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Article

Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels

  • Authors:
    • Bo Ra You
    • Woo Hyun Park
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Medical School, Research Institute for Endocrine Sciences, Chonbuk National University, Jeonju 561-180, Republic of Korea
  • Pages: 1745-1755
    |
    Published online on: March 12, 2014
       https://doi.org/10.3892/ijo.2014.2337
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Abstract

Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase (HDAC) inhibitor which has anticancer effects. We evaluated the growth inhibitory effects of SAHA on HeLa cervical cancer cells in relation to reactive oxygen species (ROS) levels. SAHA inhibited the growth of HeLa cells with an IC50 of approximately 10 µM at 24 h, and induced apoptosis which was accompanied by the cleavage of PARP, caspase-3 activation and loss of mitochondrial membrane potential (MMP; ∆ψm). All the tested caspase inhibitors prevented HeLa cell death induced by SAHA whereas TNF-α intensified apoptotic cell death in SAHA-treated HeLa cells. With respect to ROS and glutathione (GSH) levels, SAHA increased ROS levels, especially mitochondrial O2•- in HeLa cells and also induced GSH depletion. Caspase inhibitors reduced the levels of ROS and GSH depletion in SAHA-treated HeLa cells whereas TNF-α enhanced the levels in these cells. The well-known antioxidant N-acetyl cysteine (NAC) attenuated cell death and an increase in ROS levels was caused by SAHA. Moreover, SAHA decreased the levels of thioredoxin 1 (Trx1) in HeLa cells. While the downregulation of Trx1 enhanced cell death and ROS levels in SAHA-treated HeLa cells, the overexpression of Trx1 attenuated the levels in these cells. In conclusion, SAHA inhibited the growth of HeLa cell via caspase-dependent apoptosis, which was influenced by the mitochondrial O2•- and Trx1 levels.
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Copy and paste a formatted citation
Spandidos Publications style
You BR and Park WH: Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. Int J Oncol 44: 1745-1755, 2014.
APA
You, B.R., & Park, W.H. (2014). Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. International Journal of Oncology, 44, 1745-1755. https://doi.org/10.3892/ijo.2014.2337
MLA
You, B. R., Park, W. H."Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels". International Journal of Oncology 44.5 (2014): 1745-1755.
Chicago
You, B. R., Park, W. H."Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels". International Journal of Oncology 44, no. 5 (2014): 1745-1755. https://doi.org/10.3892/ijo.2014.2337
Copy and paste a formatted citation
x
Spandidos Publications style
You BR and Park WH: Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. Int J Oncol 44: 1745-1755, 2014.
APA
You, B.R., & Park, W.H. (2014). Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. International Journal of Oncology, 44, 1745-1755. https://doi.org/10.3892/ijo.2014.2337
MLA
You, B. R., Park, W. H."Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels". International Journal of Oncology 44.5 (2014): 1745-1755.
Chicago
You, B. R., Park, W. H."Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels". International Journal of Oncology 44, no. 5 (2014): 1745-1755. https://doi.org/10.3892/ijo.2014.2337
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