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Article

Label-free and amplification-free miR-124 detection in human cells

  • Authors:
    • Kristyna Smerkova
    • Kristyna Hudcova
    • Veronika Vlahova
    • Marketa Vaculovicova
    • Vladimir Pekarik
    • Michal Masarik
    • Vojtech Adam
    • Rene Kizek
  • View Affiliations / Copyright

    Affiliations: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, CZ-613 00 Brno, Czech Republic, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, CZ-625 00 Brno, Czech Republic, Central European Institute of Technology, Brno University of Technology, CZ-616 00 Brno, Czech Republic
  • Pages: 871-877
    |
    Published online on: November 17, 2014
       https://doi.org/10.3892/ijo.2014.2756
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Abstract

MicroRNAs (miRNAs) are becoming a very important group of molecules especially since their connection to numerous diseases has been revealed. The potential in gene therapy as well as in diagnostics is being widely investigated leading to the demand of sensitive, selective and simple methods of isolation and detection. The combined advantages of magnetic particle-based separation with sensitive electrochemical detection may offer a very valuable tool for these purposes. In this study, the miR‑124 was targeted as an example analyte for development and optimization of the isolation procedure coupled to the electrochemical detection. The sensitivity of the method was demonstrated by the limit of detection at the level of nanomolar concentration (4 nM). To verify the applicability of the procedure to the real samples, miR‑124 was isolated from the human embryonic kidney cells naturally expressing this miRNA molecule and the results were compared to the amount of miR‑124 isolated from the cells transfected by the pENTR-miR‑124 plasmid leading to the overexpression of miR‑124.
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1 

Lee RC, Feinbaum RL and Ambros V: The c-elegans heterochronic gene lin-4 encodes small rnas with antisense complementarity to lin-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

2 

Winter J, Jung S, Keller S, Gregory RI and Diederichs S: Many roads to maturity: microRNA biogenesis pathways and their regulation. Nat Cell Biol. 11:228–234. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Krol J, Loedige I and Filipowicz W: The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet. 11:597–610. 2010.PubMed/NCBI

4 

Mirnezami AHF, Pickard K, Zhang L, Primrose JN and Packham G: MicroRNAs: key players in carcinogenesis and novel therapeutic targets. Eur J Surg Oncol. 35:339–347. 2009. View Article : Google Scholar

5 

Ruan K, Fang XG and Ouyang GL: MicroRNAs: novel regulators in the hallmarks of human cancer. Cancer Lett. 285:116–126. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Paranjape T, Slack FJ and Weidhaas JB: MicroRNAs: tools for cancer diagnostics. Gut. 58:1546–1554. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Hannafon BN, Sebastiani P, de las Morenas A, Lu JN and Rosenberg CL: Expression of microRNA and their gene targets are dysregulated in preinvasive breast cancer. Breast Cancer Res. 13:1–14. 2011. View Article : Google Scholar

8 

Sieuwerts AM, Mostert B, Bolt-de Vries J, et al: mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients. Clin Cancer Res. 17:3600–3618. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Furuta M, Kozaki KI, Tanaka S, Arii S, Imoto I and Inazawa J: miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis. 31:766–776. 2010. View Article : Google Scholar

10 

Agirre X, Vilas-Zornoza A, Jimenez-Velasco A, et al: Epigenetic silencing of the tumor suppressor microRNA Hsa-miR-124a regulates CDK6 expression and confers a poor prognosis in acute lymphoblastic leukemia. Cancer Res. 69:4443–4453. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Shi XB, Xue L, Ma AH, et al: Tumor suppressive miR-124 targets androgen receptor and inhibits proliferation of prostate cancer cells. Oncogene. 32:4130–4138. 2013. View Article : Google Scholar

12 

Makeyev EV, Zhang JW, Carrasco MA and Maniatis T: The microRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing. Mol Cell. 27:435–448. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Cheng LC, Pastrana E, Tavazoie M and Doetsch F: miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche. Nat Neurosci. 12:399–408. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Lee MR, Kim JS and Kim K-S: miR-124a is important for migratory cell fate transition during gastrulation of human embryonic stem cells. Stem Cells. 28:1550–1559. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Bernardo BC, Charchar FJ, Lin RCY and McMullen JR: A microRNA guide for clinicians and basic scientists: background and experimental techniques. Heart Lung Circ. 21:131–142. 2012. View Article : Google Scholar

16 

Li W and Ruan KC: MicroRNA detection by microarray. Anal Bioanal Chem. 394:1117–1124. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Obernosterer G, Martinez J and Alenius M: Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections. Nat Protoc. 2:1508–1514. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Pena JTG, Sohn-Lee C, Rouhanifard SH, et al: miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat Methods. 6:139–141. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Schmittgen TD, Lee EJ, Jiang JM, et al: Real-time PCR quantification of precursor and mature microRNA. Methods. 44:31–38. 2008. View Article : Google Scholar

20 

Streit S, Michalski CW, Erkan M, Kleeff J and Friess H: Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues. Nat Protoc. 4:37–43. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Valoczi A, Hornyik C, Varga N, Burgyan J, Kauppinen S and Havelda Z: Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res. 32:1–7. 2004. View Article : Google Scholar

22 

Wang XW: A PCR-based platform for microRNA expression profiling studies. RNA. 15:716–723. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Palecek E and Bartosik M: Electrochemistry of nucleic acids. Chem Rev. 112:3427–3481. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Palecek E: Oscillographic polarography of highly polymerized deoxyribonucleic acid. Nature. 188:656–657. 1960. View Article : Google Scholar : PubMed/NCBI

25 

Hynek D, Prasek J, Koudelka P, et al: Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes (Review). Curr Phys Chem. 1:299–324. 2011. View Article : Google Scholar

26 

Osteryoung JG and Osteryoung RA: Square-wave voltammetry. Anal Chem. 57:A101–A110. 1985. View Article : Google Scholar

27 

Gao ZQ and Yang ZC: Detection of microRNAs using electrocatalytic nanoparticle tags. Anal Chem. 78:1470–1477. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Peng YF and Gao ZQ: Amplified detection of microRNA based on ruthenium oxide nanoparticle-initiated deposition of an insulating film. Ana Chem. 83:820–827. 2011. View Article : Google Scholar

29 

Peng YL, Jiang JH and Yu RQ: A sensitive electrochemical biosensor for microRNA detection based on streptavidin-gold nanoparticles and enzymatic amplification. Anal Methods. 6:2889–2893. 2014. View Article : Google Scholar

30 

Bettazzi F, Hamid-Asl E, Esposito CL, et al: Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay. Anal Bioanal Chem. 405:1025–1034. 2013. View Article : Google Scholar

31 

Bartosik M, Hrstka R, Palecek E and Vojtesek B: Magnetic bead-based hybridization assay for electrochemical detection of microRNA. Anal Chim Acta. 813:35–40. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Zhang GJ, Chua JH, Chee RE, Agarwal A and Wong SM: Label-free direct detection of MiRNAs with silicon nanowire biosensors. Biosens Bioelectron. 24:2504–2508. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Dong HF, Jin S, Ju HX, et al: Trace and label-free microRNA detection using oligonucleotide encapsulated silver nanoclusters as probes. Anal Chem. 84:8670–8674. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Yin HS, Zhou YL, Zhang HX, Meng XM and Ai SY: Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system. Biosens Bioelectron. 33:247–253. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Tran HV, Piro B, Reisberg S, Tran LD, Duc HT and Pham MC: Label-free and reagentless electrochemical detection of microRNAs using a conducting polymer nanostructured by carbon nanotubes: Application to prostate cancer biomarker miR-141. Biosens Bioelectron. 49:164–169. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Huska D, Hubalek J, Adam V, et al: Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection. Talanta. 79:402–411. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Hynek D, Krejcova L, Zitka O, et al: Electrochemical study of doxorubicin interaction with different sequences of single stranded oligonucleotides, part I. Int J Electrochem Sci. 7:13–33. 2012.

38 

Long GL and Winefordner JD: Limit of detection. Anal Chem. 55:A712–A724. 1983.

39 

Causon R: Validation of chromatographic methods in biomedical analysis - viewpoint and discussion. J Chromatogr B. 689:175–180. 1997. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Smerkova K, Hudcova K, Vlahova V, Vaculovicova M, Pekarik V, Masarik M, Adam V and Kizek R: Label-free and amplification-free miR-124 detection in human cells. Int J Oncol 46: 871-877, 2015.
APA
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M. ... Kizek, R. (2015). Label-free and amplification-free miR-124 detection in human cells. International Journal of Oncology, 46, 871-877. https://doi.org/10.3892/ijo.2014.2756
MLA
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M., Adam, V., Kizek, R."Label-free and amplification-free miR-124 detection in human cells". International Journal of Oncology 46.2 (2015): 871-877.
Chicago
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M., Adam, V., Kizek, R."Label-free and amplification-free miR-124 detection in human cells". International Journal of Oncology 46, no. 2 (2015): 871-877. https://doi.org/10.3892/ijo.2014.2756
Copy and paste a formatted citation
x
Spandidos Publications style
Smerkova K, Hudcova K, Vlahova V, Vaculovicova M, Pekarik V, Masarik M, Adam V and Kizek R: Label-free and amplification-free miR-124 detection in human cells. Int J Oncol 46: 871-877, 2015.
APA
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M. ... Kizek, R. (2015). Label-free and amplification-free miR-124 detection in human cells. International Journal of Oncology, 46, 871-877. https://doi.org/10.3892/ijo.2014.2756
MLA
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M., Adam, V., Kizek, R."Label-free and amplification-free miR-124 detection in human cells". International Journal of Oncology 46.2 (2015): 871-877.
Chicago
Smerkova, K., Hudcova, K., Vlahova, V., Vaculovicova, M., Pekarik, V., Masarik, M., Adam, V., Kizek, R."Label-free and amplification-free miR-124 detection in human cells". International Journal of Oncology 46, no. 2 (2015): 871-877. https://doi.org/10.3892/ijo.2014.2756
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