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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway

  • Authors:
    • Sophie Huber
    • Sergio Valente
    • Patrick Chaimbault
    • Hervé Schohn
  • View Affiliations / Copyright

    Affiliations: University of Lorraine, CNRS Unit 7565 - SRSMC Campus Bridoux, F-57070 Metz, France, University of Lorraine, EA 4632 - Laboratory of Chemistry and Physics - A2MC, F-57078 Metz Cedex 3, France
  • Pages: 426-438
    |
    Published online on: April 29, 2014
       https://doi.org/10.3892/ijo.2014.2408
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Abstract

Thiazolidinediones have been shown to exhibit anti-proliferative effects against cancer cells derived from diverse tissue origins both in vivo and in vitro. We studied the anti-proliferative impact of 5-{4-(2-(5-ethyl-pyridin-2-yl)-ethoxy)-benzylidene}-thiazolidine-2,4-dione (∆2-pioglitazone), an analogue of pioglitazone, which binds to the nuclear peroxisome proliferator activated receptor-γ without activating it, on human adenocarcinoma-derived HT29 and HCT116 cells. In HTC116 cells, exposure to ∆2-pioglitazone reduced cell growth, but HT29 cells reached the plateau phase of growth after three days. ∆2-pioglitazone treatment did not trigger cells to enter apoptosis but enhanced the autophagy process. The effect of ∆2-pioglitazone treatment was related to the increase of oxygen and nitric oxide-derived species production and decreased glutathione content. Moreover, pre-treatment with an antioxidant before addition of ∆2-pioglitazone limited cell growth inhibition, reduced the production of reactive species and attenuated autophagy within the cells. The impact of the drug was associated with activation of the Nrf2/Keap1 pathway as demonstrated by the increased protein content of several antioxidant enzymes, notably heme-oxygenase-1.
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Copy and paste a formatted citation
Spandidos Publications style
Huber S, Valente S, Chaimbault P and Schohn H: Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway. Int J Oncol 45: 426-438, 2014.
APA
Huber, S., Valente, S., Chaimbault, P., & Schohn, H. (2014). Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway. International Journal of Oncology, 45, 426-438. https://doi.org/10.3892/ijo.2014.2408
MLA
Huber, S., Valente, S., Chaimbault, P., Schohn, H."Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway". International Journal of Oncology 45.1 (2014): 426-438.
Chicago
Huber, S., Valente, S., Chaimbault, P., Schohn, H."Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway". International Journal of Oncology 45, no. 1 (2014): 426-438. https://doi.org/10.3892/ijo.2014.2408
Copy and paste a formatted citation
x
Spandidos Publications style
Huber S, Valente S, Chaimbault P and Schohn H: Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway. Int J Oncol 45: 426-438, 2014.
APA
Huber, S., Valente, S., Chaimbault, P., & Schohn, H. (2014). Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway. International Journal of Oncology, 45, 426-438. https://doi.org/10.3892/ijo.2014.2408
MLA
Huber, S., Valente, S., Chaimbault, P., Schohn, H."Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway". International Journal of Oncology 45.1 (2014): 426-438.
Chicago
Huber, S., Valente, S., Chaimbault, P., Schohn, H."Evaluation of ∆2-pioglitazone, an analogue of pioglitazone, on colon cancer cell survival: Evidence of drug treatment association with autophagy and activation of the Nrf2/Keap1 pathway". International Journal of Oncology 45, no. 1 (2014): 426-438. https://doi.org/10.3892/ijo.2014.2408
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