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Article

Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage

Retraction in: /10.3892/ijo.2025.5759
  • Authors:
    • Zhaorui Sun
    • Hongmei Liu
    • Zhizhou Yang
    • Danbing Shao
    • Wei Zhang
    • Yi Ren
    • Baodi Sun
    • Jinfeng Lin
    • Min Xu
    • Shinan Nie
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China
  • Pages: 1123-1132
    |
    Published online on: June 27, 2014
       https://doi.org/10.3892/ijo.2014.2527
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Abstract

Intestinal trefoil factor (ITF, also named as trefoil factor 3, TFF3) is a member of the TFF-domain peptide family, which plays an essential role in the regulation of cell survival, cell migration and maintains mucosal epithelial integrity in the gastrointestinal tract. However, the underlying mechanisms and associated molecules remain unclear. The aim of this study was to explore the protective effects of ITF on gastric mucosal epithelium injury and its possible molecular mechanisms of action. In the present study, we show that ITF was able to promote the proliferation and migration of GES-1 cells via a mechanism that involves the PI3K/Akt signaling pathway. Western blot results indicated that ITF induced a dose- and time-dependent increase in the Akt signaling pathway. ITF also plays an essential role in the restitution of GES-1 cell damage induced by lipopolysaccharide (LPS). LPS induced the apoptosis of GES-1 cells, decreased cell viability significantly (P<0.01) and led to epithelial tight junction damage, which is attenuated via ITF treatment. The protective effect of ITF on the integrity of GES-1 was abrogated by inhibition of the PI3K/Akt pathway. Taken together, our results demonstrate that ITF promotes the proliferation and migration of gastric mucosal epithelial cells and preserves gastric mucosal epithelial integrity after damage is mediated by activation of the PI3K/Akt signaling pathway. This study suggested that the PI3K/Akt pathway could act as a key intracellular pathway in the gastric mucosal epithelium that may serve as a therapeutic target to preserve epithelial integrity during injury.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Liu H, Yang Z, Shao D, Zhang W, Ren Y, Sun B, Lin J, Xu M, Nie S, Nie S, et al: Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759. Int J Oncol 45: 1123-1132, 2014.
APA
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y. ... Nie, S. (2014). Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759. International Journal of Oncology, 45, 1123-1132. https://doi.org/10.3892/ijo.2014.2527
MLA
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y., Sun, B., Lin, J., Xu, M., Nie, S."Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759". International Journal of Oncology 45.3 (2014): 1123-1132.
Chicago
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y., Sun, B., Lin, J., Xu, M., Nie, S."Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759". International Journal of Oncology 45, no. 3 (2014): 1123-1132. https://doi.org/10.3892/ijo.2014.2527
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Z, Liu H, Yang Z, Shao D, Zhang W, Ren Y, Sun B, Lin J, Xu M, Nie S, Nie S, et al: Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759. Int J Oncol 45: 1123-1132, 2014.
APA
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y. ... Nie, S. (2014). Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759. International Journal of Oncology, 45, 1123-1132. https://doi.org/10.3892/ijo.2014.2527
MLA
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y., Sun, B., Lin, J., Xu, M., Nie, S."Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759". International Journal of Oncology 45.3 (2014): 1123-1132.
Chicago
Sun, Z., Liu, H., Yang, Z., Shao, D., Zhang, W., Ren, Y., Sun, B., Lin, J., Xu, M., Nie, S."Intestinal trefoil factor activates the PI3K/Akt signaling pathway to protect gastric mucosal epithelium from damage Retraction in /10.3892/ijo.2025.5759". International Journal of Oncology 45, no. 3 (2014): 1123-1132. https://doi.org/10.3892/ijo.2014.2527
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