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Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells

  • Authors:
    • Alessandro Salvi
    • Italia Bongarzone
    • Lia Ferrari
    • Edoardo Abeni
    • Bruna Arici
    • Maida De Bortoli
    • Sabrina Scuri
    • Daniela Bonini
    • Ilaria Grossi
    • Anna Benetti
    • Gianluca Baiocchi
    • Nazario Portolani
    • Giuseppina De Petro
  • View Affiliations / Copyright

    Affiliations: Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy, Department of Experimental Oncology and Molecular Medicine, Proteomics Laboratory, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy, Department of Clinical and Experimental Sciences, Division of Morbid Anatomy, University of Brescia, Brescia, Italy, Department of Clinical and Experimental Sciences, Surgical Clinic, University of Brescia, Brescia, Italy
    Copyright: © Salvi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1901-1912
    |
    Published online on: March 9, 2015
       https://doi.org/10.3892/ijo.2015.2923
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Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. We have previously reported that LASP-1 is a downstream protein of the urokinase type plasminogen activator (uPA). Here we investigated the role of LASP-1 in HCC by a molecular and biological characterization of LASP-1 expression in human HCC specimens and in cultured HCC cells. We determined the LASP-1 mRNA expression levels in 55 HCC cases with different hepatic background disease. We identified 3 groups of patients with high, equal or low LASP-1 mRNA levels in HCC tissues compared to the peritumoral (PT) tissues. In particular we found that i) the HCCs displayed a higher LASP-1 mRNA level in HCC compared to PT tissues; ii) the expression levels of LASP-1 mRNA in female HCCs were significantly higher compared to male HCCs; iii) the cirrhotic HCCs displayed a higher LASP-1 mRNA. Further, the biological characterization of the ectopic LASP-1 overexpression in HCC cells, using MALDI-TOF mass spectrometer on the LASP-1 co-immunoprecipitated fractions, displayed vimentin as a novel putative partner of LASP-1. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female HCCs and cirrhotic HCCs; and that LASP1 may play its role with vimentin in HCC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Salvi A, Bongarzone I, Ferrari L, Abeni E, Arici B, De Bortoli M, Scuri S, Bonini D, Grossi I, Benetti A, Benetti A, et al: Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. Int J Oncol 46: 1901-1912, 2015.
APA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M. ... De Petro, G. (2015). Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. International Journal of Oncology, 46, 1901-1912. https://doi.org/10.3892/ijo.2015.2923
MLA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46.5 (2015): 1901-1912.
Chicago
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46, no. 5 (2015): 1901-1912. https://doi.org/10.3892/ijo.2015.2923
Copy and paste a formatted citation
x
Spandidos Publications style
Salvi A, Bongarzone I, Ferrari L, Abeni E, Arici B, De Bortoli M, Scuri S, Bonini D, Grossi I, Benetti A, Benetti A, et al: Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. Int J Oncol 46: 1901-1912, 2015.
APA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M. ... De Petro, G. (2015). Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. International Journal of Oncology, 46, 1901-1912. https://doi.org/10.3892/ijo.2015.2923
MLA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46.5 (2015): 1901-1912.
Chicago
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46, no. 5 (2015): 1901-1912. https://doi.org/10.3892/ijo.2015.2923
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