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Article

Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements

  • Authors:
    • Alaina M. Forbes
    • Huimin Lin
    • Gary G. Meadows
    • G. Patrick Meier
  • View Affiliations / Copyright

    Affiliations: Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA, Department of Pharmaceutical Sciences, Washington State University, Pullman, WA 99164-4630, USA
  • Pages: 831-842
    |
    Published online on: May 21, 2014
       https://doi.org/10.3892/ijo.2014.2452
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Abstract

Flavonoids have been studied intensely for their ability to act as anti-carcinogenic, anti-inflammatory, anti-viral and anti-aging agents and are often marketed as supplements related to their anti-inflammatory activity. Previous studies have primarily focused on the effects of polar natural flavonoids. We examined the activity of novel hydrophobic and lipophilic flavonols against human DU-145 and PC-3 prostate cancer cell lines. All flavonol analogs were more active than the naturally occurring flavonols quercetin, kaempferol, kaempferide and galangin. The most potent analogs were 6.5-fold more active against DU-145 and PC-3 cells than quercetin and fell within the biologically relevant concentration range (low micromolar). We also evaluated the potential toxic effects of flavonol analogs on normal cells, an assessment that has frequently been ignored when studying the anticancer effects of flavonoids. During these analyses, we discovered that various metabolic and DNA staining assays were unreliable methods for assessing cell viability of flavonoids. Flavonoids reduce colorimetric dyes such as MTT and Alamar Blue in the absence of cells. We showed that flavonol-treated prostate cancer cells were stained less intensely with crystal violet than untreated cells at non-toxic concentrations. The trypan blue exclusion assay was selected as a reliable alternative for measuring cell viability.
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1 

Dorta DJ, Curti C and Rodrigues T: Effects of flavonoids on mitochondria: An overview on pharmacological and toxicological aspects. Mitochondrial Pharmacology and Toxicology. Moreno AJM, Oliveira PJ and Palmeira CM: Transworld Research Network; Kerala, India: pp. 147–161. 2006

2 

Agullo G, Gamet-Payrastre L, Manenti S, Viala C, Remesy C, Chap H and Payrastre B: Relationship between flavonoid structure and inhibition of phosphatidylinositol 3-kinase: A comparison with tyrosine kinase and protein kinase C inhibition. Biochem Pharmacol. 53:1649–1657. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Haddad AQ, Venkateswaran V, Viswanathan L, Teahan SJ, Fleshner NE and Klotz LH: Novel antiproliferative flavonoids induce cell cycle arrest in human prostate cancer cell lines. Prostate Cancer Prostatic Dis. 9:68–76. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Nair HK, Rao KV, Aalinkeel R, Mahajan S, Chawda R and Schwartz SA: Inhibition of prostate cancer cell colony formation by flavonoid quercetin correlates with modulation of specific regulatory genes. Clin Diagn Lab Immunol. 11:63–69. 2004.PubMed/NCBI

5 

Mokhtari MJ, Motamed N and Shokrgozar MA: Evaluation of silibinin on the viability, migration, and adhesion of the human prostate adenocarcinoma (PC-3) cell line. Cell Bio Int. 32:888–892. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Daskiewicz JB, Depeint F, Viornery L, et al: Effects of flavonoids on cell proliferation and caspase activation in human colonic cell line HT29: an SAR study. J Med Chem. 48:2790–2804. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Knowles LM, Zigrossi DA, Tauber RA, Hightower C and Milner JA: Flavonoids suppress androgen-independent human prostate tumor proliferation. Nutr Cancer. 38:116–122. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Skibola CF and Smith MT: Potential health impacts of excessive flavonoid intake. Free Radic Biol Med. 29:375–383. 2000. View Article : Google Scholar : PubMed/NCBI

9 

MacGregor JT and Jurd L: Mutagenicity of plant flavonoids: structural requirements for mutagenic activity in Salmonella typhimurium. Mutat Res. 54:297–309. 1978. View Article : Google Scholar : PubMed/NCBI

10 

Carver JH, Carrano AV and MacGregor JT: Genetic effects of the flavonols quercetin, kaempferol, and galangin on Chinese hamster ovary cells in vitro. Mutat Res. 113:45–60. 1983. View Article : Google Scholar : PubMed/NCBI

11 

Bjeldanes LF and Chang GW: Mutagenic activity of quercetin and related compounds. Science. 197:577–578. 1977. View Article : Google Scholar : PubMed/NCBI

12 

Brown JP and Dietrich PS: Mutagenicity of plant flavonols in Salmonella/mammalian microsome test: activation of flavonol glycosides by mixed glycosidases from rat cecal bacteria and other sources. Mutat Res. 66:223–240. 1979.PubMed/NCBI

13 

Hillard JJ, Krause HM, Brenstein JI, Fernandez JA, Nguyen V, Ohemeng KA and Barrett JF: A comparison of active binding of 4-quinolones and novel flavone gyrase inhibitors to DNA gyrase. Adv Exp Med Biol. 390:59–69. 1995.PubMed/NCBI

14 

Guthrie N and Manthey JA: Antiproliferative activities of citrus flavonoids against six human cancer cell lines. J Agric Food Chem. 50:5837–5843. 2002. View Article : Google Scholar : PubMed/NCBI

15 

García-Mediavilla V, Crespo I, Collado PS, Esteller A, Sánchez-Campos S, Tuñón MJ and González-Gallego J: The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2, and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells. Eur J Pharmacol. 557:221–229. 2007.

16 

Kaushik D, O’Fallon K, Clarkson PM, Dunne CP, Conca KR and Michniak-Kohn B: Comparison of quercetin pharmacokinetics following oral supplementation in humans. J Food Sci. 77:H231–H238. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Suomela JP, Ahotupa M, Yang B, Vasankari T and Kallio H: Adsorption of flavonols derived from sea buckthorn (Hippophae rhamnoides L.) and their effects on emerging risk factors for cardiovascular disease in humans. J Agric Food Chem. 54:7364–7369. 2006.PubMed/NCBI

18 

Manach C, Williamson G, Morand C, Scalbert A and Rémésy C: Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 81(Suppl 1): S230–S242. 2005.PubMed/NCBI

19 

Del Rio DD, Borges G and Crozier A: Berry flavonoids and phenolics: bioavailability and evidence of protective effects. Br J Nutr. 104(Suppl 3): S67–S90. 2010.PubMed/NCBI

20 

Gao S and Hu M: Bioavailability challenges associated with development of anti-cancer phenolics. Mini Rev Med Chem. 10:550–567. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Singh B, Mense SM, Bhat NK, Putty S, Guthiel WA, Remotti F and Bhat HK: Dietary quercetin exacerbates the development of estrogen-induced breast tumors in female ACI rats. Toxicol Appl Pharmacol. 247:83–90. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Yadegarynia S, Pham A, Ng A, et al: Profiling flavonoid cytotoxicity in human breast cancer cell lines: determination of structure-function relationships. Nat Prod Commun. 7:1295–1304. 2012.PubMed/NCBI

23 

Chang H, Mi M, Ling W, et al: Structurally related cytotoxic effects of flavonoids on human cancer cells in vitro. Arch Pharm Res. 31:1137–1144. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Li X, Wang X, Ye H, Peng A and Chen L: Barbigerone, an isoflavone, inhibits tumor angiogenesis and human non-small-cell lung cancer xenografts growth through VEGFR2 signaling pathways. Cancer Chemother Pharmacol. 70:425–437. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Wu TC, Yang YC, Huang PR, Wen YD and Yeh SL: Genistein enhances the effect of trichostatin A on inhibition of A549 cell growth by increasing expression of TNF receptor-1. Toxicol Appl Pharmacol. 262:247–254. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Wesolowska O, Wiśniewski J, Sroda-Pomianek K, et al: Multidrug resistance reversal and apoptosis induction in human colon cancer cells by some flavonoids present in citrus plants. J Nat Prod. 75:1896–1902. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Vaid M, Prasad R, Singh T, Jones V and Katiyar SK: Grape seed proanthocyanidins reactivate silenced tumor suppressor genes in human skin cancer cells by targeting epigenetic regulators. Toxicol Appl Pharmacol. 263:122–130. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Lee DH, Szczepanski M and Lee YJ: Role of Bax in quercetin-induced apoptosis in human prostate cancer cells. Biochem Pharmacol. 75:2345–2355. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Gamet-Payrastre L, Manenti S, Gratacap MP, Tulliez J, Chap H and Payrastre B: Flavonoids and the inhibition of PKC and PI 3-Kinase. Gen Pharmacol. 32:279–286. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Lee LT, Huang YT, Hwang JJ, et al: Transinactivation of the epidermal growth factor receptor tyrosine kinase and focal adhesion kinase phosphorylation by dietary flavonoids; effect on invasive potential of human carcinoma cells. Biochem Pharmacol. 67:2103–2114. 2004. View Article : Google Scholar

31 

Di Pietro A, Conseil G, Perez-Victoria JM, et al: Modulation by flavonoids of cell multidrug resistance mediated by P-glycoprotein and related ABC transporters. Cell Mol Life Sci. 59:307–322. 2002.PubMed/NCBI

32 

Politzer P, Lane P, Concha MC, Ma Y and Murray JS: An overview of halogen bonding. J Mol Model. 13:305–311. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Bruggisser R, von Daeniken K, Jundt G, Schaffner W and Tullberg-Reinert H: Interference of plant extracts, phytoestrogens, and antioxidants with MTT tetrazolium assay. Planta Med. 68:445–448. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Molander GA and Biolatto B: Palladium-catalyzed Suzuki-Miyaura cross-coupling reactions of potassium aryl- and heteroaryltrifluoroborates. J Org Chem. 68:4302–4314. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Khan N, Afaq F, Syed DN and Mukhtar H: Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cells. Carcinogenesis. 29:1049–1056. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Harborne JB, Grayer RF, Porter LF, et al: The Flavonoids Advances in Research Since 1980. Harborne, New York: pp. 399–420. 1988

37 

Tanaka H, Stohlmeyer MM, Wandless TJ and Taylor LP: Synthesis of flavonol derivatives as probes of biological processes. Tetrahedron Lett. 41:9735–9739. 2000. View Article : Google Scholar

38 

Ichikawa M, Pamukcu AM and Bryan GT: A convenient method for the synthesis of kaempferol. Org Prep Proced Int. 14:183–187. 1982. View Article : Google Scholar

39 

Urgaonkar S and Shaw JT: Synthesis of kaempferitrin. J Org Chem. 72:4582–4585. 2007. View Article : Google Scholar : PubMed/NCBI

40 

Landini D, Montanari F and Rolla F: Cleavage of dialkyl and aryl alkyl ethers with hydrobromic acid in the presence of phase-transfer catalysts. Synthesis. 10:771–773. 1978. View Article : Google Scholar

41 

Boumendjel A, Bois F, Beney C, Mariotte AM, Conseil G and Di Pietro A: B-ring substituted 5,7-dihydroxyflavonols with high-affinity binding to P-glycoprotein responsible for cell multidrug resistance. Bioorg Med Chem Let. 11:75–77. 2001. View Article : Google Scholar : PubMed/NCBI

42 

Azimova SS and Vinogradova VIG: Galangin. Natural Compounds: Flavonoids: Plant Sources, Structure and Properties. Springer; New York: pp. 962013

43 

Batirov EK, Kiyamitdinova F and Malikov VM: Flavonoids of the epigeal part of Glycyrrhiza glabra. Chem Nat Compd. 22:107–108. 1986. View Article : Google Scholar

44 

Yuldashev MP: Flavonoids of the epigeal part of Glycyrrhiza uralensis. Chem Nat Compd. 34:508–509. 1998. View Article : Google Scholar

45 

Agrawal PK and Rastogi RP: 13C NMR spectroscopy of flavonoids. Heterocycles. 16:21811981. View Article : Google Scholar

46 

Xu R, Zhang Y, Ye X, Xue S, Shi J, Pan J and Chen Q: Inhibition effects and induction of apoptosis of flavonoids on the prostate cancer cell line PC-3 in vitro. Food Chem. 138:48–53. 2013. View Article : Google Scholar : PubMed/NCBI

47 

Torkin R, Lavoie JF, Kaplan DR and Yeger H: Induction of caspase-dependent, p53-mediated apoptosis by apigenin in human neuroblastoma. Mol Cancer Ther. 4:1–11. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Ferguson PJ, Kurowska E, Freeman DJ, Chambers AF and Koropatnick DJ: A flavonoid fraction from cranberry extract inhibits proliferation of human tumor cell lines. J Nutr. 134:1529–1535. 2004.PubMed/NCBI

49 

Walle T, Ta N, Kawamori T, Wen X, Tsuji PA and Walle UK: Cancer chemopreventive properties of orally bioavailable flavonoids - methylated versus unmethylated flavones. Biochem Pharmacol. 73:1288–1296. 2007. View Article : Google Scholar

50 

Gosslau A, Chen M, Ho CT and Chen KY: A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblast. Br J Cancer. 92:513–521. 2005.

51 

Berridge MV and Tan AS: Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys. 303:474–482. 1993. View Article : Google Scholar

52 

Dunigan DD, Waters SB and Owen TC: Aqueous soluble tetrazolium/formazan MTS as an indicator of NADH- and NADPH-dependent dehydrogenase activity. Biotechniques. 19:640–649. 1995.PubMed/NCBI

53 

Maeda H, Matsu-Ura S, Yamauchi Y and Ohmori H: Resaurin as an electron acceptor in glucose oxidase-catalyzed oxidation of glucose. Chem Pharm Bull. 49:622–625. 2001. View Article : Google Scholar : PubMed/NCBI

54 

Suszynski TM, Wildey GM, Falde EJ, et al: The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. Transplant Proc. 40:346–350. 2008. View Article : Google Scholar : PubMed/NCBI

55 

Wisman KN, Perkins AA, Jeffers MD and Hagerman AE: Accurate assessment of the bioactivities of redox-active polyphenolics in cell culture. J Agric Food Chem. 56:7831–7837. 2008. View Article : Google Scholar : PubMed/NCBI

56 

Maioli E, Torricelli C, Fortino V, Carlucci F, Tommassini V and Pacini A: Critical appraisal of the MTT assay in the presence of rottlerin and uncouplers. Biol Proced Online. 11:227–240. 2009. View Article : Google Scholar : PubMed/NCBI

57 

Wang P, Henning SM and Heber D: Limitations of MTT and MTS-based assays for measurement of antiproliferative activity of green tea polyphenols. PLoS One. 5:e102022010. View Article : Google Scholar : PubMed/NCBI

58 

Zhang D, Liu Y, Chu L, et al: Relationship between the structures of flavonoids and oxygen radical absorbance capacity values: a quantum chemical analysis. J Phys Chem A. 117:1784–1794. 2013. View Article : Google Scholar : PubMed/NCBI

59 

Pourcel L, Routaboul JM, Cheynier V, Lepiniec L and Debeaujon I: Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci. 12:29–36. 2007. View Article : Google Scholar : PubMed/NCBI

60 

Jacobs H, Moalin M, Bast A, van der Vijgh WJ and Haenen GR: An essential difference between the flavonoids monoHER and quercetin in their interplay with the endogenous antioxidant network. PLoS One. 5:e138802010. View Article : Google Scholar : PubMed/NCBI

61 

Wang S, Yu H and Wickliffe JK: Limitations of the MTT and XTT assays for measuring cell viability due to superoxide formation induced by nano-scale TiO2. Toxicol In Vitro. 25:2147–2151. 2011. View Article : Google Scholar : PubMed/NCBI

62 

Stockert JC, Blázquez-Castro A, Cañete M, Horobin RW and Villanueva A: MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. Acta Histochem. 114:785–796. 2012. View Article : Google Scholar : PubMed/NCBI

63 

Tranzer JP and Pearse AG: Cytochemical demonstration of ubiquinones in animal tissues. Nature. 199:1063–1066. 1963. View Article : Google Scholar : PubMed/NCBI

64 

Carmichael J, DeGraff WG, Gazdar AF, Minna JD and Mitchell JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47:936–942. 1987.PubMed/NCBI

65 

Shapiro AB and Ling V: Positively cooperative sites for drug transport by P-glycoprotein with distinct drug specificities. Eur J Biochem. 250:130–137. 1997. View Article : Google Scholar : PubMed/NCBI

66 

Bennett MD, Price HJ and Johnston JS: Anthocyanin inhibits propidium iodide DNA fluorescence in Euphorbia pulcherrima: implications for genome size variation and flow cytometry. Ann Bot. 101:777–790. 2008. View Article : Google Scholar : PubMed/NCBI

67 

Tajmir-Riahi HA, Diamantoglou S, Kanakis CD, Tarantilis PA and Polissiou MG: Flavonoids interactions with DNA and RNA: binding modes and antioxidative effects. Acta Hort. 744:195–204. 2007.

68 

Baracca A, Sqarbi G, Solaini G and Lenaz G: Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis. Biochim Biophys Acta. 1606:137–146. 2003. View Article : Google Scholar : PubMed/NCBI

69 

Shapiro AB and Ling V: Effect of quercetin on Hoechst 33342 transport by purified and reconstituted P-glycoprotein. Biochem Pharmacol. 53:587–596. 1997. View Article : Google Scholar : PubMed/NCBI

70 

Liu Y, Peterson DA, Kimura H and Schubert D: Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. J Neurochem. 69:581–593. 1997. View Article : Google Scholar

71 

Altman SA, Randers L and Rao G: Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnol Prog. 9:671–674. 1993. View Article : Google Scholar : PubMed/NCBI

72 

Smith AL and Smith HV: A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability. Parasitology. 99:329–331. 1989. View Article : Google Scholar : PubMed/NCBI

73 

Mascotti K, McCullough J and Burger SR: HPC viability measurement: trypan blue versus acridine orange and propidium iodide. Transfusion. 40:693–696. 2000. View Article : Google Scholar : PubMed/NCBI

74 

Szabo SE, Monroe SL, Fiorino S, Bitzan J and Loper K: Evaluation of an automated instrument for viability and concentration measurements of cryopreserved hemapoietic cells. Lab Hematol. 10:109–111. 2004. View Article : Google Scholar : PubMed/NCBI

75 

Vijayababu MR, Arunkumar A, Kanagaraj P, Venkataraman P, Krishnamoorthy G and Arunakaran J: Quercetin downregulates matrix metalloproteinases 2 and 9 proteins in prostate cancer (PC-3). Mol Cell Biochem. 287:109–116. 2006. View Article : Google Scholar : PubMed/NCBI

76 

Hollman PC and Katan MB: Absorption, metabolism and health effects of dietary flavonoids in man. Biomed Pharmacother. 51:305–310. 1997. View Article : Google Scholar : PubMed/NCBI

77 

Hollman PC, van Trijp JM, Mengelers MJ, de Vries JH and Katan MB: Bioavailability of the dietary antioxidant flavonol quercetin in man. Cancer Lett. 114:139–140. 1997. View Article : Google Scholar : PubMed/NCBI

78 

Hollman PC, van Trijp JM, Buysman MN, van der Gaag MS, Mengelers MJ, de Vries JH and Katan MB: Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Lett. 418:152–156. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Forbes AM, Lin H, Meadows GG and Meier GP: Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements. Int J Oncol 45: 831-842, 2014.
APA
Forbes, A.M., Lin, H., Meadows, G.G., & Meier, G.P. (2014). Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements. International Journal of Oncology, 45, 831-842. https://doi.org/10.3892/ijo.2014.2452
MLA
Forbes, A. M., Lin, H., Meadows, G. G., Meier, G. P."Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements". International Journal of Oncology 45.2 (2014): 831-842.
Chicago
Forbes, A. M., Lin, H., Meadows, G. G., Meier, G. P."Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements". International Journal of Oncology 45, no. 2 (2014): 831-842. https://doi.org/10.3892/ijo.2014.2452
Copy and paste a formatted citation
x
Spandidos Publications style
Forbes AM, Lin H, Meadows GG and Meier GP: Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements. Int J Oncol 45: 831-842, 2014.
APA
Forbes, A.M., Lin, H., Meadows, G.G., & Meier, G.P. (2014). Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements. International Journal of Oncology, 45, 831-842. https://doi.org/10.3892/ijo.2014.2452
MLA
Forbes, A. M., Lin, H., Meadows, G. G., Meier, G. P."Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements". International Journal of Oncology 45.2 (2014): 831-842.
Chicago
Forbes, A. M., Lin, H., Meadows, G. G., Meier, G. P."Synthesis and anticancer activity of new flavonoid analogs and inconsistencies in assays related to proliferation and viability measurements". International Journal of Oncology 45, no. 2 (2014): 831-842. https://doi.org/10.3892/ijo.2014.2452
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