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Article

Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells

  • Authors:
    • Jeong-Mi Kim
    • Eun-Mi Noh
    • Mi-Seong Kim
    • Jin-Ki Hwang
    • Hong-Yeon Hwang
    • Do-Gon Ryu
    • Hye-Jung Kim
    • Hong-Nu Yu
    • Yong-Ouk You
    • Jong-Suk Kim
    • Hyun Jo Youn
    • Kang-Beom Kwon
    • Sung Hoo Jung
    • Young-Rae Lee
  • View Affiliations / Copyright

    Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea, Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea, Department of Korean Physiology, School of Korean Medicine, Wonkwang University, Iksan 570-749, Republic of Korea, Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, Bupyeong-gu, Incheon 403-720, Republic of Korea, Department of Medical Oncology, Xin Hua Hospital Affiliated to Dalian University, Dalian 116021, P.R. China, Department of Oral Biochemistry, and Institute of Biomaterials - Implant, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea, Division of Breast and Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea
  • Pages: 1607-1613
    |
    Published online on: March 5, 2014
       https://doi.org/10.3892/ijo.2014.2327
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Abstract

Decursin, a coumarin compound, was first isolated from the roots of Angelica gigas almost four decades ago. It was found to exhibit cytotoxicity against various human cancer cells and to possess anti-amnesic activity in vivo through the inhibition of AChE activity. However, the effect of decursin on breast cancer invasion is unknown. Matrix metalloproteinase-9 (MMP-9) is known to be an important factor for cancer cell invasion. Therefore, in this study, we investigated the inhibitory effect of decursin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. Our results showed that decursin inhibits TPA-induced MMP-9 expression and cell invasion through the suppression of NF-κB. Furthermore, decursin repressed the TPA-induced phosphorylation of p38 MAPK and inhibited TPA-induced translocation of PKCα from the cytosol to the membrane, but did not affect the translocation of PKCδ. These results indicate that decursin-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the PKCα, MAPK and NF-κB pathways in MCF-7 cells. Thus, decursin may have potential value in restricting breast cancer metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Noh E, Kim M, Hwang J, Hwang H, Ryu D, Kim H, Yu H, You Y, Kim J, Kim J, et al: Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. Int J Oncol 44: 1607-1613, 2014.
APA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D. ... Lee, Y. (2014). Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. International Journal of Oncology, 44, 1607-1613. https://doi.org/10.3892/ijo.2014.2327
MLA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44.5 (2014): 1607-1613.
Chicago
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44, no. 5 (2014): 1607-1613. https://doi.org/10.3892/ijo.2014.2327
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Noh E, Kim M, Hwang J, Hwang H, Ryu D, Kim H, Yu H, You Y, Kim J, Kim J, et al: Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. Int J Oncol 44: 1607-1613, 2014.
APA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D. ... Lee, Y. (2014). Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. International Journal of Oncology, 44, 1607-1613. https://doi.org/10.3892/ijo.2014.2327
MLA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44.5 (2014): 1607-1613.
Chicago
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44, no. 5 (2014): 1607-1613. https://doi.org/10.3892/ijo.2014.2327
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