Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action

  • Authors:
    • Yun Fu
    • Yingli Yang
    • Sufeng Zhou
    • Youxun Liu
    • Yanbin Yuan
    • Shaoshan Li
    • Changzheng Li
  • View Affiliations

  • Published online on: August 19, 2014     https://doi.org/10.3892/ijo.2014.2611
  • Pages: 2092-2100
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Abstract

The Mannich base containing ciprofloxacin and kojic acid structural units was prepared and evaluated in antitumor activity. The enhancement in antitumor activity was observed both from the Mannich base (IC50: 103.3±5.0 µM for HepG2, 87.9±8.0 µM for HCT-116 cell) and its copper complex (IC50: 11.5±1.8 µM for HepG2, 44.4±2.5 µM for HCT-116 cell) compared to the ciprofloxacin and kojic acid. The mechanistic studies via RT-PCR, cell cycle analysis, mitochondrial membrane potential measurement, inhibition of topoisomerase and molecular docking indicated that there is a different molecular mechanism between the Mannich base and its copper complex. The cytotoxicity of the Mannich base was involved in apoptosis, cell cycle arrest, depolarization of mitochondrial membrane and weaker topoisomerase II inhibition, but the copper complex exerted its cytotoxicity mainly through dual topoisomerase inhibition, especially stabilizing the intermediate of cleavage DNA-topoisomerase complex.

Introduction

Kojic acid (5-hydroxy-2-hydroxymethyl-4H-pyran-4-one) is a biologically important natural product and used widely as an additive in the food industry and bleaching agent in cosmetic preparations (1). Kojic acid and its derivatives also possess other various bioactivities such as antimicrobial (2,3), antidiabetic (4) and antitumor activity (5). Moreover, it can chelate many metal ions as bidentate ligand, such copper (6) and its skin lightening ability is stemmed from its copper-chelating property. Copper chelation is currently being investigated as an antiangiogenic and antineoplastic agent in patients diagnosed with esophageal carcinoma, hormone-refractory prostate cancer, colorectal cancer, and breast cancer (7). In light of its copper-chelating property with tyrosinase, kojic acid appears to be a potential candidate to develop novel classes of metal-chelating antiproliferative agents (8), and many of its derivatives of it have been synthesized to improve their biological activity (9,10).

The fluoroquinolones belong to the group of synthetic antibiotics that exert broad-spectrum antimicrobial activities. Ciprofloxacin is one of the fluoroquinolones and widely used in clinic as antimicrobial agent. In addition to antimicrobial activity, antitumor activity against colon cancer, bladder cancer, leukemia and hepatic cell lines have been found and linked to topoisomerase II (Top II) inhibitory (1113). The structure-activity relationship (SAR) and the functions of different positions on the quinolone core have been identified (14). The SAR studies indicated that the group at position 7 affects its antibacterial spectrum (15). The substituted 7-piperazinyl derivatives of ciprofloxacin, such as azo (11), Mannich base (16,17), N-alkylated (18) and N-acylated ciprofloxacin derivatives (19) have been synthesized and evaluated for antibacterial activity.

Combined multifunctional units into a molecule is a strategy of fragment-based drug discovery to find lead compounds for different research aims, the Mannich reaction is one of ways used to perform this task. In addition, the Mannich bases themselves have good biological activity, so many of these compounds were synthesized and investigated for antimicrobial and antitumor activities. In our previous investigation (13), we found that the structural modification on ciprofloxacin may improve its antitumor activity, as extended study, the compound of 7-piperazinyl ciprofloxacin with kojic acid and its copper complex were synthesized in order to investigate whether there is potent enhancement in antitumor activities. In this study, the potent molecular mechanism of action of the Mannich base and its copper complex was explored via RT-PCR, cell cycle analysis, topoisomerase inhibition and molecular docking, revealing a new action mechanism for the copper complex.

Materials and methods

Materials

All chemical reagents were analytical reagent grade. MTT, rhodamine 123, propidium iodide, ethidium bromide and SDS were purchased from Sigma-Aldrich, Inc. (Shanghai, China). Ciprofloxacin hydrochloride was obtained from Sangon Biotech (Shanghai, China). Kojic acid was purchased from Aladdin Industrial Corp. (Shanghai, China).

Preparation of Mannich base of kojic acid with ciprofloxacin

Mannich bases were prepared by the reaction of ciprofloxacin (0.01 mol) and kojic acid (0.01 mol) in methanol with 37% formalin (0.012 mol). The mixture was stirred vigorously for 4 h. The resulting precipitate was collected by filtration and washed with cold methanol. The crude product was recrystallized from methanol. The Mannich base was characterized by 1H NMR (400 MHz, Bruker, Switzerland) spectrum and Mass spectrum: 1H NMR (d6-DMSO) 1H NMR d (DMSO-d6, 400 MHz) δ (ppm): 2.61 (4H; t; piperazine), 3.35 (4H; s; piperazine), 3.58 (2H; s; -CH2-), 4.34 (2H; s; -HOCH2-), 5.77 (1H; t; CH2-OH), 6.38 (1H; s; H5-pyran), 9.45 ppm (1H; s; 3-OH pyran), 1.18 – 1.32 (m, 4H, -CH2CH2-), 3.16 – 3.24 (m, 8H, piperazine-H), 3.35 (m, 1H, CH), 7.58 (d, 1H, J = 7.2 Hz, H8 of cip), 7.89 (d, J = 13.5 Hz, 1H, H5 of cip), 8.66 (s, 1H, H2 of cip), 15.16 (s, 1H, -COO H); MS (VG Autospec-3000 spectrometer, Micromass, UK): m/e 485.46. The structure of the Mannich base and its copper complex are shown in Fig. 1.

Cell culture and cytotoxicity assay

HCT-116 and HepG2 human hepatoma cell lines (Y-S Biotechnology Inc., Shanghai, China) were propagated continuously in RPMI-1640 medium supplemented with 10% freshly inactivated fetal calf serum and antibiotics (100 U/ml penicillin G, and 100 μg/ml streptomycin) in a humidified atmosphere of 5% CO2 and 95% air at 37°C. The HCT-116 and HepG2 cells in exponential-phase were detached by 0.25% trypsin, followed by centrifugation, PBS washing, and re-suspended in RPMI-1640, then the equal cells were seeded (5×103/well) into a 96-well plate. When the cells attached to the Mannich base the copper complex was added at the final concentrations at 200, 100, 50, 25, 12.5, 6, 3 and 1.5 μM which varied with the drug and cell line. After incubation for 48 h, 10 μl MTT solution (1 mg/ml) was added to each well, the plate was further incubated for 4 h. The cell culture was removed by aspiration and 100 μl DMSO was added into each well to dissolve the formazan crystals. The measurement of absorbance at 570 nm was performed on an ELISA spectrophotometer (MK3, Thermo Scientific, Shanghai, China). Percent growth inhibition was defined as percent absorbance inhibition within appropriate absorbance in each cell line. The same assay was done in triplet.

RT-PCR

Total RNA was extracted from the HepG2 cells using TRIzol reagent (Shangong Biological, Shanghai, China) according to the manufacturer’s protocol. Three micrograms of total RNA were used for reverse transcription in a total volume of 20 μl following the manufacturer’s recommendation (Lifefeng, Shanghai, China). Aliquots of 2 μl cDNA were subsequently amplified in a total volume of 25 μl. The sense and antisense primer for β-actin were 5′-ACACTGTGCC CATCTACGAGG-3′ and 5′-CGGACTCGTCATACTCC TGCT-3′ (615 bp) that were used as an internal control; the sense and antisense primers for bcl2 were 5′-TTACCAAGCAG CCGAAGA-3′ and 5′-TCCCTCCTTTACATTCACAA-3′ (309 bp, NM_ 138621); the sense and antisense primers for bax, 5′-TTTTGCTTCAGGGTTTCATC-3′ and 5′-GGCCTT GAGCACCAGTTT-3′ (299 bp, BC014175); the sense and antisense primers for p53, 5′-GTCTACCTCCCGCCATAA-3′, 5′-CATCTCCCAAACATCCCT-3′ (316 bp, NM_ 001126114.2); the sense and antisense primers for cyclin A, 5′-TTAGGGAAA TGGAGGTTA-3′ and 5′-CAGAAAGTATTGGGTAAGAA-3′ (404 bp, NM_001237.3); the sense and antisense primers for cyclin B, 5′-TCTGCTGGGTGTAGGTCC-3′ and 5′-AATA GGCTCAGGCGAAAG-3′ (444 bp, NM_0319 66.3); the sense and antisense primers for cyclin D1, 5′-CTGGATGCTGGAGG TCTGCGAGGA-3′ and 5′-TGAACTTCACATCTGTGGCAC AGA-3′ (400 bp, M 73554), respectively. The cycling conditions: 94°C for 5 min, followed by 28 cycles of 94°C for 30 sec, 58°C for 30 sec and 72°C for 1 min, and a final extension of 72°C for 10 min. PCR products were separated on the 1.5% agarose gel viewed by ethidium bromide staining. These data were acquired with Tocan 360 gel imager (version 3.2.1 software, Shanghai Tiancheng Technology Inc., Shanghai, China). The RT-PCR experiments were done in duplicates.

Cell cycle analysis

HepG2 cells (1×105) were seeded in a 6-well plate. After 24-h incubation at 37°C (5% CO2), the medium was changed with fresh, supplemented or not (control) with the Mannich base (50 and 100 μM). After 24-h incubation, cells were harvested with trypsin, washed by PBS, fixed in 70% ethanol and stored at −20°C for 1 h. The cellular nuclear DNA was stained by propidium iodide (PI) as described (13), briefly, followed by removing the ethanol, washed with PBS, the cells were suspended in 0.5 ml PBS containing 50 μg/ml PI and 100 μg/ml RNase and incubated at 37°C for 30 min. Flow cytometry was performed in duplicate with a FACScalibur flow cytometer (Becton-Dickinson, USA). From each sample 10,000 events were collected and fluorescent signal intensity was recorded and analyzed by CellQuest and Modifit (Becton-Dickinson).

Flow cytometry analysis of mitochondrial membrane potential

Following similar procedure as mentioned in cell cycle analysis, the HepG2 cells were treated with the Mannich base (50 and 100 μM) or its copper complex (6 and 12 μM) for 24 h, the cells were washed with PBS twice after removing the cell medium and digested with trypsin. Treated cells were collected and resuspended at a concentration of 1×105/ml in PBS containing 1 μmol/l rhodamine 123 and then incubated at 37°C for 30 min for direct use in flow cytometry. Samples were analyzed by FACSCalibur flow cytometer (Becton-Dickinson) with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. Data collection and analysis were as described above.

DNA Top activity assay

The nuclear extract from HepG2 cells was prepared as described (13). Briefly, cells were detached using trypsin followed by centrifugation and washing twice with cold nucleus buffer (NB) (150 mM sodium chloride, 1 mM potassium dihydrogen phosphate, 5 mM magnesium chloride, 1 mM EDTA, 2 mM dithiotreitol and 1 mM PMSF, pH 6.4) at 4°C. The supernatant was discarded and the cell pellet was re-suspended in 300 μl NB with 0.3% Triton X-100 and placed on ice for 10 min, then transferred into a glass Dounce homogenizer with ten up-and-down strokes using a loose-fitting pestle. The suspension solution was centrifuged at 150 × g for 10 min at 4°C, the pellet washed with Triton X-l00-free cold NB. Cold NB solution (150 μl) containing 0.35 M NaCl was added to the pellet and allowed to stand 30 min at 4°C in order to extract the nuclear proteins. The supernatant was obtained by centrifugation at 10,000 × g for 10 min at 4°C. The protein concentration was determined using the Bradford method. Nuclear extract (0.4 μg) was added to the Top reaction mixture containing 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 150 mM NaCl, 0.1% BSA (bovine serum albumin), 0.1 mM spermidine, 5% glycerol and 0.4 μg pUC18 and 3 μl (or 2, or 1 μl) test compound (1 or 0.3 mM) at a final volume of 10 μl. Following incubation at 37°C for 30 min, the reaction was terminated by adding 5 μl of stopping buffer (10% SDS, 0.025% bromophenol blue and 5% glycerol). The reaction products were analyzed by electrophoresis on 1% agarose gel using a TBE buffer with 0.1% SDS (89 mM Tris-HCl, 89 mM boric acid and 62 mM EDTA) at 45 V for 3 h, stained by ethidium bromide (0.5 μg/ml) and photographed using a short wavelength UV lamp on Tocan 360 gel scanner (Shanghai Tiancheng Technology Inc.). The assay was conducted in duplicate.

Molecular docking studies

The structure of human type I DNA Top with DNA and Topotecan (1K4T) and human type II Top (3QX3) were obtained from RCSB Protein Data Bank (20). The Mannich base was generated from Chemdraw (Chemdraw Ultra 8.0, CambridgeSoft, USA). Similarly structure of its copper complex was proposed based on copper (II) ion coordination geometry in general. The energy minimization was conducted by Chem3D (Ultra 8.0, CambridgeSoft) (21). The resulting models were displayed in PyMol (The PyMOL Molecular Graphics System, Version 1.4.1, Schrödinger, LLC, USA).

Molecular docking studies were performed by AutoDock Vina and AutoDock Tools based on the recommended procedure (22). Grid boxes were set to the center of ciprofloxacin (or topotecan) model, and the grid box size for Mannich base models was set to 22, 24, and 28 for x-, y- and z-axis, respectively. The Mannich base was set as a flexible ligand by using the default parameters of the AutoDock Tools. The optimal conformation of the ligand was generated by AutoDock Vina.

Results

Antitumor activity

It has been demonstrated that ciprofloxacin and kojic acid exhibit their cytotoxicity against some tumor cell lines. The Mannich base containing ciprofloxacin structural unit might have similar property. To determine their potential antitumor activity, the proliferation inhibition of the Mannich base against HepG2 and HCT-116 cell lines was investigated by MTT assay (Fig. 2). For both cell lines, the Mannich base was shown to be more toxic than ciprofloxacin, with IC50 = 103.3±5.1 μM for HepG2, and 87.9±8.0 μM for HCT-116, respectively (Fig. 2). Due to copper chelator ability of kojic acid, the copper complex of the Mannich base was prepared and evaluated. It is notable that the copper complex exhibited excellent biological activities and selectivity, indicating there is a synergistic effect when the copper ion was chelated by the Mannich base. The IC50 of the copper complex was 11.5±3.0 μM for HepG2 cell, a ~10-fold decrease in IC50 compared to that of the Mannich base. Similar trend against HCT-116 cell line was also observed, except the 2-fold dropped IC50 (87 vs 44 μM in IC50). The copper complex seemed to exhibit to some extent selectivity for HepG2 cell.

The regulation of the Mannich base and its copper complex on apoptotic gene expression

The Mannich base and its copper complex showed good antitumor activities, it was necessary to determine their action mechanism. Ciprofloxacin induces apoptosis via regulation of pro-apoptotic Bax, anti-apoptotic Bcl-2 genes to exert its cytotoxicity, so RT-PCR was employed to evaluate the gene regulation of the Mannich base and its copper complex. As shown in Fig. 3a, Bax increased dose-dependently after 24 h of incubation of the Mannich base, while Bcl-2 was decreased. For easy comparison, the ratio of Bax/Bcl-2 was set at 1.0 in untreated cells, and the changes are shown in Fig. 3c (narrow bar). It was clear that the Bax/Bcl-2 ratio in the Mannich base treated cells was significantly increased in a concentration-dependent manner, indicating that the cytotoxicity of the investigated compounds might be involved in apoptosis. The cell cycle factor was also checked, it is of interest that the cyclin D1 was downregulated, which was correlated to G1 arrest. However, other cell cycle factors and p53 were not clearly changed (Fig. 3a). Similar procedure was applied on the gene regulation of the copper complex (Fig. 3b), the investigated gene expression was not significantly changed except the Bax slight increased, showing that a different way occurred compared to the Mannich base.

Cell cycle arrest

The proliferation inhibition may stem from disturbing the cell cycle, to test the hypothesis, cell cycle analyses were conducted after 24-h exposure of the drugs to HepG2 cells under IC50. As shown in Fig. 4a–c, the Mannich base caused an accumulation of cells in the G1-phase. The percentage of cells at the G1-phase significantly increased from 58.43 to 76.14 and 70.67% after treatment with 50 and 100 μM Mannich base, respectively. The result indicated that the anti-proliferative effect of the Mannich base could be partly derived from inducing cell cycle arrest. When the cells were treated with the copper complex, the G1 arrest was not observed (Fig. 4d–f), indicating that the cytotoxicity of the copper complex was not due to the disturbance of cell cycle.

Mitochondrial membrane potential

Ciprofloxacin can depolarize the mitochondrial membrane and inhibit mtDNA synthesis, so it was necessary to determine whether the Mannich base and its copper complex has similar function. The depolarization of mitochondrial membrane was evaluated via rhodamine 123 staining on BD flow cytometry. The results are shown in Fig. 5. Compared to control, the distribution of cells with different fluorescence was significantly altered both for the Mannich base and its copper complex treated groups. Almost all the cells (87–95%) were in M2 gate after the drugs treatments, a ~38–46% increase could be seen (Fig. 5). These observations indicated that both the Mannich base and its copper complex could depolarize the mitochondrial membrane, decreasing its electrochemical potential.

Inhibition of DNA human Top

Ciprofloxacin as a type II DNA Top inhibitor is well documented. To determine whether the Mannich base and its copper complex recapitulate such activity, plasmid DNA was incubated with nucleic extract in the presence of a varied concentration of the investigated compounds, and reaction products were analyzed by gel electrophoresis. As shown in Fig. 6a, the Mannich base exhibited inhibition of human Top II (at 300 μM), but the Top I inhibition was not observed. The copper complex exhibited dual Top inhibition both for type I and II at much lower concentration (Fig. 6b). To further reveal the action mode of the copper complex in Top inhibition, the reaction mixtures were subjected to electrophoresis on EtB containing agarose gel. As shown in the gel (Fig. 6c), the topology was dominated by the intercalative dye, all closed circular forms of DNA would be positively supercoiled and migrate with similar rate. The Top II cleavage complex could be identified by comparison with the positive control of etoposide. From Fig. 6c, the Top II-DNA cleavage complex was seen and the amount of cleavage DNA was increased with increasing of the concentration of the copper complex, indicating that the potent cytotoxicity of the copper complex may contribute to stabilize the Top II-DNA cleavage complex.

Molecular docking analysis

Top inhibition exerted by the copper complex in vitro made us look for potent structural basis, the molecular docking was conducted. The human type I and II Top crystal structures were from RCSB Protein Data Bank. In order to evaluate the accuracy of our docking protocol, etoposide and topotecan were re-docked into each Top-DNA complex based on their crystal structures. The human type I (PDB ID:1K4T) and II Top (PDB ID:3QX3) were chosen as receptors, and the copper complex was used as ligand. The input pbdqt files were generated by Autodocktool and the optimal conformations of ligands were calculated on AutoDock Vina. The Top-inhibitor complex derived from docking simulation showed that the docked etoposide and topotecan were almost fully superimposed on the native co-crystallized ones (Fig. 7a and d). Following the same protocol the copper complex was individually docked into the Top I and II, the simulating affinity energy was −11.5 and −10.9 kcal/mol, respectively. Compared to that of redocked etoposide, −14.6 and topotecan, −11.2 kcal/mol, the affinity energy of the copper complex with the Top was quite close to the clinically used drugs. The orientation of the copper complex in the Top I and II is shown in Fig. 7b and e, respectively. In addition, the superimposition of the copper complex on the co-crystallized etoposide and topotecan are presented in Fig. 7c and f.

Discussion

Ciprofloxacin has a certain degree of antitumor activity (23), so does the ciprofloxacin containing Mannich base. The proliferation inhibition of the Mannich base clearly showed that the structural modification on 7-piperdinyl ciprofloxacin with kojic acid unit leads to enhancement in antitumor activity. However, its antimicrobial activities was decreased from that reported recently (16). It should be realized that ciprofloxacin and kojic acid both have potential chelating ability, and the derivatives of kojic acid have been synthesized as a copper chelator, corresponding metal complexes of the derivatives have also been investigated (24,25). The excellent proliferation inhibition of the copper complex encouraged us to probe the potent molecular mechanism. A previous study showed that the copper chloride has no detectable cytotoxicity at 50 μM (data not shown), so the enhancement of the copper complex of the Mannich base in proliferation inhibition could not be simply explained as synergistic effect because synchronous exposure of the copper chloride and the Mannich base to the investigated cell lines did not induce higher cytotoxicity (data not shown). However, after adjusting the pH of Mannich base with base, a copper chloride addition leads to a suspension with color change, indicating the copper complex of the Mannich base was formed (26). Thus additional studies are required to probe potent molecular mechanism, which may provide some meaningful information to explain the phenomenon. It has been well demonstrated that many chemotherapeutic agents exert their cytotoxicity via apoptotic path. Bax and Bcl-2 are apoptosis-related genes, the Bax upregulation and Bcl-2 downregulation are indicative of apoptosis involved. In our study the RT-PCR data clearly show that the Bax/Bcl-2 ratio in the Mannich base treated HepG2 cells was significantly increased, indicating that the Mannich base induced apoptosis via the same molecular path as ciprofloxacin. However, this situation for its copper complex was not observed, indicating the cytotoxicity of the copper complex might not be involved in apoptotic pathway.

It has been demonstrated that quinolone derivatives induce cell death through causing double-stranded DNA breakage, inhibition of Top function (27,28), so the ciprofloxacin containing Mannich base in this study might share a similar action mechanism. To test the hypothesis Top inhibition assay was carried out. Unsurprisingly, type II topoisomerase inhibition by the Mannich base was observed at higher concentration, but the type I Top inhibition was not evident. Beyond our expectations, the copper complex exhibited dual Top inhibition, this situation has been only found in a few metal complexes (2932), so we speculated that the excellent antitumor activity may be closely related to its dual Top inhibition. However, due to lack of evidence from crystal structure, how this kind of metal complex interacts with Top, via competitive ATP binding or poisoning DNA complex or allosteric effect to disturb unwinding DNA helix are largely unknown. In our experiment, the DNA-Top complex poisoning function of the copper complex could be seen, stabilizing the intermediate and causing more DNA cleavage in a concentration-dependent manner. As mentioned above due to lack of detailed structural information, in silico study to simulate the potent interaction between the copper complex and the DNA-Top complex may give some helpful information. Molecular docking is a well established computational technique to predict the interaction between enzyme and inhibitor (33,34). In addition, AutoDock software is widely used to predict the binding information of test compounds (35,36). In general, divalence copper ion (II) can be chelated by many compounds contained heteroatom, such as kojic acid, forming square planar molecules in geometry (37). So 1:2 molar ratio of copper ion to the Mannich base may be speculated, and a square planar geometry for the copper complex was tentatively proposed for the docking study. In addition, in this case, the copper complex may have non-electrolyte property and thus can easily cross the cellular membrane. It is interesting that the docked copper complex has affinity energy with the Top I or II quite close to that of etoposide or topotecan used in clinic, indicating the copper complex might have similar manner as poison to stabilize the cleavage DNA-Top complex and cause DNA fragmentation. The data from molecular docking was supportive of the Top inhibition experiment in vitro and corelated with assessment of the cytotoxicity of the complex.

Top IIα inhibition or poisoning leads G2/M-phase arrest in general (38,39), to look for further support, the cell cycle was assessed in the presence of the investigated compounds. Surprisingly, both the Mannich base and its copper complex did not lead to G2/M-phase arrest. It unambiguously demonstrated that the Mannich base caused a G1-phase arrest. Cyclin D1 plays an important role in regulating cell cycle progression and cyclin D1 degradation is sufficient to induce G1 cell cycle arrest (38). The cyclin D1 downregulation at mRNA level in the treatment of Mannich base might be also correlated with the G1 arrest. This situation was also found in Top inhibitors, for the cell cycle arrest dose-dependently in some cell lines (40,41). Unlike the Mannich base, its copper complex was not able to disturb the cell cycle, indicating that the mechanisms between them were different.

Mitochondria are producing energy plants, and the ATP was produced by utilizing the proton electrochemical gradient potential between inner and outer mitochondrial membrane. The depolarization of mitochondrial membrane will result in decreases of the potential and proton gradient, consequently leading to less efficient ATP production (42). Our observations also support that both the Mannich base and its copper complex have functions of depolarization of mitochondrial membrane compared to the untreated group from the fluorescence intensity of mitochondria stained by rhodamine 123 (Fig. 6), which cause ATP shortage, consequently slowing down cell growth. So it is reasonable to speculate that the disturbance of mitochondrial respiration and ATP synthesis may contribute to the cytotoxic effect of the Mannich or its copper complex (43).

In conclusion, the Mannich base derived from ciprofloxacin is more toxic than its parent compound in antitumor activity. Its copper complex showed excellent antitumor activity. Mechanistic studies revealed preliminarily that the Mannich base exerted cytotoxicity via regulation of a pro-apoptotic gene, causing cell cycle arrest, disturbing ATP production and inhibiting Top II. Unlike the Mannich base, the cytotoxicity of its copper complex mainly stem from its ability to stabilize the intermediate of cleavage DNA-Top complex as well as disturbance of the mitochondrial respiration chain. However, detailed mechanism of the copper complex, especially in Top inhibition in vivo is largely unknown and require more study in the future.

Acknowledgements

This study was supported by grants CP1204 from Xinxiang Scientific and Technology Division; ZD2011-06 from Xinxiang Medical University and 2109901 from plan of Health Scientific and Technological Innovation Talents of Henan Province for Shaoshan Li.

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November-2014
Volume 45 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Fu Y, Yang Y, Zhou S, Liu Y, Yuan Y, Li S and Li C: Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action. Int J Oncol 45: 2092-2100, 2014
APA
Fu, Y., Yang, Y., Zhou, S., Liu, Y., Yuan, Y., Li, S., & Li, C. (2014). Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action. International Journal of Oncology, 45, 2092-2100. https://doi.org/10.3892/ijo.2014.2611
MLA
Fu, Y., Yang, Y., Zhou, S., Liu, Y., Yuan, Y., Li, S., Li, C."Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action". International Journal of Oncology 45.5 (2014): 2092-2100.
Chicago
Fu, Y., Yang, Y., Zhou, S., Liu, Y., Yuan, Y., Li, S., Li, C."Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action". International Journal of Oncology 45, no. 5 (2014): 2092-2100. https://doi.org/10.3892/ijo.2014.2611