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December-2014 Volume 45 Issue 6

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Article

DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling

  • Authors:
    • Dong Wang
    • Sheng Han
    • Rui Peng
    • Chenyu Jiao
    • Xing  Wang
    • Zeguang Han
    • Xiangcheng Li
  • View Affiliations / Copyright

    Affiliations: Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu, P.R. China, Shanghai‑MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, P.R. China
  • Pages: 2596-2604
    |
    Published online on: September 16, 2014
       https://doi.org/10.3892/ijo.2014.2653
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Abstract

DUSP28, a member of the atypical dual‑specificity phosphatase (DUSP) family, is a candidate tumor‑related gene in hepatocellular carcinoma (HCC) selected by genome‑wide approach, but its pathological role in HCC has not been elucidated. Here, we report for the first time that DUSP28 is involved in HCC progression. Quantitative real‑time PCR and semi‑quantitative RT‑PCR showed notably elevated expression of DUSP28 in HCC specimens compared to that in corresponding adjacent non‑tumor liver. DUSP28 overexpression promoted HCC cell proliferation, colony formation and soft agar colony formation in vitro while DUSP28 knockdown resulted in the opposite effects. Furthermore, the flow cytometric analysis indicated that DUSP28 could lead to an increased population of cancer cells in S phase, with a concomitant decrease of cells in G1 phase. Investigation of the mechanism revealed that DUSP28 could activate the p38 mitogen‑activated protein kinase (MAPK) signaling pathway. Taken together, these data demonstrate that DUSP28 plays a significant role in HCC progression and may be a feasible molecular target for anti‑cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Han S, Peng R, Jiao C, Wang X, Han Z and Li X: DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling. Int J Oncol 45: 2596-2604, 2014.
APA
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., & Li, X. (2014). DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling. International Journal of Oncology, 45, 2596-2604. https://doi.org/10.3892/ijo.2014.2653
MLA
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., Li, X."DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling". International Journal of Oncology 45.6 (2014): 2596-2604.
Chicago
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., Li, X."DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling". International Journal of Oncology 45, no. 6 (2014): 2596-2604. https://doi.org/10.3892/ijo.2014.2653
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Han S, Peng R, Jiao C, Wang X, Han Z and Li X: DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling. Int J Oncol 45: 2596-2604, 2014.
APA
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., & Li, X. (2014). DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling. International Journal of Oncology, 45, 2596-2604. https://doi.org/10.3892/ijo.2014.2653
MLA
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., Li, X."DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling". International Journal of Oncology 45.6 (2014): 2596-2604.
Chicago
Wang, D., Han, S., Peng, R., Jiao, C., Wang, X., Han, Z., Li, X."DUSP28 contributes to human hepatocellular carcinoma via regulation of the p38 MAPK signaling". International Journal of Oncology 45, no. 6 (2014): 2596-2604. https://doi.org/10.3892/ijo.2014.2653
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