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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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February-2015 Volume 46 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells

  • Authors:
    • Oliver Hinsche
    • Rainer Girgert
    • Günter Emons
    • Carsten Gründker
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Georg‑August University, Göttingen 37075, Germany
  • Pages: 878-884
    |
    Published online on: November 25, 2014
       https://doi.org/10.3892/ijo.2014.2778
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Abstract

Metastasis to bone is a frequent problem of advanced breast cancer. Particularly breast cancers, which do not express estrogen receptor α (ERα) and progesterone receptor (PR) and which have no overexpression of human epidermal growth factor receptor 2 (HER2), so‑called triple‑negative breast cancers (TNBCs), are considered as very aggressive and have a poor prognosis. Recently we have shown that breast cancer cell invasion was dramatically increased when co‑cultured with MG63 osteoblast‑like cells. Using this model we have now analyzed whether estrogen receptor β (ERβ) plays a role in TNBC cell invasion in vitro. ERα and ERβ protein expression was analyzed using western blot analysis. Invasion was quantified by assessment of TNBC cell migration rate through an artificial basement membrane in a modified Boyden chamber during co‑culture with MG63 osteoblast‑like cells. The effects of ERβ agonist treatment on CXC motif chemokine receptor 4 (CXCR4) protein expression during co‑culture with MG64 cells was quantified using western blot analysis. Proliferation was measured using alamarBlue assay. TNBC cell lines HCC1806 and HCC1937 showed no ERα but high ERβ protein expression. Cell invasion of HCC1806 and HCC1937 TNBC cells was significantly increased when co‑cultured with MG63 osteoblast‑like cells. Treatment with ERβ selective estrogen agonists liquiritigenin and ERB‑041 reduced the ability to invade a reconstituted basement membrane and to migrate in response to the cellular stimulus. During co‑culture CXCR4 protein expression of TNBC cell lines HCC1806 and HCC1937 was significantly increased. Treatment with liquiritigenin resulted in a significant decrease of CXCR4 protein expression. Both ERβ agonists showed no effect on TNBC cell proliferation. Our findings suggest that ERβ plays a major role in TNBC invasion. Bone‑directed invasion can be inhibited by ERβ agonists.
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Copy and paste a formatted citation
Spandidos Publications style
Hinsche O, Girgert R, Emons G and Gründker C: Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells. Int J Oncol 46: 878-884, 2015.
APA
Hinsche, O., Girgert, R., Emons, G., & Gründker, C. (2015). Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells. International Journal of Oncology, 46, 878-884. https://doi.org/10.3892/ijo.2014.2778
MLA
Hinsche, O., Girgert, R., Emons, G., Gründker, C."Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells". International Journal of Oncology 46.2 (2015): 878-884.
Chicago
Hinsche, O., Girgert, R., Emons, G., Gründker, C."Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells". International Journal of Oncology 46, no. 2 (2015): 878-884. https://doi.org/10.3892/ijo.2014.2778
Copy and paste a formatted citation
x
Spandidos Publications style
Hinsche O, Girgert R, Emons G and Gründker C: Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells. Int J Oncol 46: 878-884, 2015.
APA
Hinsche, O., Girgert, R., Emons, G., & Gründker, C. (2015). Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells. International Journal of Oncology, 46, 878-884. https://doi.org/10.3892/ijo.2014.2778
MLA
Hinsche, O., Girgert, R., Emons, G., Gründker, C."Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells". International Journal of Oncology 46.2 (2015): 878-884.
Chicago
Hinsche, O., Girgert, R., Emons, G., Gründker, C."Estrogen receptor β selective agonists reduce invasiveness of triple‑negative breast cancer cells". International Journal of Oncology 46, no. 2 (2015): 878-884. https://doi.org/10.3892/ijo.2014.2778
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