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Article

SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis

  • Authors:
    • Bihui Yang
    • Li Wang
    • Xiaohua Luo
    • Liping Chen
    • Zesong Yang
    • Lin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 649-656
    |
    Published online on: November 18, 2014
       https://doi.org/10.3892/ijo.2014.2768
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Abstract

SPAG6, which is a novel cancer-testis antigen, is overexpressed in myeloid malignancies. Previously, SPAG6 was found in UPD (uniparental disomy) region of myeloid cell DNA from MDS patients and reported that SPAG6 may be a predictive marker of minimal residual disease in pediatric acute myeloid, but the biological role of SPAG6 in myeloid malignancies remains unclear. The present study was undertaken to determine the expression and functional significance of SPAG6 in malignant myeloid hematologic cell lines. A short hairpin RNA (shRNA) targeting SPAG6 was designed that could specifically inhibit SPAG6 expression at the mRNA and protein levels when introduced into the malignant myeloid hematologic cell lines SKM-1 and K562. The results from flow cytometry and CCK-8 assays showed that SPAG6 silencing inhibited the proliferation of SKM-1/K562 by inducing apoptosis. Furthermore, SPAG6 silencing resulted in activation of caspase-3, -9 and -8 and upregulated the mRNA and protein expression of p53 and PTEN. Then, we subcutaneously inoculated the monoclonal cells into NOD/SCID mice to establish xenograft models, and we found that the SPAG6-shRNA lentivirus dramatically inhibited tumor growth and increased apoptosis in vivo. These findings demonstrate that SPAG6 might have a role in malignant myeloid hematologic cell proliferation and apoptosis by regulating caspase proteins and p53, suggesting that SPAG6 may be a potential therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Yang B, Wang L, Luo X, Chen L, Yang Z and Liu L: SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. Int J Oncol 46: 649-656, 2015.
APA
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., & Liu, L. (2015). SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. International Journal of Oncology, 46, 649-656. https://doi.org/10.3892/ijo.2014.2768
MLA
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., Liu, L."SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis". International Journal of Oncology 46.2 (2015): 649-656.
Chicago
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., Liu, L."SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis". International Journal of Oncology 46, no. 2 (2015): 649-656. https://doi.org/10.3892/ijo.2014.2768
Copy and paste a formatted citation
x
Spandidos Publications style
Yang B, Wang L, Luo X, Chen L, Yang Z and Liu L: SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. Int J Oncol 46: 649-656, 2015.
APA
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., & Liu, L. (2015). SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. International Journal of Oncology, 46, 649-656. https://doi.org/10.3892/ijo.2014.2768
MLA
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., Liu, L."SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis". International Journal of Oncology 46.2 (2015): 649-656.
Chicago
Yang, B., Wang, L., Luo, X., Chen, L., Yang, Z., Liu, L."SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis". International Journal of Oncology 46, no. 2 (2015): 649-656. https://doi.org/10.3892/ijo.2014.2768
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