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Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer

  • Authors:
    • Christina Lönnroth
    • Marianne Andersson
    • Annika G. Asting
    • Svante Nordgren
    • Kent Lundholm
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Surgical Metabolic Research Laboratory at Lundberg Laboratory for Cancer Research, Sahlgrenska University Hospital, University of Gothenburg, SE 413 45 Gothenburg, Sweden
    Copyright: © Lönnroth et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2208-2220
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    Published online on: September 30, 2014
       https://doi.org/10.3892/ijo.2014.2686
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Abstract

Preclinical data, and an increasing list of clinical investigations, show anti‑inflammatory agents to favourably influence the biology of colorectal tumor. We have earlier reported on re‑expression of activated immune cells after three days preoperative treatment of patients with colorectal carcinoma, randomized to receive oral NSAID (indomethacin or celebrex). Antisecretory prophylaxis (esomeprasol) was provided to all patients and served as sham treatment. Concomittant to MHC locus activation, Prominin1/CD133, a marker associated with stemness and poor prognosis in several solid tumors, was downregulated. The aim of the present study was to evaluate expression of additional regulators belonging to the stem cell niche, OCT4, SOX2 and BMP7, as well as some microRNAs, reported to act as tumor suppressors or oncomiRs. Peroperative tumor biopsies were analyzed by microarrays, quantitative real‑time PCR and immunohistochemistry (IHC). The stem cell master regulator SOX2 was increased by NSAIDs (p<0.01), as well as the tumor suppressor miR‑630 (p<0.01), while BMP7, a marker for poor prognosis in CRC, was downregulated by NSAID (indomethacin, p<0.02). The upregulation of SOX2, but not of its heterodimer binding partner OCT4, could imply a negative feed‑back loop, with a switch‑off for stemness preservation of tumor cells. This is supported by the overall evaluation of gene expression profiles with subsequent events, indicating less aggressive tumors following NSAID treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Lönnroth C, Andersson M, Asting AG, Nordgren S and Lundholm K: Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer. Int J Oncol 45: 2208-2220, 2014.
APA
Lönnroth, C., Andersson, M., Asting, A.G., Nordgren, S., & Lundholm, K. (2014). Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer. International Journal of Oncology, 45, 2208-2220. https://doi.org/10.3892/ijo.2014.2686
MLA
Lönnroth, C., Andersson, M., Asting, A. G., Nordgren, S., Lundholm, K."Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer". International Journal of Oncology 45.6 (2014): 2208-2220.
Chicago
Lönnroth, C., Andersson, M., Asting, A. G., Nordgren, S., Lundholm, K."Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer". International Journal of Oncology 45, no. 6 (2014): 2208-2220. https://doi.org/10.3892/ijo.2014.2686
Copy and paste a formatted citation
x
Spandidos Publications style
Lönnroth C, Andersson M, Asting AG, Nordgren S and Lundholm K: Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer. Int J Oncol 45: 2208-2220, 2014.
APA
Lönnroth, C., Andersson, M., Asting, A.G., Nordgren, S., & Lundholm, K. (2014). Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer. International Journal of Oncology, 45, 2208-2220. https://doi.org/10.3892/ijo.2014.2686
MLA
Lönnroth, C., Andersson, M., Asting, A. G., Nordgren, S., Lundholm, K."Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer". International Journal of Oncology 45.6 (2014): 2208-2220.
Chicago
Lönnroth, C., Andersson, M., Asting, A. G., Nordgren, S., Lundholm, K."Preoperative low dose NSAID treatment influences the genes for stemness, growth, invasion and metastasis in colorectal cancer". International Journal of Oncology 45, no. 6 (2014): 2208-2220. https://doi.org/10.3892/ijo.2014.2686
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