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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1

  • Authors:
    • Xiaohong Tian
    • Weijian Hou
    • Shuling Bai
    • Jun Fan
    • Hao Tong
    • He Xu
  • View Affiliations / Copyright

    Affiliations: Department of Tissue Engineering, College of Basic Medical Sciences, China Medical University, Shenyang 110001, P.R. China
  • Pages: 121-128
    |
    Published online on: April 28, 2014
       https://doi.org/10.3892/ijo.2014.2406
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Abstract

Neuroblastoma (NB) is the most common extracranial solid tumor in childhood. One fundamental issue regarding NB recurrence and metastasis is the maintenance of cancer stem cells (CSCs) stemness. Tankyrase 1 (TNKS1) is overexpressed in several types of cancers and in NB cell lines. XAV939 is a small molecule inhibitor of TNKS1 and can induce apoptosis of NB cells. In this study, we showed that the surface marker CD133 method was more suitable for isolating NB CSCs than the side-population method, and 60 µM etoposide was optimal for enriching NB CSCs. The NB CSCs were demonstrated in juvenescence or stemness state by electron microscopy, which was in line with the characteristics of CSCs. Furthermore, we demonstrated that the expression of the CSCs marker CD133 and migration ability of CSCs decreased after XAV939 treatment or by RNAi‑mediated knockdown of the TNKS1 gene. These findings suggest that XAV939 treatment or RNAi-TNKS1 inhibits the stemness and migration of NB CSCs via the repression of TNKS1, and TNKS1 may be a potential molecular target for eliminating NB CSCs by small molecule drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Tian X, Hou W, Bai S, Fan J, Tong H and Xu H: XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1. Int J Oncol 45: 121-128, 2014.
APA
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., & Xu, H. (2014). XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1. International Journal of Oncology, 45, 121-128. https://doi.org/10.3892/ijo.2014.2406
MLA
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., Xu, H."XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1". International Journal of Oncology 45.1 (2014): 121-128.
Chicago
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., Xu, H."XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1". International Journal of Oncology 45, no. 1 (2014): 121-128. https://doi.org/10.3892/ijo.2014.2406
Copy and paste a formatted citation
x
Spandidos Publications style
Tian X, Hou W, Bai S, Fan J, Tong H and Xu H: XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1. Int J Oncol 45: 121-128, 2014.
APA
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., & Xu, H. (2014). XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1. International Journal of Oncology, 45, 121-128. https://doi.org/10.3892/ijo.2014.2406
MLA
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., Xu, H."XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1". International Journal of Oncology 45.1 (2014): 121-128.
Chicago
Tian, X., Hou, W., Bai, S., Fan, J., Tong, H., Xu, H."XAV939 inhibits the stemness and migration of neuroblastoma cancer stem cells via repression of tankyrase 1". International Journal of Oncology 45, no. 1 (2014): 121-128. https://doi.org/10.3892/ijo.2014.2406
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