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Article

Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells

Corrigendum in: /10.3892/ijo.2015.2954
  • Authors:
    • Shigeo Koido
    • Sadamu Homma
    • Shin Kan
    • Kazuki Takakura
    • Yoshihisa Namiki
    • Hiroko Kobayashi
    • Zensho Ito
    • Kan Uchiyama
    • Mikio Kajihara
    • Seiji Arihiro
    • Hiroshi Arakawa
    • Masato Okamoto
    • Toshifumi Ohkusa
    • Jianlin Gong
    • Hisao Tajiri
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine, Chiba 277-8564, Japan, Department of Oncology, Jikei University School of Medicine, Tokyo 105-8461, Japan, Institute of Clinical Medicine and Research, Jikei University School of Medicine, Chiba 277-8564, Japan, Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo 160-8582, Japan, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
  • Pages: 470-478
    |
    Published online on: May 9, 2014
       https://doi.org/10.3892/ijo.2014.2433
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Abstract

Previous work has demonstrated that fusion cells generated from autologous monocyte-derived dendritic cells (MoDCs) and whole tumor cells induce efficient antigen-specific cytotoxic T lymphocytes. A major limitation to the use of this strategy is the availability of adequate amounts of autologous tumor cells. Moreover, MoDCs from cancer patients are often defective in their antigen-processing and presentation machinery. In this study, two types of allogeneic cells, a leukemia plasmacytoid dendritic cell (pDC) line (PMDC05) and pancreatic cancer cell lines (PANC-1 or MIA PaCa-2), were fused instead of autologous MoDCs and tumor cells. We created four types of pDC/tumor fusion cells by alternating fusion partners and treating with lipopolysaccharide (LPS): i) PMDC05 fused with PANC-1 (pDC/PANC-1), ii) PMDC05 fused with MIA PaCa-2 (pDC/MIA PaCa-2), iii) LPS-stimulated pDC/PANC-1 (LPS-pDC/PANC-1) and iv) LPS-stimulated pDC/MIA PaCa-2 (LPS-pDC/MIA PaCa-2) and examined their antitumor immune responses. The LPS-pDC/tumor cell fusions were the most active, as demonstrated by their: i) upregulated expression of HLA-DR and CD86 on a per-fusion-cell basis, ii) increased production of IL-12p70, iii) generation of a higher percentage of IFN-γ-producing CD4+ and CD8+ T cells and iv) augmented induction of MUC1-specific CD8+ T cells that lyse target tumor cells. This study provides the first evidence for an in vitro induction of antigen-specific cytotoxic T lymphocytes by LPS-stimulated fusion cells generated from leukemia plasmacytoid DCs and tumor cells and suggests that this strategy has potential applicability to the field of adoptive immunotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Koido S, Homma S, Kan S, Takakura K, Namiki Y, Kobayashi H, Ito Z, Uchiyama K, Kajihara M, Arihiro S, Arihiro S, et al: Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954. Int J Oncol 45: 470-478, 2014.
APA
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H. ... Tajiri, H. (2014). Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954. International Journal of Oncology, 45, 470-478. https://doi.org/10.3892/ijo.2014.2433
MLA
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H., Ito, Z., Uchiyama, K., Kajihara, M., Arihiro, S., Arakawa, H., Okamoto, M., Ohkusa, T., Gong, J., Tajiri, H."Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954". International Journal of Oncology 45.1 (2014): 470-478.
Chicago
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H., Ito, Z., Uchiyama, K., Kajihara, M., Arihiro, S., Arakawa, H., Okamoto, M., Ohkusa, T., Gong, J., Tajiri, H."Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954". International Journal of Oncology 45, no. 1 (2014): 470-478. https://doi.org/10.3892/ijo.2014.2433
Copy and paste a formatted citation
x
Spandidos Publications style
Koido S, Homma S, Kan S, Takakura K, Namiki Y, Kobayashi H, Ito Z, Uchiyama K, Kajihara M, Arihiro S, Arihiro S, et al: Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954. Int J Oncol 45: 470-478, 2014.
APA
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H. ... Tajiri, H. (2014). Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954. International Journal of Oncology, 45, 470-478. https://doi.org/10.3892/ijo.2014.2433
MLA
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H., Ito, Z., Uchiyama, K., Kajihara, M., Arihiro, S., Arakawa, H., Okamoto, M., Ohkusa, T., Gong, J., Tajiri, H."Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954". International Journal of Oncology 45.1 (2014): 470-478.
Chicago
Koido, S., Homma, S., Kan, S., Takakura, K., Namiki, Y., Kobayashi, H., Ito, Z., Uchiyama, K., Kajihara, M., Arihiro, S., Arakawa, H., Okamoto, M., Ohkusa, T., Gong, J., Tajiri, H."Induction of antigen-specific cytotoxic T lymphocytes by fusion cells generated from allogeneic plasmacytoid dendritic and tumor cells Corrigendum in /10.3892/ijo.2015.2954". International Journal of Oncology 45, no. 1 (2014): 470-478. https://doi.org/10.3892/ijo.2014.2433
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