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Article

High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition

  • Authors:
    • Junhyun Lee
    • Shinjung Ha
    • Chan-Kwon Jung
    • Han Hong Lee
  • View Affiliations / Copyright

    Affiliations: Division of Gastrointestinal Surgery, Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • Pages: 2431-2438
    |
    Published online on: April 1, 2015
       https://doi.org/10.3892/ijo.2015.2947
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Abstract

Tumor metastases are the ultimate target in cancer therapy. In epithelial malignancies, the expression of high-mobility-group A2 (HMGA2) is associated with disease progression and the epithelial-mesenchymal transition (EMT), which is involved in the metastatic process. The present study assessed the clinical and molecular effects of HMGA2 with the malignant tissues of 170 patients with gastric cancer and gastric cancer cells expressing HMGA2. HMGA2 expression was determined using immunohistochemistry and analyzed with respect to the clinicopathological data of patients with this tumor. In the gastric cancer cell line MKN28, in which HMGA2 was knocked down by two different short-hairpin RNAs, Transwell migration and invasion assays were conducted and western blotting was used to detect the altered expression of EMT markers. In patients with gastric cancer, HMGA2 overexpression correlated with tumor progression and was indicative of a significantly worse overall survival. Migration and invasion assays using HMGA2-knocked down MKN28 cells showed a reduction in cell migration and invasion. The upregulation of E-cadherin, an epithelial marker, and the downregulation of N-cadherin, a mesenchymal marker were observed in HMGA2-knocked down cells. In addition, expression of the transcriptional factors Snail and Zeb1 and of the EMT-pathway molecule β-catenin were decreased. HMGA2 overexpression, through its relationship to EMT, thus seems to aggravate invasion and metastasis in gastric cancer. It may therefore serve as a predictive marker in determining the clinical outcome of patients with gastric cancer and offer a promising therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Lee J, Ha S, Jung C and Lee HH: High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition. Int J Oncol 46: 2431-2438, 2015.
APA
Lee, J., Ha, S., Jung, C., & Lee, H.H. (2015). High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition. International Journal of Oncology, 46, 2431-2438. https://doi.org/10.3892/ijo.2015.2947
MLA
Lee, J., Ha, S., Jung, C., Lee, H. H."High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition". International Journal of Oncology 46.6 (2015): 2431-2438.
Chicago
Lee, J., Ha, S., Jung, C., Lee, H. H."High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition". International Journal of Oncology 46, no. 6 (2015): 2431-2438. https://doi.org/10.3892/ijo.2015.2947
Copy and paste a formatted citation
x
Spandidos Publications style
Lee J, Ha S, Jung C and Lee HH: High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition. Int J Oncol 46: 2431-2438, 2015.
APA
Lee, J., Ha, S., Jung, C., & Lee, H.H. (2015). High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition. International Journal of Oncology, 46, 2431-2438. https://doi.org/10.3892/ijo.2015.2947
MLA
Lee, J., Ha, S., Jung, C., Lee, H. H."High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition". International Journal of Oncology 46.6 (2015): 2431-2438.
Chicago
Lee, J., Ha, S., Jung, C., Lee, H. H."High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition". International Journal of Oncology 46, no. 6 (2015): 2431-2438. https://doi.org/10.3892/ijo.2015.2947
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