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Long non-coding RNA HOTAIR is associated with human cervical cancer progression

  • Authors:
    • Hee Jung Kim
    • Dae Woo Lee
    • Ga Won Yim
    • Eun Ji Nam
    • Sunghoon Kim
    • Sang Wun Kim
    • Young Tae Kim
  • View Affiliations / Copyright

    Affiliations: Institute of Women's Life Medical Science, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Obstetrics and Gynecology, Yonsei University Graduate School, Seoul, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 521-530
    |
    Published online on: November 17, 2014
       https://doi.org/10.3892/ijo.2014.2758
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Abstract

The functions of many long non-coding RNAs (lncRNAs) in human cancers remain to be clarified. The lncRNA Hox transcript antisense intergenic RNA (HOTAIR) has been reported to reprogram chromatin organization and promote breast and colorectal cancer metastasis, the involvement of lncRNAs in cervical cancer is just beginning to be studied. In the present study, we examined the expression and the functional role of HOTAIR in cervical cancer. HOTAIR expression was determined in cervical cancer tissues (n=111) and corresponding normal tissues (n=40) by using real-time polymerase chain reaction, and its correlation with clinical parameters and prognosis were analyzed. To determine the effect of HOTAIR knockdown and overexpression in cervical cancer cell lines, we used the CCK-8 assay, wound healing migration and matrigel invasion assay. The expression level of HOTAIR in cervical cancer tissues was higher than that in corresponding non-cancerous tissues. High HOTAIR expression correlated with lymph node metastasis, and reduced overall survival. A multivariate analysis showed that HOTAIR was a prognostic factor for predicting cervical cancer recurrence. Knockdown of HOTAIR reduced cell proliferation, migration, and invasion in cervical cancer cell lines. Moreover, HOTAIR regulated the expression of vascular endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes, which are important for cell motility and metastasis. Therefore, HOTAIR may promote tumor aggressiveness through the upregulation of VEGF and MMP-9 and EMT-related genes. These findings indicate that HOTAIR may represent a novel biomarker for predicting recurrence and prognosis and serve as a promising therapeutic target in cervical cancer.
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1 

Guttman M, Amit I, Garber M, et al: Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature. 458:223–227. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Bernstein E and Allis CD: RNA meets chromatin. Genes Dev. 19:1635–1655. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Mattick JS: The genetic signatures of noncoding RNAs. PLoS Genet. 5:e10004592009. View Article : Google Scholar : PubMed/NCBI

4 

Carthew RW and Sontheimer EJ: Origins and mechanisms of miRNAs and siRNAs. Cell. 136:642–655. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Borchert GM, Lanier W and Davidson BL: RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol. 13:1097–1101. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Pillai RS, Bhattacharyya SN and Filipowicz W: Repression of protein synthesis by miRNAs: how many mechanisms? Trends Cell Biol. 17:118–126. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Berezikov E and Plasterk RH: Camels and zebrafish, viruses and cancer: a microRNA update. Hum Mol Genet. 14:R183–R190. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Prasanth KV and Spector DL: Eukaryotic regulatory RNAs: an answer to the ‘genome complexity’ conundrum. Genes Dev. 21:11–42. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Perez DS, Hoage TR, Pritchett JR, et al: Long, abundantly expressed non-coding transcripts are altered in cancer. Hum Mol Genet. 17:642–655. 2008. View Article : Google Scholar

10 

Guttman M, Donaghey J, Carey BW, et al: lincRNAs act in the circuitry controlling pluripotency and differentiation. Nature. 477:295–300. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Rinn JL, Kertesz M, Wang JK, et al: Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell. 129:1311–1323. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Ponting CP, Oliver PL and Reik W: Evolution and functions of long noncoding RNAs. Cell. 136:629–641. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Khalil AM, Guttman M, Huarte M, et al: Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci USA. 106:11667–11672. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Hung T, Wang Y, Lin MF, et al: Extensive and coordinated transcription of noncoding RNAs within cell-cycle promoters. Nat Genet. 43:621–629. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Gibb EA, Brown CJ and Lam WL: The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 10:382011. View Article : Google Scholar : PubMed/NCBI

16 

Gupta RA, Shah N, Wang KC, et al: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 464:1071–1076. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Kim K, Jutooru I, Chadalapaka G, et al: HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene. 32:1616–1625. 2013. View Article : Google Scholar

18 

Kogo R, Shimamura T, Mimori K, et al: Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res. 71:6320–6326. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Huang L, Liao LM, Liu AW, et al: Overexpression of long noncoding RNA HOTAIR predicts a poor prognosis in patients with cervical cancer. Arch Gynecol Obstet. 290:717–723. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Kodama J, Seki N, Masahiro S, et al: Prognostic factors in stage IB–IIB cervical adenocarcinoma patients treated with radical hysterectomy and pelvic lymphadenectomy. J Surg Oncol. 101:413–417. 2010.PubMed/NCBI

22 

Noordhuis MG, Fehrmann RS, Wisman GB, et al: Involvement of the TGF-beta and beta-catenin pathways in pelvic lymph node metastasis in early-stage cervical cancer. Clin Cancer Res. 17:1317–1330. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Kang HW, Wang F, Wei Q, et al: miR-20a promotes migration and invasion by regulating TNKS2 in human cervical cancer cells. FEBS Lett. 586:897–904. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Qiang R, Wang F, Shi LY, et al: Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells. Int J Biochem Cell Biol. 43:632–641. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Liu L, Yu X, Guo X, et al: miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep. 5:753–760. 2012.

26 

Wang F, Li Y, Zhou J, et al: miR-375 is down-regulated in squamous cervical cancer and inhibits cell migration and invasion via targeting transcription factor SP1. Am J Pathol. 179:2580–2588. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Au Yeung CL, Tsang TY, Yau PL and Kwok TT: Human papillomavirus type 16 E6 induces cervical cancer cell migration through the p53/microRNA-23b/urokinase-type plasminogen activator pathway. Oncogene. 30:2401–2410. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Kim KJ, Li B, Winer J, et al: Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo. Nature. 362:841–844. 1993. View Article : Google Scholar : PubMed/NCBI

29 

Curran S and Murray GI: Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis. Eur J Cancer. 36:1621–1630. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Carninci P, Kasukawa T, Katayama S, et al: The transcriptional landscape of the mammalian genome. Science. 309:1559–1563. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Dinger ME, Amaral PP, Mercer TR, et al: Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation. Genome Res. 18:1433–1445. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Pang KC, Dinger ME, Mercer TR, et al: Genome-wide identification of long noncoding RNAs in CD8+ T cells. J Immunol. 182:7738–7748. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Hall PA and Russell SH: New perspectives on neoplasia and the RNA world. Hematol Oncol. 23:49–53. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Umlauf D, Fraser P and Nagano T: The role of long non-coding RNAs in chromatin structure and gene regulation: variations on a theme. Biol Chem. 389:323–331. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Morris KV and Vogt PK: Long antisense non-coding RNAs and their role in transcription and oncogenesis. Cell Cycle. 9:2544–2547. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Kim MK, Jo H, Kong HJ, et al: Postoperative nomogram predicting risk of recurrence after radical hysterectomy for early-stage cervical cancer. Int J Gynecol Cancer. 20:1581–1586. 2010.

37 

Soliman PT, Frumovitz M, Sun CC, et al: Radical hysterectomy: a comparison of surgical approaches after adoption of robotic surgery in gynecologic oncology. Gynecol Oncol. 123:333–336. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Biewenga P, van der Velden J, Mol BW, et al: Prognostic model for survival in patients with early stage cervical cancer. Cancer. 117:768–776. 2011. View Article : Google Scholar

39 

Wiercinska E, Naber HP, Pardali E, van der Pluijm G, van Dam H and ten Dijke P: The TGF-beta/Smad pathway induces breast cancer cell invasion through the up-regulation of matrix metalloproteinase 2 and 9 in a spheroid invasion model system. Breast Cancer Res Treat. 128:657–666. 2011. View Article : Google Scholar

40 

Burger RA: Role of vascular endothelial growth factor inhibitors in the treatment of gynecologic malignancies. J Gynecol Oncol. 21:3–11. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Geng YJ, Xie SL, Li Q, Ma J and Wang GY: Large intervening non-coding RNA HOTAIR is associated with hepatocellular carcinoma progression. J Int Med Res. 39:2119–2128. 2011. View Article : Google Scholar

42 

Xu ZY, Yu QM, Du YA, et al: Knockdown of long non-coding RNA HOTAIR suppresses tumor invasion and reverses epithelial-mesenchymal transition in gastric cancer. Int J Biol Sci. 9:587–597. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim HJ, Lee DW, Yim GW, Nam EJ, Kim S, Kim SW and Kim YT: Long non-coding RNA HOTAIR is associated with human cervical cancer progression. Int J Oncol 46: 521-530, 2015.
APA
Kim, H.J., Lee, D.W., Yim, G.W., Nam, E.J., Kim, S., Kim, S.W., & Kim, Y.T. (2015). Long non-coding RNA HOTAIR is associated with human cervical cancer progression. International Journal of Oncology, 46, 521-530. https://doi.org/10.3892/ijo.2014.2758
MLA
Kim, H. J., Lee, D. W., Yim, G. W., Nam, E. J., Kim, S., Kim, S. W., Kim, Y. T."Long non-coding RNA HOTAIR is associated with human cervical cancer progression". International Journal of Oncology 46.2 (2015): 521-530.
Chicago
Kim, H. J., Lee, D. W., Yim, G. W., Nam, E. J., Kim, S., Kim, S. W., Kim, Y. T."Long non-coding RNA HOTAIR is associated with human cervical cancer progression". International Journal of Oncology 46, no. 2 (2015): 521-530. https://doi.org/10.3892/ijo.2014.2758
Copy and paste a formatted citation
x
Spandidos Publications style
Kim HJ, Lee DW, Yim GW, Nam EJ, Kim S, Kim SW and Kim YT: Long non-coding RNA HOTAIR is associated with human cervical cancer progression. Int J Oncol 46: 521-530, 2015.
APA
Kim, H.J., Lee, D.W., Yim, G.W., Nam, E.J., Kim, S., Kim, S.W., & Kim, Y.T. (2015). Long non-coding RNA HOTAIR is associated with human cervical cancer progression. International Journal of Oncology, 46, 521-530. https://doi.org/10.3892/ijo.2014.2758
MLA
Kim, H. J., Lee, D. W., Yim, G. W., Nam, E. J., Kim, S., Kim, S. W., Kim, Y. T."Long non-coding RNA HOTAIR is associated with human cervical cancer progression". International Journal of Oncology 46.2 (2015): 521-530.
Chicago
Kim, H. J., Lee, D. W., Yim, G. W., Nam, E. J., Kim, S., Kim, S. W., Kim, Y. T."Long non-coding RNA HOTAIR is associated with human cervical cancer progression". International Journal of Oncology 46, no. 2 (2015): 521-530. https://doi.org/10.3892/ijo.2014.2758
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