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6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation

  • Authors:
    • Yong-Chun Li
    • Bong-Hee Kim
    • Soon-Chang Cho
    • Mi-Ae Bang
    • Sunmin Kim
    • Dae-Hun Park
  • View Affiliations / Copyright

    Affiliations: Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Republic of Korea, Chungnam National University, Yuseong, Daejeon 305-764, Republic of Korea, Research Institute, NaturePureKorea Inc., Damyang 517-803, Republic of Korea, Food Industry Development Team, Jeonnam Biofood Technology Center, Naju, Republic of Korea
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 578-586
    |
    Published online on: November 7, 2014
       https://doi.org/10.3892/ijo.2014.2739
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Abstract

We evaluated the cytostatic effect of 6,7-di-O-acetyl­sinococuline (FK-3000) isolated from Stephania delavayi Diels. against breast carcinoma cell lines MDA-MB‑231 and MCF-7. FK-3000 suppressed CDC25B phosphorylation directly and indirectly via p38 MAPK phosphorylation. CDC25B dephosphorylation decreased levels of cyclin B and phospho-CDC-2, and ultimately induced cell cycle arrest at the G2/M phase. The p38 MAPK inhibitor, SB 239063 blocked FK-3000-induced p38 MAPK phosphorylation and nuclear accumulation, but did not completely rescue cell death. Conclusively FK-3000 exerts its antiproliferative effect through two pathways: i) G2/M cell cycle arrest via downregulation of cyclin B and phospho-CDC2 by p38 MAPK phosphorylation and CDC25B dephosphorylation, and ii) p38 MAPK-independent induction of apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Kim B, Cho S, Bang M, Kim S and Park D: 6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation. Int J Oncol 46: 578-586, 2015.
APA
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., & Park, D. (2015). 6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation. International Journal of Oncology, 46, 578-586. https://doi.org/10.3892/ijo.2014.2739
MLA
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., Park, D."6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation". International Journal of Oncology 46.2 (2015): 578-586.
Chicago
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., Park, D."6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation". International Journal of Oncology 46, no. 2 (2015): 578-586. https://doi.org/10.3892/ijo.2014.2739
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Kim B, Cho S, Bang M, Kim S and Park D: 6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation. Int J Oncol 46: 578-586, 2015.
APA
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., & Park, D. (2015). 6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation. International Journal of Oncology, 46, 578-586. https://doi.org/10.3892/ijo.2014.2739
MLA
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., Park, D."6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation". International Journal of Oncology 46.2 (2015): 578-586.
Chicago
Li, Y., Kim, B., Cho, S., Bang, M., Kim, S., Park, D."6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation". International Journal of Oncology 46, no. 2 (2015): 578-586. https://doi.org/10.3892/ijo.2014.2739
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