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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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July-2014 Volume 45 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Regulatory role of nucleophosmin during the differentiation of human liver cancer cells

  • Authors:
    • Dong-Hui Xu
    • Fan Liu
    • Xiao Li
    • Xiang-Feng Chen
    • Guang-Jun Jing
    • Fu-Yun Wu
    • Song-Lin Shi
    • Qi-Fu Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatic Biliary Pancreatic Vascular Surgery, the First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China, Medical College of Xiamen University, Xiamen, Fujian 361102, P.R. China , School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, P.R. China
  • Pages: 264-272
    |
    Published online on: April 29, 2014
       https://doi.org/10.3892/ijo.2014.2407
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Abstract

Nucleophosmin (NPM, also known as B23), mainly localized in the nucleolus, has been reported to be overexpressed in many types of human cancer, including colon, ovarian, prostate and gastric cancer. NPM was identified while screening the differential nuclear matrix proteins during HMBA-induced differentiation of human liver cancer cells. We investigated the aberrant expression and subcellular localization of NPM in clinical liver cancer tissues and a cell line with the aim of providing more evidence for revealing the roles of NPM on regulating liver cancer cell proliferation and differentiation. In addition, we studied the potential interaction between NPM and several important proteins. Our results revealed that NPM protein was overexpressed in cancer cells, which was in accordance with the overexpressed mRNA in cancer tissues compared to the corresponding non-cancer tissues. We also found a decrease of NPM in protein and mRNA levels upon treatment with the differentiation reagent HMBA. We focused on the aberrant localization of NPM. Immunochemistry and immunofluorescence revealed aberrant cytoplasmic and nucleoplasm localization of NPM in liver cancer tissues and its colocalization with c-Myc, c-Fos, P53 and Rb in the SMMC-7721 cell line. The interactions between NPM and the above proteins were confirmed by GST pull-down assay and co-immunoprecipitation assay. These findings indicate that NPM plays a regulatory role in liver cancer, which deserves in-depth investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Xu D, Liu F, Li X, Chen X, Jing G, Wu F, Shi S and Li Q: Regulatory role of nucleophosmin during the differentiation of human liver cancer cells. Int J Oncol 45: 264-272, 2014.
APA
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F. ... Li, Q. (2014). Regulatory role of nucleophosmin during the differentiation of human liver cancer cells. International Journal of Oncology, 45, 264-272. https://doi.org/10.3892/ijo.2014.2407
MLA
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F., Shi, S., Li, Q."Regulatory role of nucleophosmin during the differentiation of human liver cancer cells". International Journal of Oncology 45.1 (2014): 264-272.
Chicago
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F., Shi, S., Li, Q."Regulatory role of nucleophosmin during the differentiation of human liver cancer cells". International Journal of Oncology 45, no. 1 (2014): 264-272. https://doi.org/10.3892/ijo.2014.2407
Copy and paste a formatted citation
x
Spandidos Publications style
Xu D, Liu F, Li X, Chen X, Jing G, Wu F, Shi S and Li Q: Regulatory role of nucleophosmin during the differentiation of human liver cancer cells. Int J Oncol 45: 264-272, 2014.
APA
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F. ... Li, Q. (2014). Regulatory role of nucleophosmin during the differentiation of human liver cancer cells. International Journal of Oncology, 45, 264-272. https://doi.org/10.3892/ijo.2014.2407
MLA
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F., Shi, S., Li, Q."Regulatory role of nucleophosmin during the differentiation of human liver cancer cells". International Journal of Oncology 45.1 (2014): 264-272.
Chicago
Xu, D., Liu, F., Li, X., Chen, X., Jing, G., Wu, F., Shi, S., Li, Q."Regulatory role of nucleophosmin during the differentiation of human liver cancer cells". International Journal of Oncology 45, no. 1 (2014): 264-272. https://doi.org/10.3892/ijo.2014.2407
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