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Article

Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells

  • Authors:
    • David Pajuelo-Reguera
    • Lukáš Alán
    • Tomáš Olejár
    • Petr Ježek
  • View Affiliations / Copyright

    Affiliations: Department of Membrane Transport Biophysics, No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Pages: 2409-2418
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    Published online on: April 6, 2015
       https://doi.org/10.3892/ijo.2015.2953
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Abstract

Dichloroacetate (DCA) is beneficial in cancer therapy because it induces apoptosis and decreases cancer growth in vitro and in vivo without affecting non-cancer cells. DCA stimulates the activity of the enzyme pyruvate dehydrogenase by inhibiting pyruvate dehydrogenase kinase. Consequently, DCA promotes oxidative phosphorylation after glycolysis. Therefore, DCA produces changes in energy metabolism that could affect the mitochondrial network and mitophagy. This investigation determined the effects of DCA treatment on mitophagy in human neuroblastoma SH-SY5Y cells. SH-SY5Y cells were cultured and distributed into 3 groups: control, NH4Cl and chloroquine. Each group was treated with DCA at 0, 5, 30 and 60 mM for 16 h. Samples were analyzed for cell viability, mtDNA copy number, mitochondrial network morphology and expression of key proteins involved in mitochondrial dynamics, such as LC3b, FIS1, OPA1, PARKIN and PINK1. In all groups, DCA caused a decrease in cell viability, an induction of autophagy in a dose-dependent manner and a decrease in Tim23, FIS1 and PARKIN protein expression, leading to profound morphological changes in the mitochondrial network resulting in shorter and more fragmented filaments. However, TFAM protein levels remained unchanged. Similarly, the mitochondrial copy number was not significantly different among the treatment groups. In conclusion, DCA induces mitophagy and remodeling of the mitochondrial network. In this remodeling, DCA induces a decrease in the expression of key proteins involved in protein degradation and mitochondrial dynamics but does not significantly affect the mtDNA density. Blocking late phase autophagy increases the effects of DCA, suggesting that autophagy protects the cell, at least partially, against DCA.
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View References

1 

Bonnet S, Archer SL, Allalunis-Turner J, Haromy A, Beaulieu C, Thompson R, Lee CT, Lopaschuk GD, Puttagunta L, Bonnet S, et al: A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell. 11:37–51. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Lin G, Hill DK, Andrejeva G, Boult JK, Troy H, Fong AC, Orton MR, Panek R, Parkes HG, Jafar M, et al: Dichloroacetate induces autophagy in colorectal cancer cells and tumours. Br J Cancer. 111:375–385. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Stacpoole PW: The pharmacology of dichloroacetate. Metabolism. 38:1124–1144. 1989. View Article : Google Scholar : PubMed/NCBI

4 

Sun RC, Fadia M, Dahlstrom JE, Parish CR, Board PG and Blackburn AC: Reversal of the glycolytic phenotype by dichloroacetate inhibits metastatic breast cancer cell growth in vitro and in vivo. Breast Cancer Res Treat. 120:253–260. 2010. View Article : Google Scholar

5 

Madhok BM, Yeluri S, Perry SL, Hughes TA and Jayne DG: Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells. Br J Cancer. 102:1746–1752. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Cao W, Yacoub S, Shiverick KT, Namiki K, Sakai Y, Porvasnik S, Urbanek C and Rosser CJ: Dichloroacetate (DCA) sensitizes both wild-type and over expressing Bcl-2 prostate cancer cells in vitro to radiation. Prostate. 68:1223–1231. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Sun RC, Board PG and Blackburn AC: Targeting metabolism with arsenic trioxide and dichloroacetate in breast cancer cells. Mol Cancer. 10:1422011. View Article : Google Scholar : PubMed/NCBI

8 

Ayyanathan K, Kesaraju S, Dawson-Scully K and Weissbach H: Combination of sulindac and dichloroacetate kills cancer cells via oxidative damage. PLoS One. 7:e399492012. View Article : Google Scholar : PubMed/NCBI

9 

Sanchez WY, McGee SL, Connor T, Mottram B, Wilkinson A, Whitehead JP, Vuckovic S and Catley L: Dichloroacetate inhibits aerobic glycolysis in multiple myeloma cells and increases sensitivity to bortezomib. Br J Cancer. 108:1624–1633. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Fantin VR, St-Pierre J and Leder P: Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance. Cancer Cell. 9:425–434. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Gaude E and Frezza C: Defects in mitochondrial metabolism and cancer. Cancer Metab. 2:102014. View Article : Google Scholar : PubMed/NCBI

12 

Weinberg F, Hamanaka R, Wheaton WW, Weinberg S, Joseph J, Lopez M, Kalyanaraman B, Mutlu GM, Budinger GR and Chandel NS: Mitochondrial metabolism and ROS generation are essential for Kras-mediated tumorigenicity. Proc Natl Acad Sci USA. 107:8788–8793. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Singleterry J, Sreedhar A and Zhao Y: Components of cancer metabolism and therapeutic interventions. Mitochondrion. 17:50–55. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Astuti D, Hart-Holden N, Latif F, Lalloo F, Black GC, Lim C, Moran A, Grossman AB, Hodgson SV, Freemont A, et al: Genetic analysis of mitochondrial complex II subunits SDHD, SDHB and SDHC in paraganglioma and phaeochromocytoma susceptibility. Clin Endocrinol (Oxf). 59:728–733. 2003. View Article : Google Scholar

15 

Wallace DC: Mitochondria and cancer. Nat Rev Cancer. 12:685–698. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Youle RJ and Karbowski M: Mitochondrial fission in apoptosis. Nat Rev Mol Cell Biol. 6:657–663. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Chen H and Chan DC: Mitochondrial dynamics - fusion, fission, movement, and mitophagy - in neurodegenerative diseases. Hum Mol Genet. 18(R2): R169–R176. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Raynaud FI, Eccles S, Clarke PA, Hayes A, Nutley B, Alix S, Henley A, Di-Stefano F, Ahmad Z, Guillard S, et al: Pharmacologic characterization of a potent inhibitor of class I phosphatidylinositide 3-kinases. Cancer Res. 67:5840–5850. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, et al: Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 8:445–544. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Biedler JL, Roffler-Tarlov S, Schachner M and Freedman LS: Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38:3751–3757. 1978.PubMed/NCBI

21 

Matthay KK: Neuroblastoma: biology and therapy. Oncology (Williston Park). 11:1857–66; discussion 1869–72, 1875. 1997.

22 

Alán L, Špaček T, Zelenka J, Tauber J, Berková Z, Zacharovová K, Saudek F and Ježek P: Assessment of mitochondrial DNA as an indicator of islet quality: An example in Goto Kakizaki rats. Transplant Proc. 43:3281–3284. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Karess RE, Hayward WS and Hanafusa H: Cellular information in the genome of recovered avian sarcoma virus directs the synthesis of transforming protein. Proc Natl Acad Sci USA. 76:3154–3158. 1979. View Article : Google Scholar : PubMed/NCBI

24 

Morton RE and Evans TA: Modification of the bicinchoninic acid protein assay to eliminate lipid interference in determining lipoprotein protein content. Anal Biochem. 204:332–334. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Paulson JR and Laemmli UK: The structure of histone-depleted metaphase chromosomes. Cell. 12:817–828. 1977. View Article : Google Scholar : PubMed/NCBI

26 

Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Scaduto RC Jr and Grotyohann LW: Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J. 76:469–477. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Stockwin LH, Yu SX, Borgel S, Hancock C, Wolfe TL, Phillips LR, Hollingshead MG and Newton DL: Sodium dichloroacetate selectively targets cells with defects in the mitochondrial ETC. Int J Cancer. 127:2510–2519. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Cuervo AM, Stefanis L, Fredenburg R, Lansbury PT and Sulzer D: Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy. Science. 305:1292–1295. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Maclean KH, Dorsey FC, Cleveland JL and Kastan MB: Targeting lysosomal degradation induces p53-dependent cell death and prevents cancer in mouse models of lymphomagenesis. J Clin Invest. 118:79–88. 2008. View Article : Google Scholar

31 

Maes H, Kuchnio A, Peric A, Moens S, Nys K, De Bock K, Quaegebeur A, Schoors S, Georgiadou M, Wouters J, et al: Tumor vessel normalization by chloroquine independent of autophagy. Cancer Cell. 26:190–206. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Stacpoole PW: The dichloroacetate dilemma: Environmental hazard versus therapeutic goldmine - both or neither? Environ Health Perspect. 119:155–158. 2011. View Article : Google Scholar :

33 

Kankotia S and Stacpoole PW: Dichloroacetate and cancer: New home for an orphan drug? Biochim Biophys Acta. 1846:617–629. 2014.PubMed/NCBI

34 

Gong F, Peng X, Sang Y, Qiu M, Luo C, He Z, Zhao X and Tong A: Dichloroacetate induces protective autophagy in LoVo cells: Involvement of cathepsin D/thioredoxin-like protein 1 and Akt-mTOR-mediated signaling. Cell Death Dis. 4:e9132013. View Article : Google Scholar : PubMed/NCBI

35 

Cheng P, Ni Z, Dai X, Wang B, Ding W, Rae Smith A, Xu L, Wu D, He F and Lian J: The novel BH-3 mimetic apogossypolone induces Beclin-1- and ROS-mediated autophagy in human hepatocellular carcinoma cells. [corrected]. Cell Death Dis. 4:e4892013. View Article : Google Scholar

36 

Wong JYY, Huggins GS, Debidda M, Munshi NC and De Vivo I: Dichloroacetate induces apoptosis in endometrial cancer cells. Gynecol Oncol. 109:394–402. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Vella S, Conti M, Tasso R, Cancedda R and Pagano A: Dichloroacetate inhibits neuroblastoma growth by specifically acting against malignant undifferentiated cells. Int J Cancer. 130:1484–1493. 2012. View Article : Google Scholar

38 

Cai Q, Lu L, Tian J-H, Zhu Y-B, Qiao H and Sheng Z-H: Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons. Neuron. 68:73–86. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Castino R, Fiorentino I, Cagnin M, Giovia A and Isidoro C: Chelation of lysosomal iron protects dopaminergic SH-SY5Y neuroblastoma cells from hydrogen peroxide toxicity by precluding autophagy and Akt dephosphorylation. Toxicol Sci. 123:523–541. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Michiorri S, Gelmetti V, Giarda E, Lombardi F, Romano F, Marongiu R, Nerini-Molteni S, Sale P, Vago R, Arena G, et al: The Parkinson-associated protein PINK1 interacts with Beclin1 and promotes autophagy. Cell Death Differ. 17:962–974. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Youle RJ and Narendra DP: Mechanisms of mitophagy. Nat Rev Mol Cell Biol. 12:9–14. 2011. View Article : Google Scholar

42 

Nakahara K, Ueda M, Yamada K, Koide T, Yoshimochi G, Funayama M, Kim JH, Yamakawa S, Mori A, Misumi Y, et al: Juvenile-onset Parkinsonism with digenic parkin and PINK1 mutations treated with subthalamic nucleus stimulation at 45 years after disease onset. J Neurol Sci. 345:276–277. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Scarffe LA, Stevens DA, Dawson VL and Dawson TM: Parkin and PINK1: Much more than mitophagy. Trends Neurosci. 37:315–324. 2014. View Article : Google Scholar : PubMed/NCBI

44 

Exner N, Treske B, Paquet D, Holmström K, Schiesling C, Gispert S, Carballo-Carbajal I, Berg D, Hoepken HH, Gasser T, et al: Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. J Neurosci. 27:12413–12418. 2007. View Article : Google Scholar : PubMed/NCBI

45 

Dagda RK, Cherra SJ III, Kulich SM, Tandon A, Park D and Chu CT: Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission. J Biol Chem. 284:13843–13855. 2009. View Article : Google Scholar : PubMed/NCBI

46 

Twig G, Elorza A, Molina AJ, Mohamed H, Wikstrom JD, Walzer G, Stiles L, Haigh SE, Katz S, Las G, et al: Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J. 27:433–446. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Santin G, Piccolini VM, Barni S, Veneroni P, Giansanti V, Dal Bo V, Bernocchi G and Bottone MG: Mitochondrial fusion: A mechanism of cisplatin-induced resistance in neuroblastoma cells? Neurotoxicology. 34:51–60. 2013. View Article : Google Scholar

48 

Cipolat S, Martins de Brito O, Dal Zilio B and Scorrano L: OPA1 requires mitofusin 1 to promote mitochondrial fusion. Proc Natl Acad Sci USA. 101:15927–15932. 2004. View Article : Google Scholar : PubMed/NCBI

49 

Zorzano A, Liesa M, Sebastián D, Segalés J and Palacín M: Mitochondrial fusion proteins: Dual regulators of morphology and metabolism. Semin Cell Dev Biol. 21:566–574. 2010. View Article : Google Scholar : PubMed/NCBI

50 

Bertholet AM, Millet AME, Guillermin O, Daloyau M, Davezac N, Miquel M-C and Belenguer P: OPA1 loss of function affects in vitro neuronal maturation. Brain. 136:1518–1533. 2013. View Article : Google Scholar : PubMed/NCBI

51 

Duvezin-Caubet S, Jagasia R, Wagener J, Hofmann S, Trifunovic A, Hansson A, Chomyn A, Bauer MF, Attardi G, Larsson NG, et al: Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem. 281:37972–37979. 2006. View Article : Google Scholar : PubMed/NCBI

52 

Kopek BG, Shtengel G, Grimm JB, Clayton DA and Hess HF: Correlative photoactivated localization and scanning electron microscopy. PLoS One. 8:e772092013. View Article : Google Scholar : PubMed/NCBI

53 

Schaffer S, Gruber J, Ng LF, Fong S, Wong YT, Tang SY and Halliwell B: The effect of dichloroacetate on health- and lifespan in C. elegans. Biogerontology. 12:195–209. 2011. View Article : Google Scholar

54 

Gandhi S, Muqit MMK, Stanyer L, Healy DG, Abou-Sleiman PM, Hargreaves I, Heales S, Ganguly M, Parsons L, Lees AJ, et al: PINK1 protein in normal human brain and Parkinson’s disease. Brain. 129:1720–1731. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pajuelo-Reguera D, Alán L, Olejár T and Ježek P: Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells. Int J Oncol 46: 2409-2418, 2015.
APA
Pajuelo-Reguera, D., Alán, L., Olejár, T., & Ježek, P. (2015). Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells. International Journal of Oncology, 46, 2409-2418. https://doi.org/10.3892/ijo.2015.2953
MLA
Pajuelo-Reguera, D., Alán, L., Olejár, T., Ježek, P."Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells". International Journal of Oncology 46.6 (2015): 2409-2418.
Chicago
Pajuelo-Reguera, D., Alán, L., Olejár, T., Ježek, P."Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells". International Journal of Oncology 46, no. 6 (2015): 2409-2418. https://doi.org/10.3892/ijo.2015.2953
Copy and paste a formatted citation
x
Spandidos Publications style
Pajuelo-Reguera D, Alán L, Olejár T and Ježek P: Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells. Int J Oncol 46: 2409-2418, 2015.
APA
Pajuelo-Reguera, D., Alán, L., Olejár, T., & Ježek, P. (2015). Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells. International Journal of Oncology, 46, 2409-2418. https://doi.org/10.3892/ijo.2015.2953
MLA
Pajuelo-Reguera, D., Alán, L., Olejár, T., Ježek, P."Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells". International Journal of Oncology 46.6 (2015): 2409-2418.
Chicago
Pajuelo-Reguera, D., Alán, L., Olejár, T., Ježek, P."Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells". International Journal of Oncology 46, no. 6 (2015): 2409-2418. https://doi.org/10.3892/ijo.2015.2953
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