inflammation and interferes with COX-2, MMP-2 and MMP-9 expressions in-vivo. Toxicol Appl Pharmacol. 261:10–21. 2012. View Article : Google Scholar : PubMed/NCBI

34. 

Tauler J and Mulshine JL: Lung cancer and inflammation: interaction of chemokines and hnRNPs. Curr Opin Pharmacol. 9:384–388. 2009. View Article : Google Scholar : PubMed/NCBI

35. 

Puliyappadamba VT, Cheriyan VT, Thulasidasan AK, et al: Nicotine-induced survival signaling in lung cancer cells is dependent on their p53 status while its downregulation by curcumin is independent. Mol Cancer. 9:2202010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gong W, Wu J, Liu B, Zhang H, Cao Y, Sun J, Lv Y, Wu X and Dong J: Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro. Int J Oncol 44: 1561-1570, 2014.
APA
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J. ... Dong, J. (2014). Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro. International Journal of Oncology, 44, 1561-1570. https://doi.org/10.3892/ijo.2014.2320
MLA
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J., Lv, Y., Wu, X., Dong, J."Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro". International Journal of Oncology 44.5 (2014): 1561-1570.
Chicago
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J., Lv, Y., Wu, X., Dong, J."Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro". International Journal of Oncology 44, no. 5 (2014): 1561-1570. https://doi.org/10.3892/ijo.2014.2320
Copy and paste a formatted citation
x
Spandidos Publications style
Gong W, Wu J, Liu B, Zhang H, Cao Y, Sun J, Lv Y, Wu X and Dong J: Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro. Int J Oncol 44: 1561-1570, 2014.
APA
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J. ... Dong, J. (2014). Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro. International Journal of Oncology, 44, 1561-1570. https://doi.org/10.3892/ijo.2014.2320
MLA
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J., Lv, Y., Wu, X., Dong, J."Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro". International Journal of Oncology 44.5 (2014): 1561-1570.
Chicago
Gong, W., Wu, J., Liu, B., Zhang, H., Cao, Y., Sun, J., Lv, Y., Wu, X., Dong, J."Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro". International Journal of Oncology 44, no. 5 (2014): 1561-1570. https://doi.org/10.3892/ijo.2014.2320
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