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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells

  • Authors:
    • Dao-Lu Liu
    • Ying-Jie Li
    • Dong-Hua Yang
    • Chen-Ran Wang
    • Jun Xu
    • Nan Yao
    • Xiao-Qi Zhang
    • Zhe-Sheng Chen
    • Wen-Cai Ye
    • Dong-Mei Zhang
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Jinan University, Guangzhou 510632, P.R. China, Biosample Repository, Core Facility, Fox Chase Cancer Center, Philadelphia, PA, USA, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA
  • Pages: 2029-2038
    |
    Published online on: March 12, 2015
       https://doi.org/10.3892/ijo.2015.2925
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Abstract

Chemotherapy is one of the most common therapeutic option for metastatic tumors and hematological malignancies. ABCB1-mediated multidrug resistance is the major obstacle for chemotherapy. Natural products with diversified structures are ideal source of ABCB1 modulators. Ganoderenic acid B, a lanostane-type triterpene isolated from Ganoderma lucidum, exhibited potent reversal effect on ABCB1-mediated multidrug resistance of HepG2/ADM cells to doxorubicin, vincristine and paclitaxel. Similarly, ganoderenic acid B could also significantly reverse the resistance of ABCB1-overexpressing MCF-7/ADR cells to doxorubicin. Furthermore, ganoderenic acid B notably enhanced intracellular accumulation of rhodamine-123 in HepG2/ADM cells through inhibition of its efflux. ABCB1 siRNA interference assay indicated that the reversal activity of ganoderenic acid B was dependent on ABCB1. Further mechanistic investigations found that ganoderenic acid B did not alter the expression level of ABCB1 and the activity of ABCB1 ATPase. Molecular docking model displayed that the positions of ganoderenic acid B binding to ABCB1 were different from the region of verapamil interacted with ABCB1. Collectively, ganoderenic acid B can enhance the cytotoxicity of chemotherapeutics towards ABCB1-mediated MDR cancer cells via inhibition of the transport function of ABCB1. These findings provide evidence that ganoderenic acid B has the potential to be developed into an ABCB1-mediated multidrug resistance reversal agent.
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Copy and paste a formatted citation
Spandidos Publications style
Liu D, Li Y, Yang D, Wang C, Xu J, Yao N, Zhang X, Chen Z, Ye W, Zhang D, Zhang D, et al: Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells. Int J Oncol 46: 2029-2038, 2015.
APA
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N. ... Zhang, D. (2015). Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells. International Journal of Oncology, 46, 2029-2038. https://doi.org/10.3892/ijo.2015.2925
MLA
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N., Zhang, X., Chen, Z., Ye, W., Zhang, D."Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells". International Journal of Oncology 46.5 (2015): 2029-2038.
Chicago
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N., Zhang, X., Chen, Z., Ye, W., Zhang, D."Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells". International Journal of Oncology 46, no. 5 (2015): 2029-2038. https://doi.org/10.3892/ijo.2015.2925
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, Li Y, Yang D, Wang C, Xu J, Yao N, Zhang X, Chen Z, Ye W, Zhang D, Zhang D, et al: Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells. Int J Oncol 46: 2029-2038, 2015.
APA
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N. ... Zhang, D. (2015). Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells. International Journal of Oncology, 46, 2029-2038. https://doi.org/10.3892/ijo.2015.2925
MLA
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N., Zhang, X., Chen, Z., Ye, W., Zhang, D."Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells". International Journal of Oncology 46.5 (2015): 2029-2038.
Chicago
Liu, D., Li, Y., Yang, D., Wang, C., Xu, J., Yao, N., Zhang, X., Chen, Z., Ye, W., Zhang, D."Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells". International Journal of Oncology 46, no. 5 (2015): 2029-2038. https://doi.org/10.3892/ijo.2015.2925
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