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Article

Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis

  • Authors:
    • Jamie L. McClellan
    • Jennifer L. Steiner
    • Stani D. Day
    • Reilly T. Enos
    • Mark J. Davis
    • Udai P. Singh
    • E. Angela Murphy
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, Columbia, SC 29209, USA, Department of Exercise Science, School of Public Health, University of South Carolina, Columbia, SC 29208, USA
  • Pages: 861-868
    |
    Published online on: May 22, 2014
       https://doi.org/10.3892/ijo.2014.2457
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Abstract

Many observational epidemiologic studies suggest an association between exercise and colon cancer risk. The mechanisms contributing to a preventative effect of exercise on colon cancer are complex and multifaceted. Altered immune system function is one possible mechanism that has been largely unexplored. Therefore, the purpose of this study was to examine the effects of exercise on markers associated with macrophages and select T cell populations in a mouse model of intestinal tumorigenesis and to relate this to polyp characteristics. Male ApcMin/+ mice were randomly assigned to either sedentary (Sed) or exercise (Ex) treatment (n=6-9/group). The exercise treatment consisted of treadmill running for 1 h/day and 6 days a week at 15 m/min from 4 until 16 weeks of age. Intestinal polyps were counted and categorized by size. Mucosal tissue was analyzed for mRNA expression of overall macrophages (F4/80), for genes associated with M1 (IL-12, IL-23 and Nos2) and M2 (CD206, IL-10, IL-4, CCL17, CCL22 and Arg-1) macrophages and the macrophage chemoattractants MCP-1, fetuin A and CXCL14. Markers for cytotoxic T cells (CTLs) and regulatory T cells were also examined by measuring mRNA expression of CD8 and Foxp3, respectively. While there was no significant difference in overall polyp number between the groups (Sed, 23.3±4.3; and Ex, 16.5±4.3), Ex did have a reduction in the number of large polyps (Sed, 6.1±1.1; and Ex, 3.0±0.6) (P<0.05). This was consistent with a decrease in spleen weight (P<0.05). Similarly, Ex reduced mRNA expression of overall macrophages (F4/80) as well as markers associated with both M1 (IL-12) and M2 (CD206, CCL22 and Arg-1) subtypes (P<0.05) but there was no significant decrease in macrophage chemoattractants. CD8 expression was increased while Foxp3 expression was decreased with Ex (P<0.05). Overall the data provide important new information on immune regulation as a possible mechanism for the documented benefits of exercise training on reducing colon cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
McClellan JL, Steiner JL, Day SD, Enos RT, Davis MJ, Singh UP and Murphy EA: Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis. Int J Oncol 45: 861-868, 2014.
APA
McClellan, J.L., Steiner, J.L., Day, S.D., Enos, R.T., Davis, M.J., Singh, U.P., & Murphy, E.A. (2014). Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis. International Journal of Oncology, 45, 861-868. https://doi.org/10.3892/ijo.2014.2457
MLA
McClellan, J. L., Steiner, J. L., Day, S. D., Enos, R. T., Davis, M. J., Singh, U. P., Murphy, E. A."Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis". International Journal of Oncology 45.2 (2014): 861-868.
Chicago
McClellan, J. L., Steiner, J. L., Day, S. D., Enos, R. T., Davis, M. J., Singh, U. P., Murphy, E. A."Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis". International Journal of Oncology 45, no. 2 (2014): 861-868. https://doi.org/10.3892/ijo.2014.2457
Copy and paste a formatted citation
x
Spandidos Publications style
McClellan JL, Steiner JL, Day SD, Enos RT, Davis MJ, Singh UP and Murphy EA: Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis. Int J Oncol 45: 861-868, 2014.
APA
McClellan, J.L., Steiner, J.L., Day, S.D., Enos, R.T., Davis, M.J., Singh, U.P., & Murphy, E.A. (2014). Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis. International Journal of Oncology, 45, 861-868. https://doi.org/10.3892/ijo.2014.2457
MLA
McClellan, J. L., Steiner, J. L., Day, S. D., Enos, R. T., Davis, M. J., Singh, U. P., Murphy, E. A."Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis". International Journal of Oncology 45.2 (2014): 861-868.
Chicago
McClellan, J. L., Steiner, J. L., Day, S. D., Enos, R. T., Davis, M. J., Singh, U. P., Murphy, E. A."Exercise effects on polyp burden and immune markers in the ApcMin/+ mouse model of intestinal tumorigenesis". International Journal of Oncology 45, no. 2 (2014): 861-868. https://doi.org/10.3892/ijo.2014.2457
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