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Article

Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis

  • Authors:
    • Takashi Fujitomo
    • Yataro Daigo
    • Koichi Matsuda
    • Koji Ueda
    • Yusuke Nakamura
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan, Laboratory for Biomarker Development, Center for Genomic Medicine, RIKEN, Yokohama 230-0045, Japan
  • Pages: 147-156
    |
    Published online on: May 6, 2014
       https://doi.org/10.3892/ijo.2014.2418
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Abstract

On the basis of the gene expression profiles of 120 lung cancer cases using a cDNA microarray containing 27,648 genes or expressed sequence tags (ESTs), we identified LRRC42 (Leucine-rich repeat containing 42) to be significantly upregulated in the majority of lung cancers. Northern blot analysis demonstrated that LRRC42 was expressed only in testis among normal tissues examined. Knockdown of LRRC42 expression by siRNA against LRRC42 significantly suppressed the growth of lung cancer cells. On the other hand, stable induction of LRRC42 expression significantly promoted cell growth. LRRC42, which was found to localize in the nucleus of mammalian cells, is likely to interact with and stabilize GATAD2B (GATA zinc finger domain-containing 2B) and MBD3 (Methyl-CpG-binding domain protein 3) proteins that could contribute to lung cancer cell proliferation partly through the regulation of p21Waf1/Cip1. Our findings suggest that LRRC42 overexpression as well as its interaction with LRRC42-GATAD2B might play essential roles in lung carcinogenesis, and be a promising molecular target for lung cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Fujitomo T, Daigo Y, Matsuda K, Ueda K and Nakamura Y: Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis. Int J Oncol 45: 147-156, 2014.
APA
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., & Nakamura, Y. (2014). Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis. International Journal of Oncology, 45, 147-156. https://doi.org/10.3892/ijo.2014.2418
MLA
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., Nakamura, Y."Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis". International Journal of Oncology 45.1 (2014): 147-156.
Chicago
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., Nakamura, Y."Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis". International Journal of Oncology 45, no. 1 (2014): 147-156. https://doi.org/10.3892/ijo.2014.2418
Copy and paste a formatted citation
x
Spandidos Publications style
Fujitomo T, Daigo Y, Matsuda K, Ueda K and Nakamura Y: Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis. Int J Oncol 45: 147-156, 2014.
APA
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., & Nakamura, Y. (2014). Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis. International Journal of Oncology, 45, 147-156. https://doi.org/10.3892/ijo.2014.2418
MLA
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., Nakamura, Y."Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis". International Journal of Oncology 45.1 (2014): 147-156.
Chicago
Fujitomo, T., Daigo, Y., Matsuda, K., Ueda, K., Nakamura, Y."Identification of a nuclear protein, LRRC42, involved in lung carcinogenesis". International Journal of Oncology 45, no. 1 (2014): 147-156. https://doi.org/10.3892/ijo.2014.2418
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