Docosahexaenoic acid inhibits the invasion of MDA‑MB‑231 breast cancer cells through upregulation of cytokeratin‑1

Blanckaert,Vincent; Kerviel,Vincent; Lépinay,Alexandra; Joubert‑Durigneux,Vanessa; Hondermarck,Hubert; Chénais,Benoît

doi:10.3892/ijo.2015.2936

Docosahexaenoic acid inhibits the invasion of MDA‑MB‑231 breast cancer cells through upregulation of cytokeratin‑1

Authors:
- Vincent Blanckaert
- Vincent Kerviel
- Alexandra Lépinay
- Vanessa Joubert‑Durigneux
- Hubert Hondermarck
- Benoît Chénais
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Affiliations: Mer, Molécules, Santé (EA2160), IUML‑FR3473 CNRS, Université du Maine, F‑72085 Le Mans, France, CERPEM, Laval Mayenne Technopole, F‑53001 Laval, France, School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia
Published online on: March 27, 2015 https://doi.org/10.3892/ijo.2015.2936
Pages: 2649-2655

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Abstract

Docosahexaenoic acid (DHA), the main member of the omega-3 essential fatty acid family, has been shown to reduce the invasion of the triple‑negative breast cancer cell line MDA‑MB‑231, but the mechanism involved remains unclear. In the present study, a proteomic approach was used to define changes in protein expression induced by DHA. Proteins from crude membrane preparations of MDA‑MB‑231 cells treated with 100 µM DHA were separated by two‑dimensional electrophoresis (2‑DE) and differentially expressed proteins were identified using MALDI‑TOF mass spectrometry. The main changes observed were the upregulation of Keratin, type Ⅱ cytoskeletal 1 (KRT1), catalase and lamin‑A/C. Immunocytochemistry analyses confirmed the increase in KRT1 induced by DHA. Furthermore, in vitro invasion assays showed that siRNA against KRT1 was able to reverse the DHA‑induced inhibition of breast cancer cell invasion. In conclusion, KRT1 is involved in the anti‑invasive activity of DHA in breast cancer cells.

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