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International Journal of Oncology
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Article

Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders

  • Authors:
    • Piotr Smolewski
    • Magdalena Witkowska
    • Malgorzata Zwolinska
    • Barbara Cebula-Obrzut
    • Agata Majchrzak
    • Aleksandra Jeske
    • Zbigniew Darzynkiewicz
    • Wojciech Ardelt
    • Barbara Ardelt
    • Tadeusz Robak
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland, The Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA, TAmiR Biotechnology, Inc, Somerset, NJ, USA, Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland
  • Pages: 419-425
    |
    Published online on: April 28, 2014
       https://doi.org/10.3892/ijo.2014.2405
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Abstract

Although major advancements in antitumor treatment have been observed, several B cell-derived malignancies still remain incurable. A promising approach that involves targeting RNA either by the use of specific antisense oligonucleotides or cytostatic/cytotoxic ribonucleases (RNases) is being promoted. Two amphibian RNases, onconase (ONC; ranpirnase) and, more recently, r-amphinase (r-Amph), have already been tested, but thus far, mostly on solid tumors. In this study, for the first time we provide comprehensive data on ex vivo and in vivo cytotoxic activity of ONC or r-Amph against cancer cells from different B cell lymphoid malignancies, together with their detailed mode of antitumor action. Our data revealed strong pro-apoptotic activity of ONC and r-Amph in both chronic lymphocytic leukemia and aggressive B cell lymphomas, with less impact on acute lymphoblastic leukemia or multiple myeloma cells. Moreover, the antitumor action of ONC and r-Amph was markedly selective against neoplastic cells sparing normal, healthy control‑derived lymphocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T, Robak T, et al: Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol 45: 419-425, 2014.
APA
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A. ... Robak, T. (2014). Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. International Journal of Oncology, 45, 419-425. https://doi.org/10.3892/ijo.2014.2405
MLA
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A., Darzynkiewicz, Z., Ardelt, W., Ardelt, B., Robak, T."Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders". International Journal of Oncology 45.1 (2014): 419-425.
Chicago
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A., Darzynkiewicz, Z., Ardelt, W., Ardelt, B., Robak, T."Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders". International Journal of Oncology 45, no. 1 (2014): 419-425. https://doi.org/10.3892/ijo.2014.2405
Copy and paste a formatted citation
x
Spandidos Publications style
Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T, Robak T, et al: Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol 45: 419-425, 2014.
APA
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A. ... Robak, T. (2014). Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. International Journal of Oncology, 45, 419-425. https://doi.org/10.3892/ijo.2014.2405
MLA
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A., Darzynkiewicz, Z., Ardelt, W., Ardelt, B., Robak, T."Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders". International Journal of Oncology 45.1 (2014): 419-425.
Chicago
Smolewski, P., Witkowska, M., Zwolinska, M., Cebula-Obrzut, B., Majchrzak, A., Jeske, A., Darzynkiewicz, Z., Ardelt, W., Ardelt, B., Robak, T."Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders". International Journal of Oncology 45, no. 1 (2014): 419-425. https://doi.org/10.3892/ijo.2014.2405
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