Biphasic ER-α36-mediated estrogen signaling regulates growth of gastric cancer cells

Wang,Xuming; Huang,Xuan; Fu,Zhengqi; Zou,Feng; Li,Yan; Wang,Zhaoyi; Liu,Lijiang

doi:10.3892/ijo.2014.2660

December-2014 Volume 45 Issue 6

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December-2014 Volume 45 Issue 6

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Article

Biphasic ER-α36-mediated estrogen signaling regulates growth of gastric cancer cells

Authors:
- Xuming Wang
- Xuan Huang
- Zhengqi Fu
- Feng Zou
- Yan Li
- Zhaoyi Wang
- Lijiang Liu
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Affiliations: Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan, P.R. China, Department of Medical Microbiology and Immunology, Creighton University Medical School, Omaha, NE, USA
Pages: 2325-2330
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Published online on: September 17, 2014

https://doi.org/10.3892/ijo.2014.2660
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Abstract

To examine the expression patterns of ER-α36 and Cyclin D1 in human gastric cancer tissues and to investigate the effects of ER-α36-mediated estrogen signaling on the growth of gastric cancer cells, 117 samples of formalin-fixed and paraffin-embedded gastric cancer tumor tissues and 40 fresh gastric cancer tumor tissues were analyzed with immunohistochemistry assay and western blot analysis. ER-α36 expression was well correlated with gender (male:female ratio 2.88:1, P=0.01), invasion to serosa (P=0.01) as well as Cyclin D1 expression (P<0.01). The effects of different concentrations of estrogen on the growth of different gastric cancer cells and normal gastric cells as well as gastric cancer SGC7901 cells with different levels of ER-α36 expression were examined. SGC7901 cells with high levels of ER-α36 expression exhibited estrogen hypersensitivity, high growth rate and high levels of Cyclin D1 expression while SGC7901 cells with knocked-down levels of ER-α36 expression were insensitive to estrogen stimulation, grew slowly and expressed less Cyclin D1. Our results indicate that ER-α36 mediates biphasic estrogen signaling in the growth of gastric cancer cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Biphasic ER-α36-mediated estrogen signaling regulates growth of gastric cancer cells

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Abstract

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