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International Journal of Oncology
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Article

Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells

  • Authors:
    • Anna Otte
    • Finn Rauprich
    • Juliane von der Ohe
    • Peter Hillemanns
    • Ralf Hass
  • View Affiliations / Copyright

    Affiliations: Biochemistry and Tumor Biology Laboratory, Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany
  • Pages: 1151-1158
    |
    Published online on: June 24, 2014
       https://doi.org/10.3892/ijo.2014.2518
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Abstract

A recently established cellular model for the rare small cell carcinoma of the ovary hypercalcemic type (SCCOHT-1) was characterized in comparison to ovarian adenocarcinoma cells (NIH:OVCAR-3 and SK-OV-3). The different cancer populations exhibited a common sensitivity in acidic pH milieu and a continuous proliferation in alkaline medium of pH 8.0-9.0. In the presence of elevated Ca2+ concentrations, the ovarian cancer cells demonstrated a progressively reduced proliferation within 72 h in contrast to other tumor types such as breast cancer cells. This significant growth inhibition was calcium-specific since the proliferation was unaffected after culture of the ovarian cancer cells in the presence of similar concentrations of other cations. The Ca2+ effects on the ovarian cancer cells were associated with marked differences in the activation of intracellular signaling pathways including enhanced phosphorylation of the p42/44 MAP kinase (Thr202/Tyr204). Further analysis of the signaling pathway revealed a significantly enhanced Ca2+-dependent and p42/44 MAP kinase activation-mediated prostaglandin E2 (PGE2) production in SK-OV-3 and SCCOHT-1 and to a lesser extent in NIH:OVCAR-3 cells. Vice versa, exogenous PGE2 did not affect the proliferative capacity of the ovarian cancer cells and inhibition of the Ca2+-mediated MAP kinase activation did not abolish the Ca2+-mediated cytotoxicity. Collectively, these data suggest that multiple pathways are activated by exogenous Ca2+ in the different ovarian cancer cells, including a specific MAP kinase signaling cascade with subsequent PGE2 production and a parallel pathway for the induction of cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Otte A, Rauprich F, von der Ohe J, Hillemanns P and Hass R: Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells. Int J Oncol 45: 1151-1158, 2014.
APA
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., & Hass, R. (2014). Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells. International Journal of Oncology, 45, 1151-1158. https://doi.org/10.3892/ijo.2014.2518
MLA
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., Hass, R."Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells". International Journal of Oncology 45.3 (2014): 1151-1158.
Chicago
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., Hass, R."Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells". International Journal of Oncology 45, no. 3 (2014): 1151-1158. https://doi.org/10.3892/ijo.2014.2518
Copy and paste a formatted citation
x
Spandidos Publications style
Otte A, Rauprich F, von der Ohe J, Hillemanns P and Hass R: Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells. Int J Oncol 45: 1151-1158, 2014.
APA
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., & Hass, R. (2014). Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells. International Journal of Oncology, 45, 1151-1158. https://doi.org/10.3892/ijo.2014.2518
MLA
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., Hass, R."Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells". International Journal of Oncology 45.3 (2014): 1151-1158.
Chicago
Otte, A., Rauprich, F., von der Ohe, J., Hillemanns, P., Hass, R."Interference of Ca2+ with the proliferation of SCCOHT-1 and ovarian adenocarcinoma cells". International Journal of Oncology 45, no. 3 (2014): 1151-1158. https://doi.org/10.3892/ijo.2014.2518
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