Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells

  • Authors:
    • Jeong-Mi Kim
    • Eun-Mi Noh
    • Mi-Seong Kim
    • Jin-Ki Hwang
    • Hong-Yeon Hwang
    • Do-Gon Ryu
    • Hye-Jung Kim
    • Hong-Nu Yu
    • Yong-Ouk You
    • Jong-Suk Kim
    • Hyun Jo Youn
    • Kang-Beom Kwon
    • Sung Hoo Jung
    • Young-Rae Lee
  • View Affiliations

  • Published online on: March 5, 2014     https://doi.org/10.3892/ijo.2014.2327
  • Pages: 1607-1613
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Decursin, a coumarin compound, was first isolated from the roots of Angelica gigas almost four decades ago. It was found to exhibit cytotoxicity against various human cancer cells and to possess anti-amnesic activity in vivo through the inhibition of AChE activity. However, the effect of decursin on breast cancer invasion is unknown. Matrix metalloproteinase-9 (MMP-9) is known to be an important factor for cancer cell invasion. Therefore, in this study, we investigated the inhibitory effect of decursin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. Our results showed that decursin inhibits TPA-induced MMP-9 expression and cell invasion through the suppression of NF-κB. Furthermore, decursin repressed the TPA-induced phosphorylation of p38 MAPK and inhibited TPA-induced translocation of PKCα from the cytosol to the membrane, but did not affect the translocation of PKCδ. These results indicate that decursin-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the PKCα, MAPK and NF-κB pathways in MCF-7 cells. Thus, decursin may have potential value in restricting breast cancer metastasis.
View Figures
View References

Related Articles

Journal Cover

May-2014
Volume 44 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Noh E, Kim M, Hwang J, Hwang H, Ryu D, Kim H, Yu H, You Y, Kim J, Kim J, et al: Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. Int J Oncol 44: 1607-1613, 2014
APA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D. ... Lee, Y. (2014). Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells. International Journal of Oncology, 44, 1607-1613. https://doi.org/10.3892/ijo.2014.2327
MLA
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44.5 (2014): 1607-1613.
Chicago
Kim, J., Noh, E., Kim, M., Hwang, J., Hwang, H., Ryu, D., Kim, H., Yu, H., You, Y., Kim, J., Youn, H. J., Kwon, K., Jung, S. H., Lee, Y."Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells". International Journal of Oncology 44, no. 5 (2014): 1607-1613. https://doi.org/10.3892/ijo.2014.2327