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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells

  • Authors:
    • Dandan Yuan
    • Hongwei Xia
    • Yuchen Zhang
    • Liang Chen
    • Weibing Leng
    • Tie Chen
    • Qingjuan Chen
    • Qiulin Tang
    • Xianming Mo
    • Ming Liu
    • Feng Bi
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China, Laboratory of Signal Transduction and Molecular Target Therapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China, Laboratory of Stem Cell Biology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China
  • Pages: 2430-2438
    |
    Published online on: September 9, 2014
       https://doi.org/10.3892/ijo.2014.2644
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Abstract

Both circulating tumor cells (CTCs) and epithelial-mesenchymal transition (EMT) play an important role in invasion, migration and chemoresistant in tumor development. This study aimed to detect whether EMT occurred in human gastric CTCs and to explore the mechanism of EMT in human gastric CTCs. We analysed epithelial markers (pan-CK, E-cadherin), mesenchymal markers (N-cadherin, vimentin) EMT related miR‑200s, and Akt in gastric CTCs. The impact of miR‑200s on EMT, migration and invasion in CTCs was tested. We found that epithelial markers pan-CK, E-cadherin were decreased, and mesenchymal markers N-cadherin, vimentin were overexpressed in gastric CTCs. Expression of EMT related transcriptors, snail1, zeb1, twist1, were reversely correlated with miR‑200s, and were positively correlated with phospho-Akt. Upregulated of miR‑200s downregulated twist1 and zeb1 mRNA expression, and resulted in the supression of EMT, and impaired migration and invasion in gastric CTCs. Inhibition of p-Akt led to upregulation of miR‑200s. In conclusion, gastric CTCs exhibited remarkable EMT process, and p-Akt/miR‑200s signaling regulates EMT, migration and invasion in gastric CTCs.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan D, Xia H, Zhang Y, Chen L, Leng W, Chen T, Chen Q, Tang Q, Mo X, Liu M, Liu M, et al: P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells. Int J Oncol 45: 2430-2438, 2014.
APA
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T. ... Bi, F. (2014). P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells. International Journal of Oncology, 45, 2430-2438. https://doi.org/10.3892/ijo.2014.2644
MLA
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T., Chen, Q., Tang, Q., Mo, X., Liu, M., Bi, F."P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells". International Journal of Oncology 45.6 (2014): 2430-2438.
Chicago
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T., Chen, Q., Tang, Q., Mo, X., Liu, M., Bi, F."P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells". International Journal of Oncology 45, no. 6 (2014): 2430-2438. https://doi.org/10.3892/ijo.2014.2644
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan D, Xia H, Zhang Y, Chen L, Leng W, Chen T, Chen Q, Tang Q, Mo X, Liu M, Liu M, et al: P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells. Int J Oncol 45: 2430-2438, 2014.
APA
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T. ... Bi, F. (2014). P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells. International Journal of Oncology, 45, 2430-2438. https://doi.org/10.3892/ijo.2014.2644
MLA
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T., Chen, Q., Tang, Q., Mo, X., Liu, M., Bi, F."P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells". International Journal of Oncology 45.6 (2014): 2430-2438.
Chicago
Yuan, D., Xia, H., Zhang, Y., Chen, L., Leng, W., Chen, T., Chen, Q., Tang, Q., Mo, X., Liu, M., Bi, F."P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells". International Journal of Oncology 45, no. 6 (2014): 2430-2438. https://doi.org/10.3892/ijo.2014.2644
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