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Article

CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma

  • Authors:
    • Nao Takano
    • Mitsuhiro Hishida
    • Yoshikuni Inokawa
    • Masamichi Hayashi
    • Mitsuro Kanda
    • Yoko Nishikawa
    • Naoki Iwata
    • Daisuke Kobayashi
    • Chie Tanaka
    • Suguru Yamada
    • Goro Nakayama
    • Tsutomu Fujii
    • Hiroyuki Sugimoto
    • Masahiko Koike
    • Michitaka Fujiwara
    • Yasuhiro Kodera
    • Shuji Nomoto
  • View Affiliations / Copyright

    Affiliations: Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya 466-8550, Japan, Department of Surgery, Aichi-Gakuin University School of Dentistry, Chikusa-ku, Nagoya 464-8651, Japan
  • Pages: 1963-1970
    |
    Published online on: February 11, 2015
       https://doi.org/10.3892/ijo.2015.2892
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Abstract

Hepatocellular carcinoma (HCC) has a high likelihood of recurrence and a poor prognosis. To detect cancer-related genes of HCC, we developed a new technique: triple combination array analysis, consisting of a methylation array, a gene expression array and a single nucleotide polymorphism array. A surgical specimen obtained from a 68-year-old female HCC patient was analyzed using triple combination array, which identified cyclin J (CCNJ) as a candidate cancer-related gene of HCC. Subsequently, samples from 85 HCC patients were evaluated for CCNJ promoter hypermethylation and expression status using methylation-specific PCR (MSP) and quantitative reverse transcriptase RT-PCR, respectively. CCNJ was found to be hypermethylated (methylation value, 0.906; range, 0-1.0) in cancer tissue, compared with adjacent non-cancerous tissue (0.112) using a methylation array. MSP revealed that CCNJ was hypermethylated in 67 (78.8%) of the tumor samples. CCNJ expression was significantly decreased in cases with hypermethylation (P<0.0001). Furthermore, cases with both promoter hypermethylation and decreased expression of CCNJ in the tumor tissue had a worse overall survival than the other cases (P=0.0383). In conclusion, our results indicated that CCNJ could be a novel prognostic marker of HCC, and this study indicated that triple combination array analysis was effective in detecting new tumor-related genes and their mechanisms.
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Copy and paste a formatted citation
Spandidos Publications style
Takano N, Hishida M, Inokawa Y, Hayashi M, Kanda M, Nishikawa Y, Iwata N, Kobayashi D, Tanaka C, Yamada S, Yamada S, et al: CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma. Int J Oncol 46: 1963-1970, 2015.
APA
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y. ... Nomoto, S. (2015). CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma. International Journal of Oncology, 46, 1963-1970. https://doi.org/10.3892/ijo.2015.2892
MLA
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y., Iwata, N., Kobayashi, D., Tanaka, C., Yamada, S., Nakayama, G., Fujii, T., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y., Nomoto, S."CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma". International Journal of Oncology 46.5 (2015): 1963-1970.
Chicago
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y., Iwata, N., Kobayashi, D., Tanaka, C., Yamada, S., Nakayama, G., Fujii, T., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y., Nomoto, S."CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma". International Journal of Oncology 46, no. 5 (2015): 1963-1970. https://doi.org/10.3892/ijo.2015.2892
Copy and paste a formatted citation
x
Spandidos Publications style
Takano N, Hishida M, Inokawa Y, Hayashi M, Kanda M, Nishikawa Y, Iwata N, Kobayashi D, Tanaka C, Yamada S, Yamada S, et al: CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma. Int J Oncol 46: 1963-1970, 2015.
APA
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y. ... Nomoto, S. (2015). CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma. International Journal of Oncology, 46, 1963-1970. https://doi.org/10.3892/ijo.2015.2892
MLA
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y., Iwata, N., Kobayashi, D., Tanaka, C., Yamada, S., Nakayama, G., Fujii, T., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y., Nomoto, S."CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma". International Journal of Oncology 46.5 (2015): 1963-1970.
Chicago
Takano, N., Hishida, M., Inokawa, Y., Hayashi, M., Kanda, M., Nishikawa, Y., Iwata, N., Kobayashi, D., Tanaka, C., Yamada, S., Nakayama, G., Fujii, T., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y., Nomoto, S."CCNJ detected by triple combination array analysis as a tumor-related gene of hepatocellular carcinoma". International Journal of Oncology 46, no. 5 (2015): 1963-1970. https://doi.org/10.3892/ijo.2015.2892
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