Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells

Kim,Jeong-Mi; Noh,Eun-Mi; Kim,Mi-Seong; Hwang,Jin-Ki; Hwang,Hong-Yeon; Ryu,Do-Gon; Kim,Hye-Jung; Yu,Hong-Nu; You,Yong-Ouk; Kim,Jong-Suk; Youn,Hyun Jo; Kwon,Kang-Beom; Jung,Sung Hoo; Lee,Young-Rae

doi:10.3892/ijo.2014.2327

Decursin prevents TPA-induced invasion through suppression of PKCα/p38/NF-κB-dependent MMP-9 expression in MCF-7 human breast carcinoma cells

Authors:
- Jeong-Mi Kim
- Eun-Mi Noh
- Mi-Seong Kim
- Jin-Ki Hwang
- Hong-Yeon Hwang
- Do-Gon Ryu
- Hye-Jung Kim
- Hong-Nu Yu
- Yong-Ouk You
- Jong-Suk Kim
- Hyun Jo Youn
- Kang-Beom Kwon
- Sung Hoo Jung
- Young-Rae Lee
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Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea, Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea, Department of Korean Physiology, School of Korean Medicine, Wonkwang University, Iksan 570-749, Republic of Korea, Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, Bupyeong-gu, Incheon 403-720, Republic of Korea, Department of Medical Oncology, Xin Hua Hospital Affiliated to Dalian University, Dalian 116021, P.R. China, Department of Oral Biochemistry, and Institute of Biomaterials - Implant, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea, Division of Breast and Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea
Published online on: March 5, 2014 https://doi.org/10.3892/ijo.2014.2327
Pages: 1607-1613

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Abstract

Decursin, a coumarin compound, was first isolated from the roots of Angelica gigas almost four decades ago. It was found to exhibit cytotoxicity against various human cancer cells and to possess anti-amnesic activity in vivo through the inhibition of AChE activity. However, the effect of decursin on breast cancer invasion is unknown. Matrix metalloproteinase-9 (MMP-9) is known to be an important factor for cancer cell invasion. Therefore, in this study, we investigated the inhibitory effect of decursin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. Our results showed that decursin inhibits TPA-induced MMP-9 expression and cell invasion through the suppression of NF-κB. Furthermore, decursin repressed the TPA-induced phosphorylation of p38 MAPK and inhibited TPA-induced translocation of PKCα from the cytosol to the membrane, but did not affect the translocation of PKCδ. These results indicate that decursin-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the PKCα, MAPK and NF-κB pathways in MCF-7 cells. Thus, decursin may have potential value in restricting breast cancer metastasis.

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