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Article

Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway

  • Authors:
    • Jian Zhang
    • Ju Wang
    • Jiu-Yang Jiang
    • Shang-Dian Liu
    • Kai Fu
    • Hong-Yu Liu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, P.R. China, Department of Cardiovascular Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, P.R. China
  • Pages: 683-690
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    Published online on: May 28, 2014
       https://doi.org/10.3892/ijo.2014.2471
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Abstract

Tanshinone IIA (TSIIA), a natural diterpene quinone in the traditional Chinese medicinal herb Dan-Shen (Salvia miltiorrhiza), has extensively exerted antitumor activity in cellular and animal models. However, the molecular mechanisms underlying the antitumor effects of TSIIA remain largely unknown. The in vitro effects of TSIIA on apoptosis were investigated in A549 non-small cell lung cancer (NSCLC) cells. The data showed that TSIIA significantly suppressed the proliferation of A549 cells in a dose-dependent manner, with IC50 values of 16.0±3.7 and 14.5±3.3 µM at 48 h as determined by Cell Counting Kit-8 (CCK-8) assay and clone formation assay, respectively. The change of mitochondrial morphology and the loss of mitochondrial membrane potential (MMP) were observed during the induction. Furthermore, TSIIA induced A549 cell apoptosis as confirmed by typical morphological changes, with cytochrome c release from the mitochondria and Bax translocation to the mitochondria. Caspase activity data indicated that TSIIA activated caspase-9 and caspase-3 of mitochondria-mediated apoptosis, but not caspase-8 of receptor-mediated apoptosis, which could be largely rescued by SP600125 (JNK inhibitor). Taken together, these findings provide the first evidence that TSIIA inhibits growth of NSCLC A549 cells, induces activation of JNK signaling and triggers caspase cascade apoptosis mediated by the release of cytochrome c, which provides a better understanding of the molecular mechanisms of TSIIA on lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Wang J, Jiang J, Liu S, Fu K and Liu H: Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway. Int J Oncol 45: 683-690, 2014.
APA
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., & Liu, H. (2014). Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway. International Journal of Oncology, 45, 683-690. https://doi.org/10.3892/ijo.2014.2471
MLA
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., Liu, H."Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway". International Journal of Oncology 45.2 (2014): 683-690.
Chicago
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., Liu, H."Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway". International Journal of Oncology 45, no. 2 (2014): 683-690. https://doi.org/10.3892/ijo.2014.2471
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Wang J, Jiang J, Liu S, Fu K and Liu H: Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway. Int J Oncol 45: 683-690, 2014.
APA
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., & Liu, H. (2014). Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway. International Journal of Oncology, 45, 683-690. https://doi.org/10.3892/ijo.2014.2471
MLA
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., Liu, H."Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway". International Journal of Oncology 45.2 (2014): 683-690.
Chicago
Zhang, J., Wang, J., Jiang, J., Liu, S., Fu, K., Liu, H."Tanshinone IIA induces cytochrome c-mediated caspase cascade apoptosis in A549 human lung cancer cells via the JNK pathway". International Journal of Oncology 45, no. 2 (2014): 683-690. https://doi.org/10.3892/ijo.2014.2471
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