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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells

  • Authors:
    • Deok-Seon Ryu
    • Seon-Hee Kim
    • Ji-Hae Kwon
    • Dong-Seok Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Laboratory Science, Inje University, Gimhae, Gyungnam 621-749, Republic of Korea, Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Gyungnam 621-749, Republic of Korea
  • Pages: 459-469
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    Published online on: April 28, 2014
       https://doi.org/10.3892/ijo.2014.2404
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Abstract

We investigated the anticancer mechanisms of the ethylacetate (EtOAc) fraction from Orostachys japonicus in human gastric cancer (AGS) cells. Flow cytometric analysis revealed that the number of total apoptotic cells following treatment with the EtOAc fraction increased in a dose-dependent manner. In the cell cycle analyses, the EtOAc fraction increased the peak in the sub-G1, indicating apoptosis, and in the G2/M phases in a dose-dependent manner. In the RT-PCR analysis, the expression of cyclin-dependent kinase 1 (CDK 1) and cyclin B1 decreased in a dose- and time-dependent manner. The results of western blotting revealed that the protein levels of p53, cytochrome c, and cleaved caspase-3, -8 and -9 proteins increased and those of B cell lymphoma-2 (bcl-2) and pro-caspase-3, -8 and -9 proteins decreased in a dose- and time-dependent manner, whereas the levels of bcl-2-associated x protein (bax) remained unchanged. Furthermore, the changes in the levels of pro-caspase-3, -8 and -9 and cleaved caspase-3, -8 and -9 were abolished by the pan-caspase inhibitor Z-VAD-FMK. In addition, phosphorylation of p38 and JNK increased in a time-dependent manner. These results, for the first time, provide an understanding of the potential anticancer activity of the O. japonicus, which functions through the induction of apoptosis and cell cycle arrest.
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Copy and paste a formatted citation
Spandidos Publications style
Ryu D, Kim S, Kwon J and Lee D: Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells. Int J Oncol 45: 459-469, 2014.
APA
Ryu, D., Kim, S., Kwon, J., & Lee, D. (2014). Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells. International Journal of Oncology, 45, 459-469. https://doi.org/10.3892/ijo.2014.2404
MLA
Ryu, D., Kim, S., Kwon, J., Lee, D."Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells". International Journal of Oncology 45.1 (2014): 459-469.
Chicago
Ryu, D., Kim, S., Kwon, J., Lee, D."Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells". International Journal of Oncology 45, no. 1 (2014): 459-469. https://doi.org/10.3892/ijo.2014.2404
Copy and paste a formatted citation
x
Spandidos Publications style
Ryu D, Kim S, Kwon J and Lee D: Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells. Int J Oncol 45: 459-469, 2014.
APA
Ryu, D., Kim, S., Kwon, J., & Lee, D. (2014). Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells. International Journal of Oncology, 45, 459-469. https://doi.org/10.3892/ijo.2014.2404
MLA
Ryu, D., Kim, S., Kwon, J., Lee, D."Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells". International Journal of Oncology 45.1 (2014): 459-469.
Chicago
Ryu, D., Kim, S., Kwon, J., Lee, D."Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells". International Journal of Oncology 45, no. 1 (2014): 459-469. https://doi.org/10.3892/ijo.2014.2404
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