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Article Open Access

Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule

  • Authors:
    • Hadis Honarvar
    • Javad Garousi
    • Elin Gunneriusson
    • Ingmarie Höidén-Guthenberg
    • Mohamed Altai
    • Charles Widström
    • Vladimir Tolmachev
    • Fredrik Y. Frejd
  • View Affiliations / Copyright

    Affiliations: Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden, Affibody AB, SE-17163 Solna, Sweden, Department of Hospital Physics, Uppsala University Hospital, SE-75185 Uppsala, Sweden
    Copyright: © Honarvar et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 513-520
    |
    Published online on: November 27, 2014
       https://doi.org/10.3892/ijo.2014.2782
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Abstract

Carbonic anhydrase IX (CAIX) is a transmembrane enzyme involved in regulation of tissue pH balance. In cancer, CAIX expression is associated with tumor hypoxia. CAIX is also overexpressed in renal cell carcinoma and is a molecular target for the therapeutic antibody cG250 (girentuximab). Radionuclide imaging of CAIX expression might be used for identification of patients who may benefit from cG250 therapy and from treatment strategies for hypoxic tumors. Affibody molecules are small (7 kDa) scaffold proteins having a high potential as probes for radionuclide molecular imaging. The aim of the present study was to evaluate feasibility of in vivo imaging of CAIX-expression using radiolabeled Affibody molecules. A histidine-glutamate-histidine-glutamate-histidine-glutamate (HE)3-tag-containing CAIX-binding Affibody molecule (HE)3-ZCAIX:1 was labeled with [99mTc(CO)3]+. Its binding properties were evaluated in vitro using CAIX-expressing SK-RC-52 renal carcinoma cells. 99mTc-(HE)3-ZCAIX:1 was evaluated in NMRI nu/nu mice bearing SK-RC-52 xenografts. The in vivo specificity test confirmed CAIX-mediated tumor targeting. 99mTc-(HE)3-ZCAIX:1 cleared rapidly from blood and normal tissues except for kidneys. At optimal time-point (4 h p.i.), the tumor uptake was 9.7±0.7% ID/g, and tumor-to-blood ratio was 53±10. Experimental imaging of CAIX-expressing SK-RC-52 xenografts at 4 h p.i. provided high contrast images. The use of radioiodine label for ZCAIX:1 enabled the reduction of renal uptake, but resulted in significantly lower tumor uptake and tumor-to-blood ratio. Results of the present study suggest that radiolabeled Affibody molecules are promising probes for imaging of CAIX-expression in vivo.
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1 

Parkkila S: An overview of the distribution and function of carbonic anhydrases in mammals. The Carbonic Anhydrases: New Horizons. Chegwidden WR, Carter N and Edwards Y: Birkhauser Verlag; Basel: pp. 76–93. 2000

2 

Wingo T, Tu C, Laipis PJ and Silverman DN: The catalytic properties of human carbonic anhydrase IX. Biochem Biophys Res Commun. 288:666–669. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Opavsky R, Pastorekova S, Zelnik V, et al: Human MN/CA9 gene, a novel member of the carbonic anhydrase family: structure and exon to protein domain relationships. Genomics. 33:480–487. 1996. View Article : Google Scholar : PubMed/NCBI

4 

Harris AL: Hypoxia - a key regulatory factor in tumour growth. Nat Rev Cancer. 2:38–47. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Potter C and Harris AL: Hypoxia inducible carbonic anhydrase IX, marker of tumour hypoxia, survival pathway and therapy target. Cell Cycle. 3:164–167. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Hussain SA, Ganesan R, Reynolds G, et al: Hypoxia-regulated carbonic anhydrase IX expression is associated with poor survival in patients with invasive breast cancer. Br J Cancer. 96:104–109. 2008. View Article : Google Scholar

7 

Ord JJ, Agrawal S, Thamboo TP, et al: An investigation into the prognostic significance of necrosis and hypoxia in high grade and invasive bladder cancer. J Urology. 178:677–682. 2007. View Article : Google Scholar

8 

Le QT, Kong C, Lavori PW, et al: Expression and prognostic significance of a panel of tissue hypoxia markers in head-and-neck squamous cell carcinomas. Int J Radiat Oncol Biol Phys. 69:167–175. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Driessen A, Landuyt W, Pastorekova S, et al: Expression of carbonic anhydrase IX (CAIX), a hypoxia-related protein, rather than vascular-endothelial growth factor (VEGF), a pro-angiogenic factor, correlates with an extremely poor prognosis in esophageal and gastric adenocarcinomas. Ann Surg. 243:334–340. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Swinson DE, Jones JL, Richardson D, et al: Carbonic anhydrase IX expression, a novel surrogate marker of tumor hypoxia, is associated with a poor prognosis in non-small-cell lung cancer. J Clin Oncol. 21:473–482. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Robertson N, Potter C and Harris AL: Role of carbonic anhydrase IX in human tumor cell growth, survival, and invasion. Cancer Res. 64:6160–6165. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Brizel DM, Dodge RK, Clough RW and Dewhirst MW: Oxygenation of head and neck cancer: changes during radiotherapy and impact on treatment outcome. Radiother Oncol. 53:113–117. 1999. View Article : Google Scholar

13 

Hockel M, Schlenger K, Aral B, et al: Association between tumor hypoxia and malignant progression in advanced cancer of the uterine cervix. Cancer Res. 56:4509–4515. 1996.PubMed/NCBI

14 

Zeng L, Ou G, Itasaka S, et al: TS-1 enhances the effect of radiotherapy by suppressing radiation-induced hypoxia-inducible factor-1 activation and inducing endothelial cell apoptosis. Cancer Sci. 99:2327–2335. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Zaffaroni N, Fiorentini G and De Giorgi U: Hyperthermia and hypoxia: new developments in anticancer chemotherapy. Eur J Surg Oncol. 27:340–342. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Yamazaki Y, Kunimoto S and Ikeda D: Rakicidin A: a hypoxia-selective cytotoxin. Biol Pharm Bull. 30:261–265. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Raleigh JA, Dewhirst MW and Thrall DE: Measuring tumor hypoxia. Semin Radiat Oncol. 6:37–45. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Mees G, Dierckx R, Vangestel C and Van de Wiele C: Molecular imaging of hypoxia with radiolabelled agents. Eur J Nucl Med Mol Imaging. 36:1674–1686. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Machulla HJ: Imaging of Hypoxia-Tracer Developments. Kluwer Academic Publishers; Dordrecht: pp. 1–18. 1999

20 

Wiebe LI and McEwan AJB: Scintigraphic imaging of focal hypoxic tissue: development and clinical applications of 123I-IAZA. Braz Arch Biol Technol. 45:89–102. 2002. View Article : Google Scholar

21 

Wiebe LI: PET radiopharmaceuticals for metabolic imaging in oncology. PET and Molecular Imaging: State of the Art and Future Perspectives. Tamaki Y and Kuge Y: (International Congress Series 1264C). Elsevier; Amsterdam: pp. 53–76. 2003

22 

Graham MM, Peterson LM, Link JM, et al: Fluorine-18-fluoromisonidazole radiation dosimetry in imaging studies. J Nucl Med. 38:1631–1636. 1997.PubMed/NCBI

23 

Doss M, Zhang JJ, Bélanger MJ, et al: Biodistribution and radiation dosimetry of the hypoxia marker 18F-HX4 in monkeys and humans determined by using whole-body PET/CT. Nucl Med Commun. 31:1016–1024. 2010.PubMed/NCBI

24 

Brouwers AH, Buijs WC, Mulders PF, et al: Radioimmunotherapy with [131I]cG250 in patients with metastasized renal cell cancer: dosimetric analysis and immunologic response. Clin Cancer Res. 11:7178–7186. 2005. View Article : Google Scholar

25 

Li Y, Wang H, Oosterwijk E, et al: Antibody-specific detection of CAIX in breast and prostate cancers. Biochem Biophys Res Commun. 28:488–492. 2009. View Article : Google Scholar

26 

Oosterwijk-Wakka JC, Boerman OC, Peter FA, Oosterwijk M and Oosterwijk E: Application of monoclonal antibody G250 recognizing carbonic anhydrase IX in renal cell carcinoma. Int J Mol Sci. 14:11402–11423. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Steffens MG, Boerman OC, Oyen WJG, et al: MN/CA IX/G250 as a potential target for immunotherapy of renal cell carcinomas. Br J Cancer. 81:741–746. 1999. View Article : Google Scholar

28 

Kerbel R and Folkman J: Clinical translation of angiogenesis inhibitors. Nat Rev Cancer. 2:727–739. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Povoski SP, Hall NC, Murrey DA Jr, et al: Multimodal imaging and detection strategy with 124 I-Labeled chimeric monoclonal antibody cG250 for accurate localization and confirmation of extent of disease during laparoscopic and open surgical resection of clear cell renal cell carcinoma. Surg Innov. 20:59–69. 2013. View Article : Google Scholar :

30 

Ahlskog JK, Schliemann C, Mårlind J, et al: Human monoclonal antibodies targeting carbonic anhydrase IX for the molecular imaging of hypoxic regions in solid tumours. Br J Cancer. 101:645–657. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Carlin S, Khan N, Ku T, et al: Molecular targeting of carbonic anhydrase IX in mice with hypoxic HT29 colorectal tumor xenografts. PLoS One. 5:e108572010. View Article : Google Scholar : PubMed/NCBI

32 

Hoeben BA, Kaanders JH, Franssen GM, et al: PET of hypoxia with 89Zr-labeled cG250-F(ab′)2 in head and neck tumors. J Nucl Med. 51:1076–1083. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Askoxylakis V, Garcia-Boy R, Rana S, et al: A new peptide ligand for targeting human carbonic anhydrase IX, identified through the phage display technology. PLoS One. 5:e159622010. View Article : Google Scholar

34 

Löfblom J, Feldwisch J, Tolmachev V, et al: Affibody molecules: engineered proteins for therapeutic, diagnostic and biotechnological applications. FEBS Lett. 584:2670–2680. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Nygren PÅ: Alternative binding proteins: affibody binding proteins developed from a small three-helix bundle scaffold. FEBS J. 275:2668–2676. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Orlova A, Magnusson M, Eriksson TL, et al: Tumor imaging using a picomolar affinity HER2 binding affibody molecule. Cancer Res. 66:4339–4348. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Ahlgren S and Tolmachev V: Radionuclide molecular imaging using Affibody molecules. Curr Pharm Biotechnol. 11:581–589. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Baum RP, Prasad V, Muller D, et al: Molecular imaging of HER2-expressing malignant tumors in breast cancer patients using synthetic 111In- or 68Ga-labeled affibody molecules. J Nucl Med. 51:892–897. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Sörensen J, Sandberg D, Sandström M, et al: First-in-human molecular imaging of HER2 expression in breast cancer metastases using the 111In-ABY-025 affibody molecule. J Nucl Med. 55:730–735. 2014. View Article : Google Scholar

40 

Tolmachev V, Hofstrom C, Malmberg J, et al: HEHEHE-tagged affibody molecules may be purified by IMAC, are conveniently labeled with [99mTc(CO)3]+ and show improved biodistribution. Bioconjug Chem. 21:2013–2022. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Orlova A, Wållberg H, Stone-Elander S and Tolmachev V: On the selection of a tracer for PET imaging of HER2-expressing tumors: direct comparison of a 124I-labeled Affibody molecule and trastuzumab in a murine xenograft model. J Nucl Med. 50:417–425. 2009. View Article : Google Scholar : PubMed/NCBI

42 

Björkelund H, Gedda L, Barta P, Malmqvist M and Andersson K: Gefitinib induces epidermal growth factor receptor dimers which alters the interaction characteristics with 125I-EGF. PLoS One. 6:e247392011. View Article : Google Scholar

43 

Wållberg H and Orlova A: Slow internalization of anti-HER2 synthetic affibody monomer 111In-DOTA-ZHER2:342-pep2: implications for development of labeled tracers. Cancer Biother Radiopharm. 23:435–442. 2008. View Article : Google Scholar

44 

Kramer-Marek G, Kiesewetter DO, Martiniova L, Jagoda E, Lee SB and Capala J: [18F]FBEM-ZHER2:342-Affibody molecule-a new molecular tracer for in vivo monitoring of HER2 expression by positron emission tomography. Eur J Nucl Med Mol Imaging. 35:1008–1018. 2008. View Article : Google Scholar

45 

Brouwers AH, van Eerd JE, Frielink C, Oosterwijk E, Oyen WJ, Corstens FH and Boerman OC: Optimization of radioimmunotherapy of renal cell carcinoma: labeling of monoclonal antibody cG250 with 131I, 90Y, 177Lu, or 186Re. J Nucl Med. 45:327–337. 2004.PubMed/NCBI

46 

Lawrentschuk N, Lee FT, Jones G, Rigopoulos A, Mountain A, et al: Investigation of hypoxia and carbonic anhydrase IX expression in a renal cell carcinoma xenograft model with oxygen tension measurements and 124I-cG250 PET/CT. Urol Oncol. 29:411–420. 2011. View Article : Google Scholar

47 

Akurathi V, Dubois L, Celen S, et al: Development and biological evaluation of 99mTc-sulfonamide derivatives for in vivo visualization of CA IX as surrogate tumor hypoxia markers. Eur J Med Chem. 71:374–384. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Honarvar H, Garousi J, Gunneriusson E, Höidén-Guthenberg I, Altai M, Widström C, Tolmachev V and Frejd FY: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule. Int J Oncol 46: 513-520, 2015.
APA
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C. ... Frejd, F.Y. (2015). Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule. International Journal of Oncology, 46, 513-520. https://doi.org/10.3892/ijo.2014.2782
MLA
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C., Tolmachev, V., Frejd, F. Y."Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule". International Journal of Oncology 46.2 (2015): 513-520.
Chicago
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C., Tolmachev, V., Frejd, F. Y."Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule". International Journal of Oncology 46, no. 2 (2015): 513-520. https://doi.org/10.3892/ijo.2014.2782
Copy and paste a formatted citation
x
Spandidos Publications style
Honarvar H, Garousi J, Gunneriusson E, Höidén-Guthenberg I, Altai M, Widström C, Tolmachev V and Frejd FY: Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule. Int J Oncol 46: 513-520, 2015.
APA
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C. ... Frejd, F.Y. (2015). Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule. International Journal of Oncology, 46, 513-520. https://doi.org/10.3892/ijo.2014.2782
MLA
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C., Tolmachev, V., Frejd, F. Y."Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule". International Journal of Oncology 46.2 (2015): 513-520.
Chicago
Honarvar, H., Garousi, J., Gunneriusson, E., Höidén-Guthenberg, I., Altai, M., Widström, C., Tolmachev, V., Frejd, F. Y."Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule". International Journal of Oncology 46, no. 2 (2015): 513-520. https://doi.org/10.3892/ijo.2014.2782
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