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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December-2014 Volume 45 Issue 6

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments

  • Authors:
    • Alexander Glassmann
    • Jochen Winter
    • Dominik Kraus
    • Nadine Veit
    • Rainer Probstmeier
  • View Affiliations / Copyright

    Affiliations: Life Science Inkubator GmbH, Bonn, Germany, Oral Cell Biology Group, Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany, Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany, Neuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital of Bonn, Bonn, Germany
  • Pages: 2587-2595
    |
    Published online on: September 23, 2014
       https://doi.org/10.3892/ijo.2014.2668
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Abstract

A major challenge in tumor therapy is the decrease or even the halting of cell proliferation and migration of cancerous cells. In the present study, we have analyzed the impact of a pharmacological blockade of the PI3K/Akt and MAPK/ERK1/2 signaling pathways on cell migration, proliferation and cell death in three human thyroid tumor cell lines that represent the main types of malignant thyroid carcinomas (B-CPAP, follicular; Cal-62, anaplastic; FTC-133, papillary thyroid carcinoma cells) and in which these pathways are constitutively activated. In general, pharmacological perturbation of PI3/Akt (application of MK-2206) and MEK/ERK1/2 (application of PD0325901 or U0126) signaling led to a cell line and drug-specific decrease in the proliferation and migration potential of thyroid carcinoma cells, although to a varying extent. However, one exception became apparent: in Cal-62 cells inhibition of the MEK/ERK1/2 module increased the migration rate up to 50%. This effect could be prevented by a simultaneous suppression of the PI3/Akt pathway, but also by application of the multiple kinase inhibitor sorafenib, a treatment that did not change the activation state of Akt. Thus, a pharmacological perturbation of canonical signaling pathways in thyroid carcinoma may induce drug-dependent yin-yang effects that are characterized by a simultaneous suppression of one (i.e., proliferation) and the activation of another (i.e., migration) cellular process. The appearance of such phenomena should be taken into account when therapy plans are established.
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Copy and paste a formatted citation
Spandidos Publications style
Glassmann A, Winter J, Kraus D, Veit N and Probstmeier R: Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments. Int J Oncol 45: 2587-2595, 2014.
APA
Glassmann, A., Winter, J., Kraus, D., Veit, N., & Probstmeier, R. (2014). Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments. International Journal of Oncology, 45, 2587-2595. https://doi.org/10.3892/ijo.2014.2668
MLA
Glassmann, A., Winter, J., Kraus, D., Veit, N., Probstmeier, R."Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments". International Journal of Oncology 45.6 (2014): 2587-2595.
Chicago
Glassmann, A., Winter, J., Kraus, D., Veit, N., Probstmeier, R."Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments". International Journal of Oncology 45, no. 6 (2014): 2587-2595. https://doi.org/10.3892/ijo.2014.2668
Copy and paste a formatted citation
x
Spandidos Publications style
Glassmann A, Winter J, Kraus D, Veit N and Probstmeier R: Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments. Int J Oncol 45: 2587-2595, 2014.
APA
Glassmann, A., Winter, J., Kraus, D., Veit, N., & Probstmeier, R. (2014). Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments. International Journal of Oncology, 45, 2587-2595. https://doi.org/10.3892/ijo.2014.2668
MLA
Glassmann, A., Winter, J., Kraus, D., Veit, N., Probstmeier, R."Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments". International Journal of Oncology 45.6 (2014): 2587-2595.
Chicago
Glassmann, A., Winter, J., Kraus, D., Veit, N., Probstmeier, R."Pharmacological suppression of the Ras/MAPK pathway in thyroid carcinoma cells can provoke opposite effects on cell migration and proliferation: The appearance of yin-yang effects and the need of combinatorial treatments". International Journal of Oncology 45, no. 6 (2014): 2587-2595. https://doi.org/10.3892/ijo.2014.2668
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