Open Access

FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

  • Authors:
    • Hong De Xu
    • Soon-Chang Cho
    • Mi-Ae Bang
    • Chun-Sik Bae
    • Yeonshik Choi
    • Yong-Chun Li
    • Seung-Kil Lim
    • Jaegal Shim
    • Dae-Hun Park
  • View Affiliations

  • Published online on: March 27, 2015     https://doi.org/10.3892/ijo.2015.2940
  • Pages: 2309-2316
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The World Health Organization (WHO) has reported that cancer is one of the most prevalent diseases and a leading cause of death worldwide. Many anticancer drug development studies have been pursued over the last few decades and several viable drugs have been discovered, such as paclitaxel, topotecan and irinotecan. Previously, our research group uncovered the cytocidal and cytostatic effects of the plant Stephania delavayi Diels. In this study, we determined the active chemical to be 6,7-di-O-acetylsinococuline (FK-3000). The FK-3000 half maximal inhibitory concentration (IC50) in MDA-MB-231 breast carcinoma cells at 48 h was 0.52 µg/ml and it induced apoptosis in a dose- and time-dependent manner. FK-3000 suppressed NF-κB nuclear translocation, decreased NF-κB phosphorylation, and decreased COX-2 protein expression. MDA-MB-231 xenografted mice were treated with FK-3000, Taxol, or their combination for 21 days. The tumor size was smallest in the co-treatment group, indicating that FK-3000 may have a synergistic effect with Taxol. FK-3000 treatment showed no adverse effects on blood cell counts, serum protein levels, or pathology. These studies demonstrate that FK-3000, isolated from S. delavayi Diels., is a promising, pathway-specific anticancer agent that exhibits low toxicity.
View Figures
View References

Related Articles

Journal Cover

June-2015
Volume 46 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xu HD, Cho S, Bang M, Bae C, Choi Y, Li Y, Lim S, Shim J and Park D: FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression. Int J Oncol 46: 2309-2316, 2015
APA
Xu, H.D., Cho, S., Bang, M., Bae, C., Choi, Y., Li, Y. ... Park, D. (2015). FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression. International Journal of Oncology, 46, 2309-2316. https://doi.org/10.3892/ijo.2015.2940
MLA
Xu, H. D., Cho, S., Bang, M., Bae, C., Choi, Y., Li, Y., Lim, S., Shim, J., Park, D."FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression". International Journal of Oncology 46.6 (2015): 2309-2316.
Chicago
Xu, H. D., Cho, S., Bang, M., Bae, C., Choi, Y., Li, Y., Lim, S., Shim, J., Park, D."FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression". International Journal of Oncology 46, no. 6 (2015): 2309-2316. https://doi.org/10.3892/ijo.2015.2940